{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T11:31:36Z","timestamp":1792150296230,"version":"4.4.0"},"reference-count":34,"publisher":"American Society of Hematology","issue":"8","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2023,2,23]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Mutant calreticulin (CALR) proteins resulting from a\u00a0\u22121\/+2 frameshifting mutation of the CALR exon 9 carry a novel C-terminal amino acid sequence and drive the development of myeloproliferative neoplasms (MPNs). Mutant CALRs were shown to interact with and activate the thrombopoietin receptor (TpoR\/MPL) in the same cell. We report that mutant CALR proteins are secreted and can be found in patient plasma at levels up to 160 ng\/mL, with a mean of 25.64 ng\/mL. Plasma mutant CALR is found in complex with soluble transferrin receptor 1 (sTFR1) that acts as a carrier protein and increases mutant CALR half-life. Recombinant mutant CALR proteins bound and activated the TpoR in cell lines and primary megakaryocytic progenitors from patients with mutated CALR in which they drive thrombopoietin-independent colony formation. Importantly, the CALR-sTFR1 complex remains functional for TpoR activation. By bioluminescence resonance energy transfer assay, we show that mutant CALR proteins produced in 1 cell can specifically interact in trans with the TpoR on a target cell. In comparison with cells that only carry TpoR, cells that carry both TpoR and mutant CALR are hypersensitive to exogenous mutant CALR proteins and respond to levels of mutant CALR proteins similar to those in patient plasma. This is consistent with CALR-mutated cells that expose TpoR carrying immature N-linked sugars at the cell surface. Thus, secreted mutant CALR proteins will act more specifically on the MPN clone. In conclusion, a chaperone, CALR, can turn into a rogue cytokine through somatic mutation of its encoding gene.<\/jats:p>","DOI":"10.1182\/blood.2022016846","type":"journal-article","created":{"date-parts":[[2022,11,10]],"date-time":"2022-11-10T16:56:00Z","timestamp":1668099360000},"page":"917-929","update-policy":"https:\/\/doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":40,"title":["Secreted mutant calreticulins as rogue cytokines in\u00a0myeloproliferative neoplasms"],"prefix":"10.1182","volume":"141","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8623-3483","authenticated-orcid":false,"given":"Christian","family":"Pecquet","sequence":"first","affiliation":[{"name":"1Ludwig Cancer Research, Brussels, Belgium"},{"name":"2Universit\u00e9 Catholique de Louvain and de Duve Institute, SIGN Unit, Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7869-862X","authenticated-orcid":false,"given":"Nicolas","family":"Papadopoulos","sequence":"additional","affiliation":[{"name":"1Ludwig Cancer Research, Brussels, Belgium"},{"name":"2Universit\u00e9 Catholique de Louvain and de Duve Institute, SIGN Unit, Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0156-8936","authenticated-orcid":false,"given":"Thomas","family":"Balligand","sequence":"additional","affiliation":[{"name":"1Ludwig Cancer Research, Brussels, Belgium"},{"name":"2Universit\u00e9 Catholique de Louvain and de Duve Institute, SIGN Unit, Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5112-5394","authenticated-orcid":false,"given":"Ilyas","family":"Chachoua","sequence":"additional","affiliation":[{"name":"1Ludwig Cancer Research, Brussels, Belgium"},{"name":"2Universit\u00e9 Catholique de Louvain and de Duve Institute, SIGN Unit, Brussels, Belgium"},{"name":"3Department of Molecular Biology and Genetics, Bilkent University, Ankara, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amandine","family":"Tisserand","sequence":"additional","affiliation":[{"name":"4INSERM, Unit\u00e9 Mixte de Recherche (UMR) 1287, Gustave Roussy, Villejuif, France"},{"name":"5Universit\u00e9 Paris Cit\u00e9, UMR 1287, Gustave Roussy, Villejuif, France"},{"name":"6UMR 1287, Gustave Roussy, Villejuif, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ga\u00eblle","family":"Vertenoeil","sequence":"additional","affiliation":[{"name":"1Ludwig Cancer Research, Brussels, Belgium"},{"name":"2Universit\u00e9 Catholique de Louvain and de Duve Institute, SIGN Unit, Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Audrey","family":"N\u00e9d\u00e9lec","sequence":"additional","affiliation":[{"name":"1Ludwig