{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,6]],"date-time":"2026-11-06T02:12:57Z","timestamp":1793931177367,"version":"4.8.0"},"reference-count":80,"publisher":"American Society of Hematology","issue":"13","license":[{"start":{"date-parts":[[2023,6,27]],"date-time":"2023-06-27T00:00:00Z","timestamp":1687824000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2023,9,28]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Although absence of interleukin-7 (IL-7) signaling completely abrogates T and B lymphopoiesis in mice, patients with severe combined immunodeficiency caused by mutations in the IL-7 receptor \u03b1 chain (IL-7R\u03b1) still generate peripheral blood B cells. Consequently, human B lymphopoiesis has been thought to be independent of IL-7 signaling. Using flow cytometric analysis and single-cell RNA sequencing of bone marrow samples from healthy controls and patients who are IL-7R\u03b1 deficient, in combination with in\u00a0vitro modeling of human B-cell differentiation, we demonstrate that IL-7R signaling plays a crucial role in human B lymphopoiesis. IL-7 drives proliferation and expansion of early B-cell progenitors but not of pre-BII large cells and has a limited role in the prevention of cell death. Furthermore, IL-7 guides cell fate decisions by enhancing the expression of BACH2, EBF1, and PAX5, which jointly orchestrate the specification and commitment of early B-cell progenitors. In line with this observation, early B-cell progenitors of patients with IL-7R\u03b1 deficiency still expressed myeloid-specific genes. Collectively, our results unveil a previously unknown role for IL-7 signaling in promoting the B-lymphoid fate and expanding early human B-cell progenitors while defining important differences between mice and humans. Our results have implications for hematopoietic stem cell transplantation strategies in patients with T\u2212 B+ severe combined immunodeficiency and provide insights into the role of IL-7R signaling in leukemogenesis.<\/jats:p>","DOI":"10.1182\/blood.2023019721","type":"journal-article","created":{"date-parts":[[2023,6,27]],"date-time":"2023-06-27T19:53:50Z","timestamp":1687895630000},"page":"1113-1130","update-policy":"https:\/\/doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":67,"title":["IL-7 receptor signaling drives human B-cell progenitor differentiation and expansion"],"prefix":"10.1182","volume":"142","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0335-2859","authenticated-orcid":false,"given":"Fabian M. P.","family":"Kaiser","sequence":"first","affiliation":[{"name":"1Department of Immunology, Erasmus University Medical Center, Rotterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iga","family":"Janowska","sequence":"additional","affiliation":[{"name":"2Department of Rheumatology and Clinical Immunology, Freiburg University Medical Center, University of Freiburg, Freiburg, Germany"},{"name":"3Center for Chronic Immunodeficiency, University Medical Center Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5266-0639","authenticated-orcid":false,"given":"Roberta","family":"Menafra","sequence":"additional","affiliation":[{"name":"4Leiden Genome Technology Center, Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2868-0127","authenticated-orcid":false,"given":"Melanie","family":"de Gier","sequence":"additional","affiliation":[{"name":"5Department of Pediatrics, Laboratory for Pediatric Immunology, Willem-Alexander Children\u2019s Hospital, Leiden University Medical Center, Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4055-525X","authenticated-orcid":false,"given":"Jakov","family":"Korzhenevich","sequence":"additional","affiliation":[{"name":"6Division of Clinical and Experimental Immunology, Institute of Immunology, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingrid","family":"Pico-Knijnenburg","sequence":"additional","affiliation":[{"name":"5Department of Pediatrics, Laboratory for Pediatric Immunology, Willem-Alexander Children\u2019s Hospital, Leiden University Medical Center, Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Indu","family":"Khatri","sequence":"additional","affiliation":[{"name":"7Department of Immunology, Leiden University Medical