{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T03:46:14Z","timestamp":1792122374463,"version":"4.4.0"},"reference-count":85,"publisher":"American Society of Hematology","issue":"14","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2024,10,3]]},"abstract":"<jats:sec>\n                    <jats:title>Abstract<\/jats:title>\n                    <jats:p>Red blood cells (RBCs) have been hypothesized to support hemostasis by facilitating platelet margination and releasing platelet-activating factors such as adenosine 5\u2032-diphosphate (ADP). Significant knowledge gaps remain regarding how RBCs influence platelet function, especially in (patho)physiologically relevant hemodynamic conditions. Here, we present results showing how RBCs affect platelet function and hemostasis in conditions of anemia, thrombocytopenia, and pancytopenia and how the biochemical and biophysical properties of RBCs regulate platelet function at the blood and vessel wall interface and in the fluid phase under flow conditions. We found that RBCs promoted platelet deposition to collagen under flow conditions in moderate (50\u00a0\u00d7 103\/\u03bcL) but not severe (10\u00a0\u00d7 103\/\u03bcL) thrombocytopenia in\u00a0vitro. Reduction in hematocrit by 45% increased bleeding in mice with hemolytic anemia. In contrast, bleeding diathesis was observed in mice with a 90% but not with a 60% reduction in platelet counts. RBC transfusion improved hemostasis by enhancing fibrin clot formation at the site of vascular injury in mice with severe pancytopenia induced by total body irradiation. Altering membrane deformability changed the ability of RBCs to promote shear-induced platelet aggregation. RBC-derived ADP contributed to platelet activation and aggregation in\u00a0vitro under pathologically high shear stresses, as observed in patients supported by left ventricular assist devices. These findings demonstrate that RBCs support platelet function and hemostasis through multiple mechanisms, both at the blood and vessel wall interface and in the fluidic phase of circulation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1182\/blood.2024023887","type":"journal-article","created":{"date-parts":[[2024,7,10]],"date-time":"2024-07-10T13:43:56Z","timestamp":1720619036000},"page":"1521-1531","update-policy":"https:\/\/doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":36,"title":["RBCs regulate platelet function and hemostasis under shear conditions through biophysical and biochemical means"],"prefix":"10.1182","volume":"144","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2978-3585","authenticated-orcid":false,"given":"Debbie","family":"Jiang","sequence":"first","affiliation":[{"name":"1Division of Hematology and Oncology, University of Washington, Seattle, WA"},{"name":"2Fred Hutchinson Cancer Center, Seattle, WA"},{"name":"3Division of Hematology, Massachusetts General Hospital, Harvard Medical School, Boston, MA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katie L.","family":"Houck","sequence":"additional","affiliation":[{"name":"4Bloodworks Research Institute, Seattle, WA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lydia","family":"Murdiyarso","sequence":"additional","affiliation":[{"name":"4Bloodworks Research Institute, Seattle, WA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-4304-3972","authenticated-orcid":false,"given":"Harrison","family":"Higgins","sequence":"additional","affiliation":[{"name":"4Bloodworks Research Institute, Seattle, WA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8331-5136","authenticated-orcid":false,"given":"Nicole","family":"Rhoads","sequence":"additional","affiliation":[{"name":"4Bloodworks Research Institute, Seattle, WA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophia K.","family":"Romero","sequence":"additional","affiliation":[{"name":"4Bloodworks Research Institute, Seattle, WA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosemary","family":"Kozar","sequence":"additional","affiliation":[{"name":"5Department of Surgery, University of Maryland School of Medicine, Baltimore, MD"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angelo","family":"Nascimbene","sequence":"additional","affiliation":[{"name":"6Center for Advanced Cardiopulmonary Therapies and Transplantation, The University of Texas Houston Health Science Center, Houston, TX"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2671-5608","authenticated-orcid":false,"given":"Terry B.","family":"Gernsheimer","sequence":"additional","affiliation":[{"name":"1Division of Hematology and Oncology, University of Washington, Seattle, 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