Cancer Research, Brussels, Belgium"},{"name":"2Universit\u00e9 Catholique de Louvain and de Duve Institute, SIGN Unit, Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7648-8282","authenticated-orcid":false,"given":"Didier","family":"Vertommen","sequence":"additional","affiliation":[{"name":"2Universit\u00e9 Catholique de Louvain and de Duve Institute, SIGN Unit, Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7297-2920","authenticated-orcid":false,"given":"Anita","family":"Roy","sequence":"additional","affiliation":[{"name":"1Ludwig Cancer Research, Brussels, Belgium"},{"name":"2Universit\u00e9 Catholique de Louvain and de Duve Institute, SIGN Unit, Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4350-2302","authenticated-orcid":false,"given":"Caroline","family":"Marty","sequence":"additional","affiliation":[{"name":"4INSERM, Unit\u00e9 Mixte de Recherche (UMR) 1287, Gustave Roussy, Villejuif, France"},{"name":"6UMR 1287, Gustave Roussy, Villejuif, France"},{"name":"7Universit\u00e9 Paris-Saclay, UMR 1287, Gustave Roussy, Villejuif, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harini","family":"Nivarthi","sequence":"additional","affiliation":[{"name":"8CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Philippe","family":"Defour","sequence":"additional","affiliation":[{"name":"1Ludwig Cancer Research, Brussels, Belgium"},{"name":"2Universit\u00e9 Catholique de Louvain and de Duve Institute, SIGN Unit, Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mira","family":"El-Khoury","sequence":"additional","affiliation":[{"name":"4INSERM, Unit\u00e9 Mixte de Recherche (UMR) 1287, Gustave Roussy, Villejuif, France"},{"name":"6UMR 1287, Gustave Roussy, Villejuif, France"},{"name":"7Universit\u00e9 Paris-Saclay, UMR 1287, Gustave Roussy, Villejuif, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Hug","sequence":"additional","affiliation":[{"name":"9MyeloPro Diagnostics and Research GmbH, Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Majoros","sequence":"additional","affiliation":[{"name":"9MyeloPro Diagnostics and Research GmbH, Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erica","family":"Xu","sequence":"additional","affiliation":[{"name":"9MyeloPro Diagnostics and Research GmbH, Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oleh","family":"Zagrijtschuk","sequence":"additional","affiliation":[{"name":"10MyeloPro Diagnostics and Research GmbH, Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0251-3865","authenticated-orcid":false,"given":"Tudor E.","family":"Fertig","sequence":"additional","affiliation":[{"name":"10MyeloPro Diagnostics and Research GmbH, Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1475-6020","authenticated-orcid":false,"given":"Daciana S.","family":"Marta","sequence":"additional","affiliation":[{"name":"11Ultrastructural Pathology Lab and Bioimaging, Institute of Pathology Victor Babe\u0219, Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heinz","family":"Gisslinger","sequence":"additional","affiliation":[{"name":"12Division of Hematology and Blood Coagulation, Department of Internal Medicine I, Division of Hematology and Blood Coagulation, Medical University of Vienna, Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bettina","family":"Gisslinger","sequence":"additional","affiliation":[{"name":"12Division of Hematology and Blood Coagulation, Department of Internal Medicine I, Division of Hematology and Blood Coagulation, Medical University of Vienna, Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Schalling","sequence":"additional","affiliation":[{"name":"12Division of Hematology and Blood Coagulation, Department of Internal Medicine I, Division of Hematology and Blood Coagulation, Medical University of Vienna, Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5232-9543","authenticated-orcid":false,"given":"Ilaria","family":"Casetti","sequence":"additional","affiliation":[{"name":"13Division of Hematology, Fondazione IRCCS Policlinico San Matteo and University of Pavia, Pavia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7572-9504","authenticated-orcid":false,"given":"Elisa","family":"Rumi","sequence":"additional","affiliation":[{"name":"13Division of Hematology, Fondazione IRCCS Policlinico San Matteo and University of