Center, Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ansgar","family":"Schulz","sequence":"additional","affiliation":[{"name":"8Department of Pediatrics and Adolescent Medicine, University Medical Center, University Ulm, Ulm, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7421-3370","authenticated-orcid":false,"given":"Taco W.","family":"Kuijpers","sequence":"additional","affiliation":[{"name":"9Department of Pediatrics, Amsterdam University Medical Center, Amsterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arjan C.","family":"Lankester","sequence":"additional","affiliation":[{"name":"10Department of Pediatrics, Hematology and Stem Cell Transplantation, Willem-Alexander Children\u2019s Hospital, Leiden University Medical Center, Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lukas","family":"Konstantinidis","sequence":"additional","affiliation":[{"name":"11Department of Orthopedics and Trauma Surgery, Freiburg University Medical Center, University of Freiburg, Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7261-1447","authenticated-orcid":false,"given":"Miriam","family":"Erlacher","sequence":"additional","affiliation":[{"name":"12Division of Pediatric Hematology and Oncology, Department of Pediatrics and Adolescent Medicine, Freiburg University Medical Center, University of Freiburg, Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9189-0640","authenticated-orcid":false,"given":"Susan","family":"Kloet","sequence":"additional","affiliation":[{"name":"4Leiden Genome Technology Center, Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pauline A.","family":"van Schouwenburg","sequence":"additional","affiliation":[{"name":"5Department of Pediatrics, Laboratory for Pediatric Immunology, Willem-Alexander Children\u2019s Hospital, Leiden University Medical Center, Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5153-6089","authenticated-orcid":false,"given":"Marta","family":"Rizzi","sequence":"additional","affiliation":[{"name":"2Department of Rheumatology and Clinical Immunology, Freiburg University Medical Center, University of Freiburg, Freiburg, Germany"},{"name":"3Center for Chronic Immunodeficiency, University Medical Center Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany"},{"name":"6Division of Clinical and Experimental Immunology, Institute of Immunology, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria"},{"name":"13Centre for Integrative Biological Signalling Studies, University of Freiburg, Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1510-3104","authenticated-orcid":false,"given":"Mirjam","family":"van der Burg","sequence":"additional","affiliation":[{"name":"5Department of Pediatrics, Laboratory for Pediatric Immunology, Willem-Alexander Children\u2019s Hospital, Leiden University Medical Center, Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"234","reference":[{"issue":"6","key":"2023092815303308500_bib1","doi-asserted-by":"crossref","first-page":"1219","DOI":"10.1016\/j.immuni.2016.11.004","article-title":"Hematopoietic stem cell niches produce lineage-instructive signals to control multipotent progenitor differentiation","volume":"45","author":"Cordeiro Gomes","year":"2016","journal-title":"Immunity"},{"issue":"1","key":"2023092815303308500_bib2","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1084\/jem.178.1.257","article-title":"Inhibition of murine B and T lymphopoiesis in\u00a0vivo by an anti-interleukin 7 monoclonal antibody","volume":"178","author":"Grabstein","year":"1993","journal-title":"J\u00a0Exp Med"},{"issue":"5","key":"2023092815303308500_bib3","doi-asserted-by":"crossref","first-page":"1955","DOI":"10.1084\/jem.180.5.1955","article-title":"Early lymphocyte expansion is severely impaired in interleukin 7 receptor-deficient mice","volume":"180","author":"Peschon","year":"1994","journal-title":"J\u00a0Exp Med"},{"issue":"4","key":"2023092815303308500_bib4","doi-asserted-by":"crossref","first-page":"1519","DOI":"10.1084\/jem.181.4.1519","article-title":"Lymphopenia in interleukin (IL)-7 gene-deleted mice identifies IL-7 as a nonredundant cytokine","volume":"181","author":"von Freeden-Jeffry","year":"1995","journal-title":"J\u00a0Exp Med"},{"issue":"5","key":"2023092815303308500_bib5","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1084\/jem.20020784","article-title":"The