{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T05:01:16Z","timestamp":1790744476304,"version":"4.1.0"},"reference-count":26,"publisher":"American Society of Hematology","issue":"3","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2003,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Chromosomal translocations involving t(14;18)(q32;q21) and the chromosome 3q27 region are common in B-cell non-Hodgkin lymphoma of germinal center cell origin. Grade 3B follicular lymphoma (FL), consisting almost exclusively of centroblasts, is a distinct subgroup of follicular lymphomas that has more in common clinically with the aggressive diffuse large B-cell lymphomas than with their indolent FL grade 1 and 2 counterparts. We studied the cytogenetic and molecular genetic aberrations by classic cytogenetics, polymerase chain reaction, Southern blot hybridization, and fluorescence in situ hybridization, with special emphasis on t(14;18), affecting bcl-2, and 3q27 rearrangement, affecting bcl-6, in 32 cases of FL grade 3B. Three distinctive subgroups were identified based upon the existence of breakpoint 3q27, a translocation t(14;18), or the absence of both. Group I involved a t(14;18) and no 3q27 aberrations (n\u2009=\u200913); group II was without a t(14;18) and without 3q27 aberrations (n\u2009=\u20099), but had other cytogenetic aberrations; and group III was without a t(14;18) but with aberrations involving 3q27 (n\u2009=\u200910). None of the FL grade 3B cases harbored both a t(14;18) and 3q27 aberration. These results, in particular the finding of a mutual exclusiveness of bcl-2 and bcl-6 rearrangement, indicate at least 3 different pathways of oncogenesis in FL grade 3B. FL grade 3B with bcl-2 rearrangement probably is part of the same entity as the other follicular lymphomas (1, 2, 3A), whereas the cases with 3q27 abnormalities or other unrelated translocations are more closely related to the majority of diffuse large-cell lymphomas of germinal center cell origin.<\/jats:p>","DOI":"10.1182\/blood.v101.3.1149","type":"journal-article","created":{"date-parts":[[2003,1,15]],"date-time":"2003-01-15T21:05:10Z","timestamp":1042664710000},"page":"1149-1154","update-policy":"https:\/\/doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":122,"title":["Follicular lymphoma grade 3B includes 3 cytogenetically defined subgroups with primary t(14;18), 3q27, or other translocations: t(14;18) and 3q27 are mutually exclusive"],"prefix":"10.1182","volume":"101","author":[{"given":"Anneke G.","family":"Bosga-Bouwer","sequence":"first","affiliation":[{"name":"From the Departments of Genetics, Hematology, and Pathology & Laboratory Medicine, University Medical Center Groningen, The Netherlands; and Cross Cancer Institute, Edmonton, AB, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustaaf W.","family":"van Imhoff","sequence":"additional","affiliation":[{"name":"From the Departments of Genetics, Hematology, and Pathology & Laboratory Medicine, University Medical Center Groningen, The Netherlands; and Cross Cancer Institute, Edmonton, AB, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronald","family":"Boonstra","sequence":"additional","affiliation":[{"name":"From the Departments of Genetics, Hematology, and Pathology & Laboratory Medicine, University Medical Center Groningen, The Netherlands; and Cross Cancer Institute, Edmonton, AB, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anneke","family":"van der Veen","sequence":"additional","affiliation":[{"name":"From the Departments of Genetics, Hematology, and Pathology & Laboratory Medicine, University Medical Center Groningen, The Netherlands; and Cross Cancer Institute, Edmonton, AB, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eugenia","family":"Haralambieva","sequence":"additional","affiliation":[{"name":"From the Departments of Genetics, Hematology, and Pathology & Laboratory Medicine, University Medical Center Groningen, The Netherlands; and Cross Cancer Institute, Edmonton, AB, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anke","family":"van den Berg","sequence":"additional","affiliation":[{"name":"From the Departments of Genetics, Hematology, and Pathology & Laboratory