{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T05:49:06Z","timestamp":1790920146931,"version":"4.1.0"},"reference-count":28,"publisher":"American Society of Hematology","issue":"9","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2002,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Autoimmune phenomena may precede or accompany lymphoid malignancies, especially B-chronic lymphocytic leukemia (B-CLL). We report a patient with a 7-year history of primary (idiopathic) cold agglutinin (CA) disease in whom B-CLL subsequently developed. Immunophenotyping and single-cell reverse transcription\u2013polymerase chain reaction (RT-PCR) were applied to investigate the origin and diversification of leukemic B cells. The obtained data indicate a memory cell-type origin of the B-CLL cells. Remarkably, theIgV\u03ba genes of the B-CLL cells showed intraclonal diversity, whereas the mutational pattern of their paired IgVH genes were invariant. Thus, the light-chain\u2013restricted intraclonal diversity in individual leukemic B cells in this patient strongly indicates a differential regulation or selection of the ongoing mutational process. Of note, our findings suggest that this B-CLL had developed from the patient's CA-producing B-cell population.<\/jats:p>","DOI":"10.1182\/blood.v100.9.3419","type":"journal-article","created":{"date-parts":[[2002,10,16]],"date-time":"2002-10-16T23:03:08Z","timestamp":1034809388000},"page":"3419-3422","update-policy":"https:\/\/doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":36,"title":["Chronic lymphocytic leukemia preceded by cold agglutinin disease: intraclonal immunoglobulin light-chain diversity inVH4-34 expressing single leukemic B cells"],"prefix":"10.1182","volume":"100","author":[{"given":"Sarka","family":"Ruzickova","sequence":"first","affiliation":[{"name":"From Deutsches Rheumaforschungszentrum, Berlin; Department of Transfusion Medicine\/Outpatients Department and Department of Rheumatology and Clinical Immunology, Charite\u0301University Hospital, Berlin, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Axel","family":"Pruss","sequence":"additional","affiliation":[{"name":"From Deutsches Rheumaforschungszentrum, Berlin; Department of Transfusion Medicine\/Outpatients Department and Department of Rheumatology and Clinical Immunology, Charite\u0301University Hospital, Berlin, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcus","family":"Odendahl","sequence":"additional","affiliation":[{"name":"From Deutsches Rheumaforschungszentrum, Berlin; Department of Transfusion Medicine\/Outpatients Department and Department of Rheumatology and Clinical Immunology, Charite\u0301University Hospital, Berlin, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karsten","family":"Wolbart","sequence":"additional","affiliation":[{"name":"From Deutsches Rheumaforschungszentrum, Berlin; Department of Transfusion Medicine\/Outpatients Department and Department of Rheumatology and Clinical Immunology, Charite\u0301University Hospital, Berlin, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerd-Ru\u0308diger","family":"Burmester","sequence":"additional","affiliation":[{"name":"From Deutsches Rheumaforschungszentrum, Berlin; Department of Transfusion Medicine\/Outpatients Department and Department of Rheumatology and Clinical Immunology, Charite\u0301University Hospital, Berlin, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ju\u0308rgen","family":"Scholze","sequence":"additional","affiliation":[{"name":"From Deutsches Rheumaforschungszentrum, Berlin; Department of Transfusion Medicine\/Outpatients Department and Department of Rheumatology and Clinical Immunology, Charite\u0301University Hospital, Berlin, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Do\u0308rner","sequence":"additional","affiliation":[{"name":"From Deutsches Rheumaforschungszentrum, Berlin; 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