earliest step in B lineage differentiation from common lymphoid progenitors is critically dependent upon interleukin 7","volume":"196","author":"Miller","year":"2002","journal-title":"J\u00a0Exp Med"},{"issue":"8","key":"2023092815303308500_bib6","doi-asserted-by":"crossref","first-page":"1197","DOI":"10.1084\/jem.20050158","article-title":"IL-7 receptor signaling is necessary for stage transition in adult B cell development through up-regulation of EBF","volume":"201","author":"Kikuchi","year":"2005","journal-title":"J\u00a0Exp Med"},{"issue":"2","key":"2023092815303308500_bib7","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1038\/nri3570","article-title":"Orchestrating B cell lymphopoiesis through interplay of IL-7 receptor and pre-B cell receptor signalling","volume":"14","author":"Clark","year":"2014","journal-title":"Nat Rev Immunol"},{"issue":"5","key":"2023092815303308500_bib8","doi-asserted-by":"crossref","first-page":"1283","DOI":"10.1182\/blood-2011-01-332189","article-title":"IL-7 mediates Ebf-1-dependent lineage restriction in early lymphoid progenitors","volume":"118","author":"Tsapogas","year":"2011","journal-title":"Blood"},{"issue":"5","key":"2023092815303308500_bib9","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1038\/ni1324","article-title":"A\u00a0unique function for cyclin D3 in early B cell development","volume":"7","author":"Cooper","year":"2006","journal-title":"Nat Immunol"},{"issue":"10","key":"2023092815303308500_bib10","doi-asserted-by":"crossref","first-page":"1110","DOI":"10.1038\/ni.1785","article-title":"Ras orchestrates exit from the cell cycle and light-chain recombination during early B cell development","volume":"10","author":"Mandal","year":"2009","journal-title":"Nat Immunol"},{"issue":"1","key":"2023092815303308500_bib11","doi-asserted-by":"crossref","first-page":"eaar5701","DOI":"10.1126\/sciadv.aar5701","article-title":"Discrete roles and bifurcation of PTEN signaling and mTORC1-mediated anabolic metabolism underlie IL-7-driven B lymphopoiesis","volume":"4","author":"Zeng","year":"2018","journal-title":"Sci Adv"},{"issue":"4","key":"2023092815303308500_bib12","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1016\/S1074-7613(01)00216-3","article-title":"Pre-B cell receptor signaling mediates selective response to IL-7 at the pro-B to pre-B cell transition via an ERK\/MAP kinase-dependent pathway","volume":"15","author":"Fleming","year":"2001","journal-title":"Immunity"},{"issue":"8","key":"2023092815303308500_bib13","doi-asserted-by":"crossref","first-page":"4770","DOI":"10.4049\/jimmunol.172.8.4770","article-title":"STAT5 activation underlies il7 receptor-dependent B cell development","volume":"172","author":"Goetz","year":"2004","journal-title":"J\u00a0Immunol"},{"issue":"14","key":"2023092815303308500_bib14","doi-asserted-by":"crossref","first-page":"6501","DOI":"10.1128\/MCB.24.14.6501-6513.2004","article-title":"Distinct regions of the interleukin-7 receptor regulate different Bcl2 family members","volume":"24","author":"Jiang","year":"2004","journal-title":"Mol Cell Biol"},{"issue":"6","key":"2023092815303308500_bib15","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1093\/intimm\/dxp043","article-title":"Loss of the pro-apoptotic BH3-only Bcl-2 family member Bim sustains B lymphopoiesis in the absence of IL-7","volume":"21","author":"Huntington","year":"2009","journal-title":"Int Immunol"},{"issue":"2","key":"2023092815303308500_bib16","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1038\/ni.1827","article-title":"Role of STAT5 in controlling cell survival and immunoglobulin gene recombination during pro-B cell development","volume":"11","author":"Malin","year":"2010","journal-title":"Nat Immunol"},{"issue":"2","key":"2023092815303308500_bib17","doi-asserted-by":"crossref","first-page":"109349","DOI":"10.1016\/j.celrep.2021.109349","article-title":"IL-7R signaling activates widespread VH and DH gene usage to drive antibody diversity in bone marrow B cells","volume":"36","author":"Baizan-Edge","year":"2021","journal-title":"Cell Rep"},{"issue":"12","key":"2023092815303308500_bib18","doi-asserted-by":"crossref","first-page":"1212","DOI":"10.1038\/ni.2136","article-title":"Epigenetic repression of the Igk locus by