Medicine, University Medical Center Groningen, The Netherlands; and Cross Cancer Institute, Edmonton, AB, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bauke","family":"de Jong","sequence":"additional","affiliation":[{"name":"From the Departments of Genetics, Hematology, and Pathology & Laboratory Medicine, University Medical Center Groningen, The Netherlands; and Cross Cancer Institute, Edmonton, AB, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanessa","family":"Krause","sequence":"additional","affiliation":[{"name":"From the Departments of Genetics, Hematology, and Pathology & Laboratory Medicine, University Medical Center Groningen, The Netherlands; and Cross Cancer Institute, Edmonton, AB, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin C.","family":"Palmer","sequence":"additional","affiliation":[{"name":"From the Departments of Genetics, Hematology, and Pathology & Laboratory Medicine, University Medical Center Groningen, The Netherlands; and Cross Cancer Institute, Edmonton, AB, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Coupland","sequence":"additional","affiliation":[{"name":"From the Departments of Genetics, Hematology, and Pathology & Laboratory Medicine, University Medical Center Groningen, The Netherlands; and Cross Cancer Institute, Edmonton, AB, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip M.","family":"Kluin","sequence":"additional","affiliation":[{"name":"From the Departments of Genetics, Hematology, and Pathology & Laboratory Medicine, University Medical Center Groningen, The Netherlands; and Cross Cancer Institute, Edmonton, AB, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"van den Berg","sequence":"additional","affiliation":[{"name":"From the Departments of Genetics, Hematology, and Pathology & Laboratory Medicine, University Medical Center Groningen, The Netherlands; and Cross Cancer Institute, Edmonton, AB, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sibrand","family":"Poppema","sequence":"additional","affiliation":[{"name":"From the Departments of Genetics, Hematology, and Pathology & Laboratory Medicine, University Medical Center Groningen, The Netherlands; and Cross Cancer Institute, Edmonton, AB, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"234","reference":[{"key":"2019111610342155800_B1","doi-asserted-by":"crossref","first-page":"1361","DOI":"10.1182\/blood.V84.5.1361.1361","article-title":"A revised European-American classification of lymphoid neoplasms: a proposal from the International Lymphoma Study Group.","volume":"84","author":"Harris","year":"1994","journal-title":"Blood."},{"key":"2019111610342155800_B2","doi-asserted-by":"crossref","first-page":"3835","DOI":"10.1200\/JCO.1999.17.12.3835","article-title":"World Health Organization classification of neoplastic diseases of the hematopoietic and lymphoid tissues: report of the Clinical Advisory Committee meeting\u2014Airlie House, Virginia, November 1997.","volume":"17","author":"Harris","year":"1999","journal-title":"J Clin Oncol."},{"key":"2019111610342155800_B3","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1016\/S0140-6736(88)90367-4","article-title":"Updated Kiel classification for lymphomas.","volume":"1","author":"Stansfeld","year":"1988","journal-title":"Lancet."},{"key":"2019111610342155800_B4","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1002\/hon.2900010209","article-title":"Criteria for cytologic subclassification of follicular lymphomas: a proposed alternative method.","volume":"1","author":"Mann","year":"1982","journal-title":"Hematol Oncol."},{"key":"2019111610342155800_B5","doi-asserted-by":"crossref","first-page":"1349","DOI":"10.1200\/JCO.1994.12.7.1349","article-title":"Follicular large-cell lymphoma: intermediate or low grade?","volume":"12","author":"Bartlett","year":"1994","journal-title":"J Clin Oncol."},{"key":"2019111610342155800_B6","doi-asserted-by":"crossref","first-page":"1654","DOI":"10.1200\/JCO.1997.15.4.1654","article-title":"Follicular large-cell lymphoma treated with intensive chemotherapy: an analysis of 89 cases included in the LNH87 trial and comparison with the outcome of diffuse large B-cell lymphoma. 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