Pavia, Pavia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6575-8766","authenticated-orcid":false,"given":"Daniela","family":"Pietra","sequence":"additional","affiliation":[{"name":"13Division of Hematology, Fondazione IRCCS Policlinico San Matteo and University of Pavia, Pavia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiara","family":"Cavalloni","sequence":"additional","affiliation":[{"name":"13Division of Hematology, Fondazione IRCCS Policlinico San Matteo and University of Pavia, Pavia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luca","family":"Arcaini","sequence":"additional","affiliation":[{"name":"13Division of Hematology, Fondazione IRCCS Policlinico San Matteo and University of Pavia, Pavia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6984-8817","authenticated-orcid":false,"given":"Mario","family":"Cazzola","sequence":"additional","affiliation":[{"name":"13Division of Hematology, Fondazione IRCCS Policlinico San Matteo and University of Pavia, Pavia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1880-9126","authenticated-orcid":false,"given":"Norio","family":"Komatsu","sequence":"additional","affiliation":[{"name":"14Department of Hematology, Graduate School of Medicine, Juntendo University, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshihiko","family":"Kihara","sequence":"additional","affiliation":[{"name":"14Department of Hematology, Graduate School of Medicine, Juntendo University, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshitaka","family":"Sunami","sequence":"additional","affiliation":[{"name":"14Department of Hematology, Graduate School of Medicine, Juntendo University, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoko","family":"Edahiro","sequence":"additional","affiliation":[{"name":"14Department of Hematology, Graduate School of Medicine, Juntendo University, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3502-5000","authenticated-orcid":false,"given":"Marito","family":"Araki","sequence":"additional","affiliation":[{"name":"15Department of Transfusion Medicine and Stem Cell Regulation, Graduate School of Medicine, Juntendo University, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3322-0080","authenticated-orcid":false,"given":"Roman","family":"Lesyk","sequence":"additional","affiliation":[{"name":"16Department of Biotechnology and Cell Biology, Medical College, University of Information Technology and Management in Rzeszow, Rzeszow, Poland"},{"name":"17Department of Pharmaceutical, Organic and Bioorganic Chemistry, Danylo Halytsky Lviv National Medical University, Lviv, Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7369-9700","authenticated-orcid":false,"given":"Veronika","family":"Buxhofer-Ausch","sequence":"additional","affiliation":[{"name":"18Department of Internal Medicine I with Hematology, Ordensklinikum Linz Elisabethinen, Stem Cell Transplantation Hemostaseology and Medical Oncology, Linz, Austria"},{"name":"19Medical Faculty, Johannes Kepler University Linz, Linz, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8182-990X","authenticated-orcid":false,"given":"Sonja","family":"Heibl","sequence":"additional","affiliation":[{"name":"20Department of Internal Medicine IV, Klinikum Wels-Grieskirchen, Wels, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florence","family":"Pasquier","sequence":"additional","affiliation":[{"name":"4INSERM, Unit\u00e9 Mixte de Recherche (UMR) 1287, Gustave Roussy, Villejuif, France"},{"name":"5Universit\u00e9 Paris Cit\u00e9, UMR 1287, Gustave Roussy, Villejuif, France"},{"name":"6UMR 1287, Gustave Roussy, Villejuif, France"},{"name":"21Department of Hematology, Gustave Roussy, Villejuif, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Violaine","family":"Havelange","sequence":"additional","affiliation":[{"name":"2Universit\u00e9 Catholique de Louvain and de Duve Institute, SIGN Unit, Brussels, Belgium"},{"name":"22Department of Hematology, Cliniques universitaires Saint-Luc, Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5915-6910","authenticated-orcid":false,"given":"Isabelle","family":"Plo","sequence":"additional","affiliation":[{"name":"4INSERM, Unit\u00e9 Mixte de Recherche (UMR) 1287, Gustave Roussy, Villejuif, France"},{"name":"6UMR 1287, Gustave Roussy, Villejuif, France"},{"name":"7Universit\u00e9 Paris-Saclay, UMR 1287, Gustave Roussy, Villejuif, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4705-202X","authenticated-orcid":false,"given":"William","family":"Vainchenker","sequence":"additional","affiliation":[{"name":"4INSERM, Unit\u00e9 