STAT5-mediated recruitment of the histone methyltransferase Ezh2","volume":"12","author":"Mandal","year":"2011","journal-title":"Nat Immunol"},{"issue":"4","key":"2023092815303308500_bib19","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1038\/3877","article-title":"Defective IL7R expression in T-B+ NK+ severe combined immunodeficiency","volume":"20","author":"Puel","year":"1998","journal-title":"Nat Genet"},{"issue":"1","key":"2023092815303308500_bib20","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1111\/j.0105-2896.2005.00234.x","article-title":"Interleukin-7 receptor alpha (IL-7Ralpha) deficiency: cellular and molecular bases. Analysis of clinical, immunological, and molecular features in 16 novel patients","volume":"203","author":"Giliani","year":"2005","journal-title":"Immunol Rev"},{"issue":"19","key":"2023092815303308500_bib21","doi-asserted-by":"crossref","first-page":"10348","DOI":"10.1073\/pnas.93.19.10348","article-title":"Interleukin 7 independent development of human B cells","volume":"93","author":"Prieyl","year":"1996","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"11","key":"2023092815303308500_bib22","doi-asserted-by":"crossref","first-page":"7325","DOI":"10.4049\/jimmunol.175.11.7325","article-title":"Murine and human IL-7 activate STAT5 and induce proliferation of normal human pro-B cells","volume":"175","author":"Johnson","year":"2005","journal-title":"J\u00a0Immunol"},{"issue":"7","key":"2023092815303308500_bib23","doi-asserted-by":"crossref","first-page":"2262","DOI":"10.1073\/pnas.0511094103","article-title":"Appearance of immature\/transitional B cells in HIV-infected individuals with advanced disease: correlation with increased IL-7","volume":"103","author":"Malaspina","year":"2006","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"12","key":"2023092815303308500_bib24","doi-asserted-by":"crossref","first-page":"8109","DOI":"10.4049\/jimmunol.180.12.8109","article-title":"IL-7 activates the phosphatidylinositol 3-kinase\/AKT pathway in normal human thymocytes but not normal human B cell precursors","volume":"180","author":"Johnson","year":"2008","journal-title":"J\u00a0Immunol"},{"issue":"7","key":"2023092815303308500_bib25","doi-asserted-by":"crossref","first-page":"4255","DOI":"10.4049\/jimmunol.0800489","article-title":"IL-7 Dependence in human B lymphopoiesis increases during progression of ontogeny from cord blood to bone marrow","volume":"182","author":"Parrish","year":"2009","journal-title":"J\u00a0Immunol"},{"issue":"9","key":"2023092815303308500_bib26","doi-asserted-by":"crossref","first-page":"2155","DOI":"10.1002\/eji.201646307","article-title":"TSLP or IL-7 provide an IL-7R\u03b1 signal that is critical for human B lymphopoiesis","volume":"46","author":"Milford","year":"2016","journal-title":"Eur J Immunol"},{"key":"2023092815303308500_bib27","doi-asserted-by":"crossref","first-page":"33924","DOI":"10.1038\/srep33924","article-title":"Decreased IL7R\u03b1 and TdT expression underlie the skewed immunoglobulin repertoire of human B-cell precursors from fetal origin","volume":"6","author":"Rother","year":"2016","journal-title":"Sci Rep"},{"issue":"5","key":"2023092815303308500_bib28","doi-asserted-by":"crossref","first-page":"901","DOI":"10.1084\/jem.20110580","article-title":"Gain-of-function mutations in interleukin-7 receptor-\u03b1 (IL7R) in childhood acute lymphoblastic leukemias","volume":"208","author":"Shochat","year":"2011","journal-title":"J\u00a0Exp Med"},{"issue":"26","key":"2023092815303308500_bib29","doi-asserted-by":"crossref","first-page":"4259","DOI":"10.1182\/blood-2012-08-451278","article-title":"In\u00a0vivo leukemogenic potential of an interleukin 7 receptor \u03b1 chain mutant in hematopoietic stem and progenitor cells","volume":"122","author":"Yokoyama","year":"2013","journal-title":"Blood"},{"issue":"1","key":"2023092815303308500_bib30","doi-asserted-by":"crossref","first-page":"7268","DOI":"10.1038\/s41467-021-27197-5","article-title":"Interleukin-7 receptor \u03b1 mutational activation can initiate precursor B-cell acute lymphoblastic leukemia","volume":"12","author":"Almeida","year":"2021","journal-title":"Nat Commun"},{"issue":"1","key":"2023092815303308500_bib31","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1038\/s41375-021-01326-x","article-title":"Activated