{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,6]],"date-time":"2026-11-06T04:33:59Z","timestamp":1793939639406,"version":"4.8.0"},"reference-count":40,"publisher":"American Society of Hematology","issue":"19","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2026,11,5]]},"abstract":"<jats:sec>\n                    <jats:title>Abstract<\/jats:title>\n                    <jats:p>Leukocyte adhesion deficiency syndrome type III is characterized by recurrent infections and is caused by FERMT3 gene (encoding kindlin-3) mutations. FERMT3 encodes 2 isoforms, a standard kindlin-3 and a longer splicing variant, which differs by the addition of 4 residues isoleucine-proline-arginine-arginine (IPRR) in the pleckstrin homology (PH) domain (kindlin-3\u2013IPRR). Previous studies suggested that kindlin-3\u2013IPRR was dysfunctional in inducing neutrophil adhesion, attributed to altered phospholipid binding of the IPRR-containing PH domain. Here, we show that kindlin-3\u2013IPRR is fully functional in activating \u03b22 integrins and promotes neutrophil adhesion. The IPRR insert enhances phospholipid binding in vitro and promotes association of kindlin-3 with the plasma membrane. By analyzing RNA-sequencing data sets of neutropoiesis, we show that the kindlin-3 short isoform lacking IPRR is highly expressed in neutrophil precursors but downregulated in mature neutrophils. In contrast, a higher proportion of the long isoform was detected in hematopoietic stem cells (HSCs) and mature neutrophils. Kindlin-3\u2013IPRR supports robust adhesion of HSCs. We propose that kindlin-3\u2013IPRR may be crucial in conditions requiring rapid neutrophil mobilization. Indeed, kindlin-3 splicing is altered in neutrophils from patients undergoing stress myelopoiesis after exposure to granulocyte colony-stimulating factor (G-CSF) or HSC transplantation (HSCT) compared with healthy donors. During stress myelopoiesis induced by G-CSF treatment, the kindlin-3 long isoform is selectively upregulated in mature neutrophils, coinciding with enhanced \u03b22-integrin activation. These findings support a model in which alternative splicing of FERMT3 dynamically fine-tunes integrin activation and leukocyte adhesion during hematopoietic stress, with potential implications for monitoring hematopoietic recovery after HSCT.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1182\/blood.2026034274","type":"journal-article","created":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T17:10:26Z","timestamp":1786381826000},"page":"2399-2410","update-policy":"https:\/\/doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":1,"title":["FERMT3 alternative splicing enhances kindlin-3 membrane recruitment for neutrophil adhesion during stress myelopoiesis"],"prefix":"10.1182","volume":"148","author":[{"given":"Serena","family":"Lee","sequence":"first","affiliation":[{"name":"1Center for Molecular and Cellular Signaling in the Cardiovascular System, Department of Pharmacology, School of Medicine, University of Nevada, Reno, Reno, 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