{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T05:34:17Z","timestamp":1791178457891,"version":"4.1.0"},"reference-count":48,"publisher":"American Society of Hematology","issue":"3","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2002,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Vascular endothelial cadherin (VE-cadherin) is an endothelial-specific, trans-membrane protein that promotes homophilic cell adhesion. Inhibition of VE-cadherin by the blocking monoclonal antibody (mAb) BV13 inhibited angiogenesis and tumor growth in vivo. However, this effect was accompanied by a marked increase in lung and heart permeability. In the present paper, we characterize a different VE-cadherin mAb (BV14) that is able to inhibit angiogenesis without affecting vascular permeability. In vitro studies show that BV14, in contrast to BV13, did not increase paracellular permeability of endothelial monolayers and did not disrupt VE-cadherin clusters at junctions. However, both antibodies could inhibit formation of vascularlike structures in collagen gels and increase migration of endothelial cells into wounded areas. In vivo, BV14 and BV13 were equally active in inhibiting angiogenesis in the mouse cornea and in reducing the growth of hemangioma and C6 glioma. In contrast to BV13, BV14 did not change vascular permeability in all the organs tested and at any dose used. BV14 and BV13 bind to VE-cadherin extracellular repeats EC4 and EC1, respectively. We propose that, in resting vessels, where junctions are stable and well-structured, antibody binding to EC1 but not EC4 disrupts their organization and increases permeability. In contrast, in growing vessels, where endothelial cells are migrating and junctions are weaker, antibody binding to EC4 may be sufficient to disrupt cell-to-cell adhesion and inhibit assembly of new vascular structures.<\/jats:p>","DOI":"10.1182\/blood.v100.3.905","type":"journal-article","created":{"date-parts":[[2002,10,11]],"date-time":"2002-10-11T18:33:52Z","timestamp":1034361232000},"page":"905-911","update-policy":"https:\/\/doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":171,"title":["A monoclonal antibody to vascular endothelial\u2013cadherin inhibits tumor angiogenesis without side effects on endothelial permeability"],"prefix":"10.1182","volume":"100","author":[{"given":"Monica","family":"Corada","sequence":"first","affiliation":[{"name":"From the FIRC Institute of Molecular Oncology, Milan, Italy; Mario Negri Institute for Pharmacological Research, Milan, Italy; Universita\u0301 degli Studi dell'Insubria, Dipartimento di Scienze Cliniche e Biologiche, Facolta\u0301 di Medicina e Chirurgia, Varese, Italy; Department of Immunology, ImClone Systems Incorporated, New York, NY."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucia","family":"Zanetta","sequence":"additional","affiliation":[{"name":"From the FIRC Institute of Molecular Oncology, Milan, Italy; Mario Negri Institute for Pharmacological Research, Milan, Italy; Universita\u0301 degli Studi dell'Insubria, Dipartimento di Scienze Cliniche e Biologiche, Facolta\u0301 di Medicina e Chirurgia, Varese, Italy; Department of Immunology, ImClone Systems Incorporated, New York, NY."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabrizio","family":"Orsenigo","sequence":"additional","affiliation":[{"name":"From the FIRC Institute of Molecular Oncology, Milan, Italy; Mario Negri Institute for Pharmacological Research, Milan, Italy; Universita\u0301 degli Studi dell'Insubria, Dipartimento di Scienze Cliniche e Biologiche, Facolta\u0301 di Medicina e Chirurgia, Varese, Italy; Department of Immunology, ImClone Systems Incorporated, New York, NY."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ferruccio","family":"Breviario","sequence":"additional","affiliation":[{"name":"From the FIRC Institute of Molecular Oncology, Milan, Italy; Mario Negri Institute for Pharmacological Research, Milan, Italy; Universita\u0301 degli Studi dell'Insubria, Dipartimento di Scienze Cliniche e Biologiche, Facolta\u0301 di Medicina e Chirurgia, Varese, Italy; Department of Immunology, ImClone Systems Incorporated, New York, NY."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Grazia","family":"Lampugnani","sequence":"additional","affiliation":[{"name":"From the FIRC Institute of Molecular Oncology, Milan, Italy; Mario Negri Institute for Pharmacological Research, Milan, Italy; Universita\u0301 degli Studi dell'Insubria, Dipartimento di Scienze Cliniche e Biologiche, Facolta\u0301 di Medicina e Chirurgia, Varese, Italy; Department of Immunology, ImClone Systems Incorporated, New York, NY."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio","family":"Bernasconi","sequence":"additional","affiliation":[{"name":"From the FIRC Institute of Molecular Oncology, Milan, Italy; Mario Negri Institute for Pharmacological Research, Milan, Italy; Universita\u0301 degli Studi dell'Insubria, Dipartimento di Scienze Cliniche e Biologiche, Facolta\u0301 di Medicina e Chirurgia, Varese, Italy; Department of Immunology, ImClone Systems Incorporated, New York, NY."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Liao","sequence":"additional","affiliation":[{"name":"From the FIRC Institute of Molecular Oncology, Milan, Italy; Mario Negri Institute for Pharmacological Research, Milan, Italy; Universita\u0301 degli Studi dell'Insubria, Dipartimento di Scienze Cliniche e Biologiche, Facolta\u0301 di Medicina e Chirurgia, Varese, Italy; Department of Immunology, ImClone Systems Incorporated, New York, NY."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel J.","family":"Hicklin","sequence":"additional","affiliation":[{"name":"From the FIRC Institute of Molecular Oncology, Milan, Italy; Mario Negri Institute for Pharmacological Research, Milan, Italy; Universita\u0301 degli Studi dell'Insubria, Dipartimento di Scienze Cliniche e Biologiche, Facolta\u0301 di Medicina e Chirurgia, Varese, Italy; Department of Immunology, ImClone Systems Incorporated, New York, NY."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Bohlen","sequence":"additional","affiliation":[{"name":"From the FIRC Institute of Molecular Oncology, Milan, Italy; Mario Negri Institute for Pharmacological Research, Milan, Italy; Universita\u0301 degli Studi dell'Insubria, Dipartimento di Scienze Cliniche e Biologiche, Facolta\u0301 di Medicina e Chirurgia, Varese, Italy; Department of Immunology, ImClone Systems Incorporated, New York, NY."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabetta","family":"Dejana","sequence":"additional","affiliation":[{"name":"From the FIRC Institute of Molecular Oncology, Milan, Italy; Mario Negri Institute for Pharmacological Research, Milan, Italy; Universita\u0301 degli Studi dell'Insubria, Dipartimento di Scienze Cliniche e Biologiche, Facolta\u0301 di Medicina e Chirurgia, Varese, Italy; Department of Immunology, ImClone Systems Incorporated, New York, NY."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"234","reference":[{"key":"2020021221262949200_B1","doi-asserted-by":"crossref","first-page":"1949","DOI":"10.1172\/JCI118997","article-title":"Endothelial adherens junctions: implications in the control of vascular permeability and angiogenesis.","volume":"98","author":"Dejana","year":"1996","journal-title":"J Clin 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