{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T16:03:18Z","timestamp":1793376198575,"version":"4.5.0"},"reference-count":13,"publisher":"American Society of Hematology","issue":"3","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2024,1,18]]},"abstract":"<jats:sec><jats:title>Abstract<\/jats:title><jats:p>Megakaryocytes (MKs) generate thousands of platelets over their lifespan. The roles of platelets in infection and inflammation has guided an interest to the study of extramedullary thrombopoiesis and therefore MKs have been increasingly reported within the spleen and lung. However, the relative abundance of MKs in these organs compared to the bone marrow and the scale of their contribution to the platelet pool in a steady state remain controversial. We investigated the relative abundance of MKs in the adult murine bone marrow, spleen, and lung using whole-mount light-sheet and quantitative histological imaging, flow cytometry, intravital imaging, and an assessment of single-cell RNA sequencing (scRNA-seq) repositories. Flow cytometry revealed significantly higher numbers of hematopoietic stem and progenitor cells and MKs in the murine bone marrow than in spleens or perfused lungs. Two-photon intravital and light-sheet microscopy, as well as quantitative histological imaging, confirmed these findings. Moreover, ex\u00a0vivo cultured MKs from the bone marrow subjected to static or microfluidic platelet production assays had a higher capacity for proplatelet formation than MKs from other organs. Analysis of previously published murine and human scRNA-seq data sets revealed that only a marginal fraction of MK-like cells can be found within the lung and most likely only marginally contribute to platelet production in the steady state.<\/jats:p><\/jats:sec>","DOI":"10.1182\/blood.2023020895","type":"journal-article","created":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T20:01:14Z","timestamp":1698264074000},"page":"272-278","update-policy":"https:\/\/doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":69,"title":["The bone marrow is the primary site of thrombopoiesis"],"prefix":"10.1182","volume":"143","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7073-825X","authenticated-orcid":false,"given":"Nathan L.","family":"Asquith","sequence":"first","affiliation":[{"name":"1Vascular Biology Program, Department of Surgery, Boston Children's Hospital, Boston, MA"},{"name":"2Harvard Medical School, Boston, MA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8544-4879","authenticated-orcid":false,"given":"Estelle","family":"Carminita","sequence":"additional","affiliation":[{"name":"1Vascular Biology Program, Department of Surgery, Boston Children's Hospital, Boston, MA"},{"name":"2Harvard Medical School, Boston, MA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6970-0932","authenticated-orcid":false,"given":"Virginia","family":"Camacho","sequence":"additional","affiliation":[{"name":"1Vascular Biology Program, Department of Surgery, Boston Children's Hospital, Boston, MA"},{"name":"2Harvard Medical School, Boston, MA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7193-9144","authenticated-orcid":false,"given":"Antonio","family":"Rodriguez-Romera","sequence":"additional","affiliation":[{"name":"3MRC Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, and National Institute of Health Research Oxford Biomedical Research Centre, University of Oxford, Oxford, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1059-9865","authenticated-orcid":false,"given":"David","family":"Stegner","sequence":"additional","affiliation":[{"name":"4Rudolf Virchow Center for Integrative and Translational Bioimaging, Julius-Maximilians-Universit\u00e4t W\u00fcrzburg, W\u00fcrzburg, Germany"},{"name":"5Institute of Experimental Biomedicine, University Hospital W\u00fcrzburg, W\u00fcrzburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7861-0218","authenticated-orcid":false,"given":"Daniela","family":"Freire","sequence":"additional","affiliation":[{"name":"1Vascular Biology Program, Department of Surgery, Boston Children's Hospital, Boston, MA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2725-8493","authenticated-orcid":false,"given":"Isabelle C.","family":"Becker","sequence":"additional","affiliation":[{"name":"1Vascular Biology Program, Department of Surgery, Boston Children's Hospital, Boston, MA"},{"name":"2Harvard Medical School, Boston, MA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kellie R.","family":"Machlus","sequence":"additional","affiliation":[{"name":"1Vascular Biology Program, Department of Surgery, Boston Children's Hospital, Boston, MA"},{"name":"2Harvard Medical School, Boston, MA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdullah O.","family":"Khan","sequence":"additional","affiliation":[{"name":"1Vascular Biology Program, Department of Surgery, Boston Children's Hospital, Boston, MA"},{"name":"2Harvard Medical School, Boston, MA"},{"name":"3MRC Weatherall Institute of Molecular Medicine, Radcliffe Department 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