{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T15:04:56Z","timestamp":1770822296195,"version":"3.50.1"},"reference-count":23,"publisher":"American Society of Hematology","issue":"2","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2002,7,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Using immunohistochemistry, we investigated the expression of c-mpl in bone marrow megakaryocytes of 88 patients with essential thrombocythemia (ET), 6 patients with secondary thrombocytosis (ST), and 20 patients with lymphoma (controls). Considering both the pattern of expression and the staining intensity, we identified a uniform and a heterogeneous pattern of c-mplexpression. The uniform pattern was found in all the controls, all the patients with ST, and 28 of the patients with ET, with a strong staining intensity observed in most megakaryocytes (&amp;gt; 80%). In contrast, c-mpl expression was heterogeneous in 60 patients with ET, 18 of whom (30%) presented with thrombosis at diagnosis, a significant difference from patients with a uniform c-mpl pattern (2 of 28; 7%; P\u2009=\u2009.026). In particular, the overrepresentation of thrombotic complications in patients with a heterogeneous c-mpl expression pattern was found mainly among patients with a significant percentage (10% to 40%) of weakly stained or c-mpl\u2013negative megakaryocytes (heterogeneous-weak pattern; 13 of 30; 43%;P\u2009=\u2009.002). Accordingly, this pattern was associated with a 6.1-fold increased risk of thrombosis compared with that of patients with a uniform c-mpl pattern. In conclusion, the presence of a heterogeneous pattern of c-mpl distribution in bone marrow megakaryocytes could be a useful diagnostic criterion in the differential diagnosis of thrombocytosis. Furthermore, detection of a significant percentage of weakly stained or c-mpl\u2013negative megakaryocytes can identify patients with a higher risk of thrombosis.<\/jats:p>","DOI":"10.1182\/blood.v100.2.714","type":"journal-article","created":{"date-parts":[[2002,10,1]],"date-time":"2002-10-01T21:48:18Z","timestamp":1033508898000},"page":"714-717","update-policy":"https:\/\/doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":31,"title":["The expression pattern of c-mpl in megakaryocytes correlates with thrombotic risk in essential thrombocythemia"],"prefix":"10.1182","volume":"100","author":[{"given":"Luciana","family":"Teofili","sequence":"first","affiliation":[{"name":"From the Departments of Hematology and Pathology, Catholic University, Rome, Italy; and the Institute of Hematology Seragnoli, University of Bologna, Bologna, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesco","family":"Pierconti","sequence":"additional","affiliation":[{"name":"From the Departments of Hematology and Pathology, Catholic University, Rome, Italy; and the Institute of Hematology Seragnoli, University of Bologna, Bologna, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annalaura","family":"Di Febo","sequence":"additional","affiliation":[{"name":"From the Departments of Hematology and Pathology, Catholic University, Rome, Italy; and the Institute of Hematology Seragnoli, University of Bologna, Bologna, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicola","family":"Maggiano","sequence":"additional","affiliation":[{"name":"From the Departments of Hematology and Pathology, Catholic University, Rome, Italy; and the Institute of Hematology Seragnoli, University of Bologna, Bologna, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicola","family":"Vianelli","sequence":"additional","affiliation":[{"name":"From the Departments of Hematology and Pathology, Catholic University, Rome, Italy; and the Institute of Hematology Seragnoli, University of Bologna, Bologna, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefano","family":"Ascani","sequence":"additional","affiliation":[{"name":"From the Departments of Hematology and Pathology, Catholic University, Rome, Italy; and the Institute of Hematology Seragnoli, University of Bologna, Bologna, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Rossi","sequence":"additional","affiliation":[{"name":"From the Departments of Hematology and Pathology, Catholic University, Rome, Italy; and the Institute of Hematology Seragnoli, University of Bologna, Bologna, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefano","family":"Pileri","sequence":"additional","affiliation":[{"name":"From the Departments of Hematology and Pathology, Catholic University, Rome, Italy; and the Institute of Hematology Seragnoli, University of Bologna, Bologna, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giuseppe","family":"Leone","sequence":"additional","affiliation":[{"name":"From the Departments of Hematology and Pathology, Catholic University, Rome, Italy; and the Institute of Hematology Seragnoli, University of Bologna, Bologna, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luigi Maria","family":"Larocca","sequence":"additional","affiliation":[{"name":"From the Departments of Hematology and Pathology, Catholic University, Rome, Italy; and the Institute of Hematology Seragnoli, University of Bologna, Bologna, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valerio","family":"De Stefano","sequence":"additional","affiliation":[{"name":"From the Departments of Hematology and Pathology, Catholic University, Rome, Italy; and the Institute of Hematology Seragnoli, University of Bologna, Bologna, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"234","reference":[{"key":"2020021220222169400_B1","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1055\/s-0038-1642732","article-title":"Thrombopoietin: the primary regulator of megakaryocyte and platelet production.","volume":"74","author":"Kaushansky","year":"1995","journal-title":"Thromb Haemost."},{"key":"2020021220222169400_B2","doi-asserted-by":"crossref","first-page":"3234","DOI":"10.1073\/pnas.92.8.3234","article-title":"Thrombopoietin, the Mp1 ligand, is essential for full megakaryocyte development.","volume":"92","author":"Kaushansky","year":"1995","journal-title":"Proc Natl Acad Sci U S 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