interleukin-7 receptor signaling drives B-cell acute lymphoblastic leukemia in mice","volume":"36","author":"Thomas","year":"2022","journal-title":"Leukemia"},{"issue":"1","key":"2023092815303308500_bib32","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1038\/s41467-022-28218-7","article-title":"An instructive role for interleukin-7 receptor \u03b1 in the development of human B-cell precursor leukemia","volume":"13","author":"Geron","year":"2022","journal-title":"Nat Commun"},{"issue":"4","key":"2023092815303308500_bib33","doi-asserted-by":"crossref","first-page":"994","DOI":"10.1016\/j.jaci.2013.01.047","article-title":"B-cell reconstitution for SCID: should a conditioning regimen be used in SCID treatment?","volume":"131","author":"Haddad","year":"2013","journal-title":"J\u00a0Allergy Clin Immunol"},{"issue":"23","key":"2023092815303308500_bib34","doi-asserted-by":"crossref","first-page":"3749","DOI":"10.1182\/blood-2013-02-380105","article-title":"How I treat severe combined immunodeficiency","volume":"122","author":"Gaspar","year":"2013","journal-title":"Blood"},{"key":"2023092815303308500_bib35","doi-asserted-by":"crossref","first-page":"2680","DOI":"10.3389\/fimmu.2019.02680","article-title":"Delineating human B cell precursor development with genetically identified PID cases as a model","volume":"10","author":"Wentink","year":"2019","journal-title":"Front Immunol"},{"issue":"3","key":"2023092815303308500_bib36","doi-asserted-by":"crossref","first-page":"1044","DOI":"10.4049\/jimmunol.1301815","article-title":"A\u00a0feeder-free differentiation system identifies autonomously proliferating B cell precursors in human bone marrow","volume":"192","author":"Kraus","year":"2014","journal-title":"J\u00a0Immunol"},{"issue":"7","key":"2023092815303308500_bib37","doi-asserted-by":"crossref","first-page":"1888","DOI":"10.1016\/j.cell.2019.05.031","article-title":"Comprehensive integration of single-cell data","volume":"177","author":"Stuart","year":"2019","journal-title":"Cell"},{"issue":"1","key":"2023092815303308500_bib38","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1186\/s12864-018-4772-0","article-title":"Slingshot: cell lineage and pseudotime inference for single-cell transcriptomics","volume":"19","author":"Street","year":"2018","journal-title":"BMC Genomics"},{"issue":"1","key":"2023092815303308500_bib39","doi-asserted-by":"crossref","first-page":"1201","DOI":"10.1038\/s41467-020-14766-3","article-title":"Trajectory-based differential expression analysis for single-cell sequencing data","volume":"11","author":"Van den Berge","year":"2020","journal-title":"Nat Commun"},{"key":"2023092815303308500_bib40","doi-asserted-by":"publisher","DOI":"10.1101\/2021.03.09.433671","article-title":"Trajectory inference across multiple conditions with condiments: differential topology, progression, differentiation, and expression","author":"de B\u00e9zieux","year":"10  2021","journal-title":"bioRxiv"},{"issue":"14","key":"2023092815303308500_bib41","doi-asserted-by":"crossref","first-page":"3791","DOI":"10.1158\/0008-5472.CAN-20-3562","article-title":"LSD1 and aberrant dna methylation mediate persistence of enteroendocrine progenitors that support BRAF-mutant colorectal cancer","volume":"81","author":"Miller","year":"2021","journal-title":"Cancer Res"},{"issue":"9","key":"2023092815303308500_bib42","doi-asserted-by":"crossref","first-page":"1467","DOI":"10.1038\/leu.2011.118","article-title":"Standardization of DNA isolation from low cell numbers for chimerism analysis by PCR of short tandem repeats","volume":"25","author":"van der Burg","year":"2011","journal-title":"Leukemia"},{"key":"2023092815303308500_bib43","doi-asserted-by":"crossref","first-page":"157","DOI":"10.3389\/fimmu.2015.00157","article-title":"Strategies for B-cell receptor repertoire analysis in primary immunodeficiencies: from severe combined immunodeficiency to common variable immunodeficiency","volume":"6","author":"IJspeert","year":"2015","journal-title":"Front Immunol"},{"key":"2023092815303308500_bib44","doi-asserted-by":"crossref","first-page":"2545","DOI":"10.3389\/fimmu.2018.02545","article-title":"Identification of CVID patients with defects in immune repertoire formation or