{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T13:02:19Z","timestamp":1791291739537,"version":"4.1.0"},"reference-count":46,"publisher":"American Society of Hematology","issue":"1","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2001,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In studies aimed at further characterizing the cellular immunodeficiency of the Wiskott-Aldrich syndrome (WAS), we found that T lymphocytes from WAS patients display abnormal chemotaxis in response to the T-cell chemoattractant stromal cell\u2013derived factor (SDF)-1. The Wiskott- Aldrich syndrome protein (WASP), together with the Rho family GTPase Cdc42, control stimulus-induced actin cytoskeleton rearrangements that are involved in cell motility. Because WASP is an effector of Cdc42, we further studied how Cdc42 and WASP are involved in SDF-1\u2013induced chemotaxis of T lymphocytes. We provide here direct evidence that SDF-1 activates Cdc42. We then specifically investigated the role of the interaction between Cdc42 and WASP in SDF-1\u2013responsive cells. This was achieved by abrogating this interaction with a recombinant polypeptide (TAT-CRIB), comprising the Cdc42\/Rac interactive binding (CRIB) domain of WASP and a human immunodeficiency virus\u2013TAT peptide that renders the fusion protein cell-permeant. This TAT-CRIB protein was shown to bind specifically to Cdc42-GTP and to inhibit the chemotactic response of a T-cell line to SDF-1. Altogether, these data demonstrate that Cdc42-WASP interaction is critical for SDF-1\u2013induced chemotaxis of T cells.<\/jats:p>","DOI":"10.1182\/blood.v97.1.33","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T12:46:47Z","timestamp":1027774007000},"page":"33-38","update-policy":"https:\/\/doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":165,"title":["The interaction between Cdc42 and WASP is required for SDF-1\u2013induced T-lymphocyte chemotaxis"],"prefix":"10.1182","volume":"97","author":[{"given":"Elie","family":"Haddad","sequence":"first","affiliation":[{"name":"From INSERM U362, Institut Gustave Roussy, Villejuif Cedex, France; INSERM U461, Faculte\u0301 de Pharmacie-Paris Sud, Chatenay-Malabry, France; Transfusion Medicine, University of Ulm, Ulm, Germany; and INSERM U429, Ho\u0302pital Necker Enfants Malades, Paris Cedex 15, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose\u0301 L.","family":"Zugaza","sequence":"additional","affiliation":[{"name":"From INSERM U362, Institut Gustave Roussy, Villejuif Cedex, France; INSERM U461, Faculte\u0301 de Pharmacie-Paris Sud, Chatenay-Malabry, France; Transfusion Medicine, University of Ulm, Ulm, Germany; and INSERM U429, Ho\u0302pital Necker Enfants Malades, Paris Cedex 15, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fawzia","family":"Louache","sequence":"additional","affiliation":[{"name":"From INSERM U362, Institut Gustave Roussy, Villejuif Cedex, France; INSERM U461, Faculte\u0301 de Pharmacie-Paris Sud, Chatenay-Malabry, France; Transfusion Medicine, University of Ulm, Ulm, Germany; and INSERM U429, Ho\u0302pital Necker Enfants Malades, Paris Cedex 15, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Najet","family":"Debili","sequence":"additional","affiliation":[{"name":"From INSERM U362, Institut Gustave Roussy, Villejuif Cedex, France; INSERM U461, Faculte\u0301 de Pharmacie-Paris Sud, Chatenay-Malabry, France; Transfusion Medicine, University of Ulm, Ulm, Germany; and INSERM U429, Ho\u0302pital Necker Enfants Malades, Paris Cedex 15, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Crouin","sequence":"additional","affiliation":[{"name":"From INSERM U362, Institut Gustave Roussy, Villejuif Cedex, France; INSERM U461, Faculte\u0301 de Pharmacie-Paris Sud, Chatenay-Malabry, France; Transfusion Medicine, University of Ulm, Ulm, Germany; and INSERM U429, Ho\u0302pital Necker Enfants Malades, Paris Cedex 15, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaus","family":"Schwarz","sequence":"additional","affiliation":[{"name":"From INSERM U362, Institut Gustave Roussy, Villejuif Cedex, France; INSERM U461, Faculte\u0301 de Pharmacie-Paris Sud, Chatenay-Malabry, France; Transfusion Medicine, University of Ulm, Ulm, Germany; and INSERM U429, Ho\u0302pital Necker Enfants Malades, Paris Cedex 15, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alain","family":"Fischer","sequence":"additional","affiliation":[{"name":"From INSERM U362, Institut Gustave Roussy, Villejuif Cedex, France; INSERM U461, Faculte\u0301 de Pharmacie-Paris Sud, Chatenay-Malabry, France; Transfusion Medicine, University of Ulm, Ulm, Germany; and INSERM U429, Ho\u0302pital Necker Enfants Malades, Paris Cedex 15, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William","family":"Vainchenker","sequence":"additional","affiliation":[{"name":"From INSERM U362, Institut Gustave Roussy, Villejuif Cedex, France; INSERM U461, Faculte\u0301 de Pharmacie-Paris Sud, Chatenay-Malabry, France; Transfusion Medicine, University of Ulm, Ulm, Germany; and INSERM U429, Ho\u0302pital Necker Enfants Malades, Paris Cedex 15, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacques","family":"Bertoglio","sequence":"additional","affiliation":[{"name":"From INSERM U362, Institut Gustave Roussy, Villejuif Cedex, France; INSERM U461, Faculte\u0301 de Pharmacie-Paris Sud, Chatenay-Malabry, France; Transfusion Medicine, University of Ulm, Ulm, Germany; and INSERM U429, Ho\u0302pital Necker Enfants Malades, Paris Cedex 15, France."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"234","reference":[{"key":"2020021216201584300_B1","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1007\/BF00792601","article-title":"The Wiskott-Aldrich syndrome.","volume":"19","author":"Ochs","year":"1998","journal-title":"Springer Semin Immunopathol."},{"key":"2020021216201584300_B2","doi-asserted-by":"crossref","first-page":"5326","DOI":"10.1002\/j.1460-2075.1996.tb00917.x","article-title":"N-WASP, a novel actin-depolymerizing protein, regulates the cortical cytoskeletal rearrangement 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