{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,4]],"date-time":"2025-10-04T12:09:54Z","timestamp":1759579794541},"reference-count":46,"publisher":"American Society of Hematology","issue":"10","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2002,11,15]]},"abstract":"<jats:p>To define the phosphatidylinositol glycan-class A (PIG-A) gene abnormality in precursor cells and the changes of expression of glycosylphosphatidylinositol-anchored protein and contribution of paroxysmal nocturnal hemoglobinuria (PNH) clones with PIG-A gene abnormalities among various cell lineages during differentiation and maturation, we investigated CD59 expression on bone marrow CD34+ cells and peripheral granulocytes from 3 patients with PNH and the PIG-A gene abnormalities in the CD59\u2212, CD59+\/\u2212, and CD59+ populations by nucleotide sequence analyses. We also performed clonogeneic assays of CD34+CD59+ and CD34+CD59\u2212 cells from 2 of the patients and examined the PIG-A gene abnormalities in the cultured cells. In case 1, the CD34+ cells and granulocytes consisted of CD59\u2212 and CD59+ populations and CD59\u2212, CD59+\/\u2212, and CD59+populations, respectively. Sequence analyses indicated that mutation 1-2 was in the CD59+\/\u2212 granulocyte population (20 of 20) and the CD34+CD59\u2212 population (2 of 38). In cases 2 and 3, the CD34+ cells and granulocytes consisted of CD59+ and CD59\u2212 cells. Sequence analyses in case 3 showed that mutation 3-2 was not in CD34+CD59\u2212 cells and was present in the CD59\u2212 granulocyte population. However, PIG-A gene analysis of cultured CD34+CD59\u2212 cells showed that they had the mutation. This analysis also revealed that there were some other mutations, which were not found in CD34+CD59\u2212 cells and CD59\u2212 or CD59+\/\u2212 granulocytes in vivo, and that sometimes they were distributed specifically among different cell lineages. In conclusion, our findings suggest that PNH clones might contribute qualitatively and quantitatively differentially to specific blood cell lineages during differentiation and maturation of hematopoietic stem cells.<\/jats:p>","DOI":"10.1182\/blood.v100.10.3812","type":"journal-article","created":{"date-parts":[[2002,10,30]],"date-time":"2002-10-30T19:43:16Z","timestamp":1036006996000},"page":"3812-3818","update-policy":"http:\/\/dx.doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":8,"title":["Phenotypes and phosphatidylinositol glycan-class A gene abnormalities during cell differentiation and maturation from precursor cells to mature granulocytes in patients with paroxysmal nocturnal hemoglobinuria"],"prefix":"10.1182","volume":"100","author":[{"given":"Tatsuyuki","family":"Kai","sequence":"first","affiliation":[{"name":"From the First Department of Internal Medicine, Fukushima Medical University, Fukushima, Japan, and the Third Department of Internal 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Internal Medicine, National Defense Medical College, Saitama, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masatoshi","family":"Okamoto","sequence":"additional","affiliation":[{"name":"From the First Department of Internal Medicine, Fukushima Medical University, Fukushima, Japan, and the Third Department of Internal Medicine, National Defense Medical College, Saitama, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuhiko","family":"Ikeda","sequence":"additional","affiliation":[{"name":"From the First Department of Internal Medicine, Fukushima Medical University, Fukushima, Japan, and the Third Department of Internal Medicine, National Defense Medical College, Saitama, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yukio","family":"Maruyama","sequence":"additional","affiliation":[{"name":"From the First Department of Internal Medicine, Fukushima Medical University, Fukushima, Japan, and the Third Department 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