specification","volume":"9","author":"van Schouwenburg","year":"2018","journal-title":"Front Immunol"},{"issue":"12","key":"2023092815303308500_bib45","doi-asserted-by":"crossref","first-page":"2257","DOI":"10.1038\/sj.leu.2403202","article-title":"Design and standardization of PCR primers and protocols for detection of clonal immunoglobulin and T-cell receptor gene recombinations in suspect lymphoproliferations: report of the BIOMED-2 Concerted Action BMH4-CT98-3936","volume":"17","author":"van Dongen","year":"2003","journal-title":"Leukemia"},{"issue":"5","key":"2023092815303308500_bib46","doi-asserted-by":"crossref","first-page":"790","DOI":"10.1101\/gr.115428.110","article-title":"Exhaustive T-cell repertoire sequencing of human peripheral blood samples reveals signatures of antigen selection and a directly measured repertoire size of at least 1 million clonotypes","volume":"21","author":"Warren","year":"2011","journal-title":"Genome Res"},{"issue":"10","key":"2023092815303308500_bib47","doi-asserted-by":"crossref","first-page":"4156","DOI":"10.4049\/jimmunol.1601921","article-title":"antigen receptor galaxy: a user-friendly, web-based tool for analysis and visualization of T and B cell receptor repertoire data","volume":"198","author":"IJspeert","year":"2017","journal-title":"J\u00a0Immunol"},{"key":"2023092815303308500_bib48","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1007\/978-1-61779-842-9_32","article-title":"IMGT(\u00ae) tools for the nucleotide analysis of immunoglobulin (IG) and T cell receptor (TR) V-(D)-J repertoires, polymorphisms, and IG mutations: IMGT\/V-QUEST and IMGT\/HighV-QUEST for NGS","volume":"882","author":"Alamyar","year":"2012","journal-title":"Methods Mol Biol"},{"issue":"12","key":"2023092815303308500_bib49","doi-asserted-by":"crossref","first-page":"12ra23","DOI":"10.1126\/scitranslmed.3000540","article-title":"Measurement and clinical monitoring of human lymphocyte clonality by massively parallel VDJ pyrosequencing","volume":"1","author":"Boyd","year":"2009","journal-title":"Sci Transl Med"},{"issue":"3","key":"2023092815303308500_bib50","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1038\/ni.2210","article-title":"A\u00a0self-reinforcing regulatory network triggered by limiting IL-7 activates pre-BCR signaling and differentiation","volume":"13","author":"Ochiai","year":"2012","journal-title":"Nat Immunol"},{"issue":"12","key":"2023092815303308500_bib51","doi-asserted-by":"crossref","first-page":"1171","DOI":"10.1038\/ni.3024","article-title":"The transcription repressors Bach2 and Bach1 promote B cell development by repressing the myeloid program","volume":"15","author":"Itoh-Nakadai","year":"2014","journal-title":"Nat Immunol"},{"issue":"14","key":"2023092815303308500_bib52","doi-asserted-by":"crossref","first-page":"3130","DOI":"10.1038\/emboj.2012.155","article-title":"The B-cell identity factor Pax5 regulates distinct transcriptional programmes in early and late B lymphopoiesis","volume":"31","author":"Revilla-I-Domingo","year":"2012","journal-title":"EMBO J"},{"issue":"2","key":"2023092815303308500_bib53","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1101\/gad.309583.117","article-title":"Dynamic EBF1 occupancy directs sequential epigenetic and transcriptional events in B-cell programming","volume":"32","author":"Li","year":"2018","journal-title":"Genes Dev"},{"issue":"75","key":"2023092815303308500_bib54","doi-asserted-by":"crossref","first-page":"eabo3170","DOI":"10.1126\/sciimmunol.abo3170","article-title":"Dysregulated stem cell niches and altered lymphocyte recirculation cause B and T cell lymphopenia in WHIM syndrome","volume":"7","author":"Zehentmeier","year":"2022","journal-title":"Sci Immunol"},{"issue":"4","key":"2023092815303308500_bib55","doi-asserted-by":"crossref","first-page":"e202301924","DOI":"10.26508\/lsa.202301924","article-title":"Mature B cells and mesenchymal stem cells control emergency myelopoiesis","volume":"6","author":"Lim","year":"2023","journal-title":"Life Sci Alliance"},{"issue":"18","key":"2023092815303308500_bib56","doi-asserted-by":"crossref","first-page":"5714","DOI":"10.1073\/pnas.80.18.5714","article-title":"Isolation of cDNA clones for the p33 invariant chain associated with HLA-DR