Mixte de Recherche (UMR) 1287, Gustave Roussy, Villejuif, France"},{"name":"6UMR 1287, Gustave Roussy, Villejuif, France"},{"name":"7Universit\u00e9 Paris-Saclay, UMR 1287, Gustave Roussy, Villejuif, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6997-8539","authenticated-orcid":false,"given":"Robert","family":"Kralovics","sequence":"additional","affiliation":[{"name":"23Department of Laboratory Medicine, Medical University of Vienna, Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8599-2699","authenticated-orcid":false,"given":"Stefan N.","family":"Constantinescu","sequence":"additional","affiliation":[{"name":"1Ludwig Cancer Research, Brussels, Belgium"},{"name":"2Universit\u00e9 Catholique de Louvain and de Duve Institute, SIGN Unit, Brussels, Belgium"},{"name":"24Walloon Excellence in Life Sciences and Biotechnology, Brussels, Belgium"},{"name":"25Ludwig Institute for Cancer Research, Nuffield Department of Medicine, Oxford University, Oxford, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"234","reference":[{"issue":"25","key":"2023092917595846900_bib1","doi-asserted-by":"crossref","first-page":"2391","DOI":"10.1056\/NEJMoa1312542","article-title":"Somatic CALR mutations in myeloproliferative neoplasms with nonmutated JAK2","volume":"369","author":"Nangalia","year":"2013","journal-title":"N\u00a0Engl J Med"},{"issue":"25","key":"2023092917595846900_bib2","doi-asserted-by":"crossref","first-page":"2379","DOI":"10.1056\/NEJMoa1311347","article-title":"Somatic mutations of calreticulin in myeloproliferative neoplasms","volume":"369","author":"Klampfl","year":"2013","journal-title":"N\u00a0Engl J Med"},{"issue":"3","key":"2023092917595846900_bib3","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1042\/BJ20081847","article-title":"Calreticulin, a multi-process calcium-buffering chaperone of the endoplasmic reticulum","volume":"417","author":"Michalak","year":"2009","journal-title":"Biochem J"},{"issue":"10","key":"2023092917595846900_bib4","doi-asserted-by":"crossref","first-page":"1325","DOI":"10.1182\/blood-2015-11-681932","article-title":"Thrombopoietin receptor activation by myeloproliferative neoplasm associated calreticulin mutants","volume":"127","author":"Chachoua","year":"2016","journal-title":"Blood"},{"issue":"8","key":"2023092917595846900_bib5","doi-asserted-by":"crossref","first-page":"1759","DOI":"10.1038\/leu.2016.32","article-title":"Thrombopoietin receptor is required for the oncogenic function of CALR mutants","volume":"30","author":"Nivarthi","year":"2016","journal-title":"Leukemia"},{"issue":"7","key":"2023092917595846900_bib6","doi-asserted-by":"crossref","first-page":"782","DOI":"10.1182\/blood-2017-08-800896","article-title":"Defining the requirements for the pathogenic interaction between mutant calreticulin and\u00a0MPL in MPN","volume":"131","author":"Elf","year":"2018","journal-title":"Blood"},{"issue":"4","key":"2023092917595846900_bib7","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1158\/2159-8290.CD-15-1434","article-title":"Mutant Calreticulin Requires Both Its Mutant C-terminus and the Thrombopoietin Receptor for Oncogenic Transformation","volume":"6","author":"Elf","year":"2016","journal-title":"Cancer Discov"},{"issue":"10","key":"2023092917595846900_bib8","doi-asserted-by":"crossref","first-page":"1317","DOI":"10.1182\/blood-2015-11-679571","article-title":"Calreticulin mutants in mice induce an MPL-dependent thrombocytosis with frequent progression to myelofibrosis","volume":"127","author":"Marty","year":"2016","journal-title":"Blood"},{"issue":"10","key":"2023092917595846900_bib9","doi-asserted-by":"crossref","first-page":"1307","DOI":"10.1182\/blood-2015-09-671172","article-title":"Activation of the thrombopoietin receptor by mutant calreticulin in CALR-mutant myeloproliferative neoplasms","volume":"127","author":"Araki","year":"2016","journal-title":"Blood"},{"issue":"8","key":"2023092917595846900_bib10","doi-asserted-by":"crossref","first-page":"1775","DOI":"10.1038\/leu.2016.47","article-title":"Pathologic activation of thrombopoietin receptor and JAK2-STAT5 pathway by frameshift mutants of mouse calreticulin","volume":"30","author":"Balligand","year":"2016","journal-title":"Leukemia"},{"issue":"1","key":"2023092917595846900_bib11","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1186\/s13045-016-0275-0","article-title":"Calreticulin-mutant