{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T18:35:25Z","timestamp":1789756525171,"version":"4.0.1"},"reference-count":49,"publisher":"American Society of Hematology","issue":"19","license":[{"start":{"date-parts":[[2025,1,27]],"date-time":"2025-01-27T00:00:00Z","timestamp":1737936000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2025,5,8]]},"abstract":"<jats:sec>\n                  <jats:title>Abstract<\/jats:title>\n                  <jats:p>Babesiosis in sickle cell disease (SCD) is marked by severe anemia but the underlying red blood cell (RBC) rheologic parameters remain largely undefined. Here, we describe altered RBC deformability from both primary (host RBC sickle hemoglobin mediated) and secondary changes (Babesia parasite infection mediated) to the RBC membrane using wild-type AA, sickle trait AS, and sickle SS RBCs. Our ektacytometry analysis demonstrates that the changes in the host RBC biomechanical properties, before and after Babesia infection, reside on a spectrum of severity, with wild-type infected AA cells, despite showing a significant reduction of deformability under both shear and osmolarity gradients, exhibiting only a mild phenotype, compared with infected AS RBCs that show median changes in deformability and infected SS RBCs that exhibit the most dramatic impact of infection on cellular rheology, including an increase in point of sickling values. Furthermore, using ImageStream cytometric technology to quantify changes in cellular shape and area along with a tunable resistive pulse sensor to measure release of extracellular vesicles from these host RBCs, before and after infection, we offer a potential mechanistic basis for this extreme SS RBC rheologic profile, which include enhanced sickling rates and altered osmotic fragility, loss of RBC surface area, and hypervesiculation in infected SS host RBCs. These results underline the importance of understanding the impact of intraerythrocytic parasitic infections of SCD RBCs, especially on their cellular membranes and studying the mechanisms that lead to hyperhemolysis and extreme anemia in patients with SCD.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1182\/blood.2024027602","type":"journal-article","created":{"date-parts":[[2025,1,27]],"date-time":"2025-01-27T20:58:36Z","timestamp":1738011516000},"page":"2202-2213","update-policy":"https:\/\/doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":5,"title":["Babesiosis and sickle red blood cells: loss of deformability, altered osmotic fragility, and hypervesiculation"],"prefix":"10.1182","volume":"145","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5966-419X","authenticated-orcid":false,"given":"Divya","family":"Beri","sequence":"first","affiliation":[{"name":"1Department of Blood-Borne Parasites, Lindsley F. Kimball Research Institute, New York Blood Center, New York, NY"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marilis","family":"Rodriguez","sequence":"additional","affiliation":[{"name":"1Department of Blood-Borne Parasites, Lindsley F. Kimball Research Institute, New York Blood Center, New York, NY"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manpreet","family":"Singh","sequence":"additional","affiliation":[{"name":"1Department of Blood-Borne Parasites, Lindsley F. Kimball Research Institute, New York Blood Center, New York, NY"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1806-6943","authenticated-orcid":false,"given":"Daniel","family":"McLaughlin","sequence":"additional","affiliation":[{"name":"2iPSC Project Operations, Lindsley F. Kimball Research Institute, New York Blood Center, New York, NY"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9187-4146","authenticated-orcid":false,"given":"Yunfeng","family":"Liu","sequence":"additional","affiliation":[{"name":"3Department of Complement Biology, Lindsley F. Kimball Research Institute, New York Blood Center, New York, NY"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Zhong","sequence":"additional","affiliation":[{"name":"4Department of Immune Regulation, Lindsley F. Kimball Research Institute, New York Blood Center, New York, NY"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1120-9593","authenticated-orcid":false,"given":"Avital","family":"Mendelson","sequence":"additional","affiliation":[{"name":"5Laboratory of Stem Cell Biology and Engineering Research, Lindsley F. Kimball Research Institute, New York Blood Center, New York, NY"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuli","family":"An","sequence":"additional","affiliation":[{"name":"6Department of Membrane Biology, Lindsley F. Kimball Research Institute, New York Blood Center, New York, NY"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deepa","family":"Manwani","sequence":"additional","affiliation":[{"name":"7Division of Hematology, Department of Medicine, Montefiore Health Center, Albert Einstein College of Medicine, Bronx, NY"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karina","family":"Yazdanbakhsh","sequence":"additional","affiliation":[{"name":"3Department of Complement Biology, Lindsley F. 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