antigens","volume":"80","author":"Long","year":"1983","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"24","key":"2023092815303308500_bib57","doi-asserted-by":"crossref","first-page":"7395","DOI":"10.1073\/pnas.80.24.7395","article-title":"cDNA clone for the human invariant gamma chain of class II histocompatibility antigens and its implications for the protein structure","volume":"80","author":"Claesson","year":"1983","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"11","key":"2023092815303308500_bib58","doi-asserted-by":"crossref","first-page":"1467","DOI":"10.1084\/jem.20030286","article-title":"MIF signal transduction initiated by binding to CD74","volume":"197","author":"Leng","year":"2003","journal-title":"J\u00a0Exp Med"},{"issue":"11","key":"2023092815303308500_bib59","doi-asserted-by":"crossref","first-page":"5273","DOI":"10.4049\/jimmunol.1302209","article-title":"MIF promotes B cell chemotaxis through the receptors CXCR4 and CD74 and ZAP-70 signaling","volume":"192","author":"Klasen","year":"2014","journal-title":"J\u00a0Immunol"},{"issue":"5284","key":"2023092815303308500_bib60","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1126\/science.274.5284.106","article-title":"Requirement for invariant chain in B cell maturation and function","volume":"274","author":"Shachar","year":"1996","journal-title":"Science"},{"issue":"5","key":"2023092815303308500_bib61","doi-asserted-by":"crossref","first-page":"2784","DOI":"10.1074\/jbc.M703265200","article-title":"Macrophage migration inhibitory factor induces B cell survival by activation of a CD74-CD44 receptor complex","volume":"283","author":"Gore","year":"2008","journal-title":"J\u00a0Biol Chem"},{"issue":"14","key":"2023092815303308500_bib62","doi-asserted-by":"crossref","first-page":"1132","DOI":"10.2174\/1389557515666150203144111","article-title":"The integral role of CD74 in antigen presentation, MIF signal transduction, and B cell survival and homeostasis","volume":"14","author":"Bucala","year":"2014","journal-title":"Mini Rev Med Chem"},{"issue":"10","key":"2023092815303308500_bib63","doi-asserted-by":"crossref","first-page":"2586","DOI":"10.1084\/jem.20180778","article-title":"Cell circuits between B cell progenitors and IL-7+ mesenchymal progenitor cells control B cell development","volume":"215","author":"Fistonich","year":"2018","journal-title":"J\u00a0Exp Med"},{"issue":"2","key":"2023092815303308500_bib64","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1016\/S0955-0674(97)80068-3","article-title":"AP-1 function and regulation","volume":"9","author":"Karin","year":"1997","journal-title":"Curr Opin Cell Biol"},{"issue":"3","key":"2023092815303308500_bib65","first-page":"685","article-title":"AP-1 (Fos\/Jun) transcription factors in hematopoietic differentiation and apoptosis","volume":"12","author":"Liebermann","year":"1998","journal-title":"Int J Oncol"},{"issue":"7","key":"2023092815303308500_bib66","doi-asserted-by":"crossref","first-page":"2059","DOI":"10.1084\/jem.20160514","article-title":"Fra-2 regulates B cell development by enhancing IRF4 and Foxo1 transcription","volume":"214","author":"Ubieta","year":"2017","journal-title":"J\u00a0Exp Med"},{"issue":"2","key":"2023092815303308500_bib67","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1111\/j.0019-2805.2003.01791.x","article-title":"Terminal deoxynucleotidyl transferase is down-regulated by AP-1-like regulatory elements in human lymphoid cells","volume":"111","author":"Peralta-Zaragoza","year":"2004","journal-title":"Immunology"},{"issue":"6537","key":"2023092815303308500_bib68","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1038\/376263a0","article-title":"Failure of B-cell differentiation in mice lacking the transcription factor EBF","volume":"376","author":"Lin","year":"1995","journal-title":"Nature"},{"issue":"6753","key":"2023092815303308500_bib69","doi-asserted-by":"crossref","first-page":"556","DOI":"10.1038\/44076","article-title":"Commitment to the B-lymphoid lineage depends on the transcription factor Pax5","volume":"401","author":"Nutt","year":"1999","journal-title":"Nature"},{"issue":"6","key":"2023092815303308500_bib70","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1016\/j.immuni.2007.05.010","article-title":"The transcriptional regulation of B