proteins induce megakaryocytic signaling to transform hematopoietic cells and undergo accelerated degradation and Golgi-mediated secretion","volume":"9","author":"Han","year":"2016","journal-title":"J\u00a0Hematol Oncol"},{"issue":"25","key":"2023092917595846900_bib12","doi-asserted-by":"crossref","first-page":"2669","DOI":"10.1182\/blood-2018-09-874578","article-title":"Calreticulin mutants as oncogenic rogue chaperones for TpoR and traffic-defective pathogenic TpoR mutants","volume":"133","author":"Pecquet","year":"2019","journal-title":"Blood"},{"issue":"1","key":"2023092917595846900_bib13","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1038\/s41375-018-0181-2","article-title":"Homomultimerization of mutant calreticulin is a prerequisite for MPL binding and activation","volume":"33","author":"Araki","year":"2018","journal-title":"Leukemia"},{"issue":"2","key":"2023092917595846900_bib14","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1038\/s41375-019-0538-1","article-title":"Knock-in of murine Calr del52 induces essential thrombocythemia with slow-rising dominance in mice and reveals key role of Calr exon 9 in cardiac development","volume":"34","author":"Balligand","year":"2020","journal-title":"Leukemia"},{"issue":"suppl 1","key":"2023092917595846900_bib15","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1182\/blood-2018-99-118348","article-title":"Secreted mutant calreticulins as rogue cytokines trigger thrombopoietin receptor activation specifically in CALR mutated cells: perspectives for MPN therapy","volume":"132","author":"Pecquet","year":"2018","journal-title":"Blood"},{"issue":"4","key":"2023092917595846900_bib16","doi-asserted-by":"crossref","first-page":"748","DOI":"10.1016\/j.molcel.2019.11.004","article-title":"Immunosuppression by mutated calreticulin released from malignant cells","volume":"77","author":"Liu","year":"2019","journal-title":"Mol Cell"},{"issue":"1","key":"2023092917595846900_bib17","doi-asserted-by":"crossref","first-page":"4886","DOI":"10.1038\/s41467-020-18691-3","article-title":"Calreticulin del52 and ins5 knock-in mice recapitulate different myeloproliferative phenotypes observed in patients with MPN","volume":"11","author":"Benlabiod","year":"2020","journal-title":"Nat Commun"},{"issue":"1","key":"2023092917595846900_bib18","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1084\/jem.177.1.1","article-title":"The calcium-binding protein calreticulin is a major constituent of lytic granules in cytolytic T lymphocytes","volume":"177","author":"Dupuis","year":"1993","journal-title":"J\u00a0Exp Med"},{"issue":"3","key":"2023092917595846900_bib19","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1006\/clin.1994.1160","article-title":"Calreticulin is released from activated neutrophils and binds to C1q and mannan-binding protein","volume":"72","author":"Eggleton","year":"1994","journal-title":"Clin Immunol Immunopathol"},{"issue":"6","key":"2023092917595846900_bib20","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1084\/jem.194.6.781","article-title":"C1q and mannose binding lectin engagement of cell surface calreticulin and Cd91 initiates macropinocytosis and uptake of apoptotic cells","volume":"194","author":"Ogden","year":"2001","journal-title":"J\u00a0Exp Med"},{"issue":"2","key":"2023092917595846900_bib21","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/j.cell.2005.08.032","article-title":"Cell-surface calreticulin initiates clearance of viable or apoptotic cells through trans-activation of LRP on the phagocyte","volume":"123","author":"Gardai","year":"2005","journal-title":"Cell"},{"issue":"7","key":"2023092917595846900_bib22","doi-asserted-by":"crossref","first-page":"2145","DOI":"10.1073\/pnas.1424907112","article-title":"Macrophages eat cancer cells using their own calreticulin as a guide: roles of TLR and Btk","volume":"112","author":"Feng","year":"2015","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"1","key":"2023092917595846900_bib23","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/S0009-8981(03)00005-6","article-title":"Soluble transferrin receptor for the evaluation of erythropoiesis and iron status","volume":"329","author":"Beguin","year":"2003","journal-title":"Clin Chim