cell lineage commitment","volume":"26","author":"Nutt","year":"2007","journal-title":"Immunity"},{"key":"2023092815303308500_bib71","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1146\/annurev-immunol-032712-100024","article-title":"Transcriptional control of early T and B cell developmental choices","volume":"32","author":"Rothenberg","year":"2014","journal-title":"Annu Rev Immunol"},{"issue":"6","key":"2023092815303308500_bib72","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1084\/jem.20042393","article-title":"Interleukin-7 is necessary to maintain the B cell potential in common lymphoid progenitors","volume":"201","author":"Dias","year":"2005","journal-title":"J\u00a0Exp Med"},{"issue":"17","key":"2023092815303308500_bib73","doi-asserted-by":"crossref","first-page":"2749","DOI":"10.1016\/j.febslet.2009.07.058","article-title":"A\u00a0functional heteromeric MIF receptor formed by CD74 and CXCR4","volume":"583","author":"Schwartz","year":"2009","journal-title":"FEBS Lett"},{"issue":"1","key":"2023092815303308500_bib74","doi-asserted-by":"crossref","first-page":"6843","DOI":"10.1038\/s41467-021-27232-5","article-title":"Single-cell analysis identifies dynamic gene expression networks that govern B cell development and transformation","volume":"12","author":"Lee","year":"2021","journal-title":"Nat Commun"},{"issue":"7","key":"2023092815303308500_bib75","doi-asserted-by":"crossref","first-page":"1824","DOI":"10.1002\/eji.200323974","article-title":"EBF-regulating Pax5 transcription is enhanced by STAT5 in the early stage of B cells","volume":"33","author":"Hirokawa","year":"2003","journal-title":"Eur J Immunol"},{"issue":"2","key":"2023092815303308500_bib76","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1128\/MCB.01192-06","article-title":"Distinct promoters mediate the regulation of Ebf1 gene expression by interleukin-7 and Pax5","volume":"27","author":"Roessler","year":"2007","journal-title":"Mol Cell Biol"},{"issue":"4","key":"2023092815303308500_bib77","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1016\/j.immuni.2009.01.012","article-title":"Stepwise activation of enhancer and promoter regions of the B cell commitment gene Pax5 in early lymphopoiesis","volume":"30","author":"Decker","year":"2009","journal-title":"Immunity"},{"issue":"7","key":"2023092815303308500_bib78","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1038\/ni.3441","article-title":"BACH2 regulates CD8+ T cell differentiation by controlling access of AP-1 factors to enhancers","volume":"17","author":"Roychoudhuri","year":"2016","journal-title":"Nat Immunol"},{"issue":"6","key":"2023092815303308500_bib79","doi-asserted-by":"crossref","first-page":"1067","DOI":"10.1016\/j.molcel.2017.11.026","article-title":"AP-1 transcription factors and the BAF complex mediate signal-dependent enhancer selection","volume":"68","author":"Vierbuchen","year":"2017","journal-title":"Mol Cell"},{"issue":"9","key":"2023092815303308500_bib80","doi-asserted-by":"crossref","first-page":"2052","DOI":"10.1038\/s41409-021-01378-8","article-title":"EBMT\/ESID inborn errors working party guidelines for hematopoietic stem cell transplantation for inborn errors of immunity","volume":"56","author":"Lankester","year":"2021","journal-title":"Bone Marrow Transplant"}],"container-title":["Blood"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/ashpublications.org\/blood\/article-pdf\/142\/13\/1113\/2081981\/blood_bld-2023-019721-main.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/ashpublications.org\/blood\/article-pdf\/142\/13\/1113\/2081981\/blood_bld-2023-019721-main.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,28]],"date-time":"2023-09-28T15:32:06Z","timestamp":1695915126000},"score":1,"resource":{"primary":{"URL":"https:\/\/ashpublications.org\/blood\/article\/142\/13\/1113\/496603\/IL-7-receptor-signaling-drives-human-B-cell"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9,28]]},"references-count":80,"journal-issue":{"issue":"13","published-print":{"date-parts":[[2023,9,28]]}},"URL":"https:\/\/doi.org\/10.1182\/blood.2023019721","relation":{},"ISSN":["0006-4971","1528-0020"],"issn-type":[{"value":"0006-4971","type":"print"},{"value":"1528-0020","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,9,28]]}}}