Acta"},{"issue":"2","key":"2023092917595846900_bib24","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1093\/glycob\/5.2.227","article-title":"The critical glycosylation site of human transferrin receptor contains a high-mannose oligosaccharide","volume":"5","author":"Hayes","year":"1995","journal-title":"Glycobiology"},{"key":"2023092917595846900_bib25","volume-title":"Clinical Pharmacokinetics\/Pharmacodynamics","author":"Rowland","year":"2005"},{"issue":"suppl 1","key":"2023092917595846900_bib26","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1002\/stem.5530140732","article-title":"In\u00a0vivo biological effects of various forms of thrombopoietin in a murine model of transient pancytopenia","volume":"14","author":"Thomas","year":"1996","journal-title":"Stem Cells"},{"key":"2023092917595846900_bib27","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.exphem.2017.09.006","article-title":"Persistent elevation of plasma thrombopoietin levels after treatment in severe aplastic anemia","volume":"58","author":"Zhao","year":"2018","journal-title":"Exp Hematol"},{"issue":"1-2","key":"2023092917595846900_bib28","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/S0303-7207(97)00075-0","article-title":"Specificity of transcription enhancement via the STAT responsive element in the serine protease inhibitor 2.1 promoter","volume":"130","author":"Wood","year":"1997","journal-title":"Mol Cell Endocrinol"},{"issue":"8","key":"2023092917595846900_bib29","doi-asserted-by":"crossref","first-page":"2071","DOI":"10.3324\/haematol.2019.224899","article-title":"Transferrin receptor 1-mediated iron uptake plays\u00a0an\u00a0essential role in hematopoiesis","volume":"105","author":"Wang","year":"2020","journal-title":"Haematologica"},{"issue":"19","key":"2023092917595846900_bib30","doi-asserted-by":"crossref","first-page":"13480","DOI":"10.1074\/jbc.274.19.13480","article-title":"Thrombopoietin signal transduction requires functional JAK2, not TYK2","volume":"274","author":"Drachman","year":"1999","journal-title":"J\u00a0Biol Chem"},{"issue":"2","key":"2023092917595846900_bib31","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1042\/bj3440281","article-title":"Calreticulin: one protein, one gene, many functions","volume":"344","author":"Michalak","year":"1999","journal-title":"Biochem J"},{"issue":"1","key":"2023092917595846900_bib32","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1021\/bi0355286","article-title":"Mutational analysis provides molecular insight into the carbohydrate-binding region of calreticulin: pivotal roles of tyrosine-109 and aspartate-135 in carbohydrate recognition","volume":"43","author":"Kapoor","year":"2004","journal-title":"Biochemistry"},{"issue":"suppl 1","key":"2023092917595846900_bib33","doi-asserted-by":"crossref","first-page":"3593","DOI":"10.1182\/blood-2021-153480","article-title":"Type I calreticulin mutations result in hyperactivation of its acetyltransferase function and iron metabolism, inducing a susceptibility to ferroptosis","volume":"138","author":"Greenbaum","year":"2021","journal-title":"Blood"},{"issue":"6","key":"2023092917595846900_bib34","doi-asserted-by":"crossref","first-page":"698","DOI":"10.1002\/ajh.26171","article-title":"Hematopoietic expression of a chimeric murine-human CALR oncoprotein allows the assessment of anti-CALR antibody immunotherapies in\u00a0vivo","volume":"96","author":"Achyutuni","year":"2021","journal-title":"Am J Hematol"}],"container-title":["Blood"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/ashpublications.org\/blood\/article-pdf\/141\/8\/917\/2082545\/blood_bld-2022-016846-main.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/ashpublications.org\/blood\/article-pdf\/141\/8\/917\/2082545\/blood_bld-2022-016846-main.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T14:00:15Z","timestamp":1695996015000},"score":1,"resource":{"primary":{"URL":"https:\/\/ashpublications.org\/blood\/article\/141\/8\/917\/487064\/Secreted-mutant-calreticulins-as-rogue-cytokines"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,2,23]]},"references-count":34,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2023,2,23]]}},"URL":"https:\/\/doi.org\/10.1182\/blood.2022016846","relation":{},"ISSN":["0006-4971","1528-0020"],"issn-type":[{"value":"0006-4971","type":"print"},{"value":"1528-0020","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,2,23]]}}}