{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T02:19:30Z","timestamp":1787019570451,"version":"build-2736575974"},"reference-count":36,"publisher":"American Society of Hematology","issue":"10","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2001,5,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Childhood T-cell acute lymphoblastic leukemia (T-ALL) is one of the most common childhood cancers. It is reported that preconditioning sublethally irradiated immunodeficient NOD\/SCID (nonobese diabetic\/X-linked severe combined immunodeficient) mice with human cord blood mononuclear cells facilitates the engraftment, expansion, and dissemination in these mice of primary T-ALL cells obtained from patients at the time of diagnosis. Cells recovered from mouse bone marrow or spleen resembled the original leukemia cells from patients with respect to surface lineage markers and T-cell receptor V\u03b2 gene rearrangements. Moreover, the pattern of leukemia dissemination in mouse tissues, resulting in universally fatal leukemia, is reminiscent of the human clinical disease. In addition, the fidelity of the model to the human disease is documented with regard to the presence of morphologically identifiable human leukemia cells in mouse bone marrow and blood and the maintenance of leukemia-initiating capacity within the leukemia-engrafted mouse. Therefore, several lines of independent approaches are used to suggest that the engrafted cells are of human leukemia origin and are not derived from cord blood. The in vivo model described here should enable the study of the growth properties of primary T-ALL cells obtained from patients and should prove useful in evaluating the potential efficacy of therapeutic strategies directed toward T-ALL.<\/jats:p>","DOI":"10.1182\/blood.v97.10.3218","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T08:46:40Z","timestamp":1027759600000},"page":"3218-3225","update-policy":"https:\/\/doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":16,"title":["Preconditioning with fetal cord blood facilitates engraftment of primary childhood T-cell acute lymphoblastic leukemia in immunodeficient mice"],"prefix":"10.1182","volume":"97","author":[{"given":"Deno P.","family":"Dialynas","sequence":"first","affiliation":[{"name":"From the Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla; the Department of Pediatrics, University of California at San Diego Medical Center; the Immunology Program, Sidney Kimmel Cancer Center, San Diego, CA; and the Department of Pathology, Johns Hopkins University, Baltimore, MD."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mi-Jeong","family":"Lee","sequence":"additional","affiliation":[{"name":"From the Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla; the Department of Pediatrics, University of California at San Diego Medical Center; the Immunology Program, Sidney Kimmel Cancer Center, San Diego, CA; and the Department of Pathology, Johns Hopkins University, Baltimore, MD."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel P.","family":"Gold","sequence":"additional","affiliation":[{"name":"From the Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla; the Department of Pediatrics, University of California at San Diego Medical Center; the Immunology Program, Sidney Kimmel Cancer Center, San Diego, CA; and the Department of Pathology, Johns Hopkins University, Baltimore, MD."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-en","family":"Shao","sequence":"additional","affiliation":[{"name":"From the Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla; the Department of Pediatrics, University of California at San Diego Medical Center; the Immunology Program, Sidney Kimmel Cancer Center, San Diego, CA; and the Department of Pathology, Johns Hopkins University, Baltimore, MD."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alice L.","family":"Yu","sequence":"additional","affiliation":[{"name":"From the Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla; the Department of Pediatrics, University of California at San Diego Medical Center; the Immunology Program, Sidney Kimmel Cancer Center, San Diego, CA; and the Department of Pathology, Johns Hopkins University, Baltimore, MD."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J.","family":"Borowitz","sequence":"additional","affiliation":[{"name":"From the Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla; the Department of Pediatrics, University of California at San Diego Medical Center; the Immunology Program, Sidney Kimmel Cancer Center, San Diego, CA; and the Department of Pathology, Johns Hopkins University, Baltimore, MD."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Yu","sequence":"additional","affiliation":[{"name":"From the Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla; the Department of Pediatrics, University of California at San Diego Medical Center; the Immunology Program, Sidney Kimmel Cancer Center, San Diego, CA; and the Department of Pathology, Johns Hopkins University, Baltimore, MD."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"234","reference":[{"key":"2020021221573935600_B1","doi-asserted-by":"crossref","first-page":"4243","DOI":"10.1182\/blood.V90.11.4243","article-title":"Fifty years of studies of the biology and therapy of childhood leukemia.","volume":"90","author":"Kersey","year":"1997","journal-title":"Blood."},{"key":"2020021221573935600_B2","first-page":"10","article-title":"Establishment of continuous cultures of T-cell acute lymphoblastic leukemia cells at diagnosis.","volume":"50","author":"Gjerset","year":"1990","journal-title":"Cancer Res."},{"key":"2020021221573935600_B3","doi-asserted-by":"crossref","first-page":"712","DOI":"10.1182\/blood.V52.4.712.712","article-title":"Colony formation in vitro by leukemic cells in acute lymphoblastic leukemia (ALL).","volume":"52","author":"Smith","year":"1978","journal-title":"Blood."},{"key":"2020021221573935600_B4","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1182\/blood.V70.1.192.192","article-title":"Growth factor requirements of childhood acute leukemia: establishment of GM-CSF\u2013dependent cell lines.","volume":"70","author":"Lange","year":"1987","journal-title":"Blood."},{"key":"2020021221573935600_B5","first-page":"5657","article-title":"Monoclonal antibody and enzymatic prolifes of human malignant T-lymphoid cells and derived cell lines.","volume":"44","author":"Smith","year":"1984","journal-title":"Cancer Res."},{"key":"2020021221573935600_B6","doi-asserted-by":"crossref","first-page":"1597","DOI":"10.1126\/science.2595371","article-title":"A model of human acute lymphoblastic leukemia in immune-deficient SCID mice.","volume":"246","author":"Kamel-Reid","year":"1989","journal-title":"Science."},{"key":"2020021221573935600_B7","doi-asserted-by":"crossref","first-page":"2463","DOI":"10.1182\/blood.V77.11.2463.2463","article-title":"Homing and progression patterns of childhood actue lymphoblastic leukemias in severe combined immunodeficiency mice.","volume":"77","author":"Cesano","year":"1991","journal-title":"Blood."},{"key":"2020021221573935600_B8","doi-asserted-by":"crossref","first-page":"2015","DOI":"10.1182\/blood.V90.5.2015","article-title":"Growth of human T-cell lineage acute leukemia in severe combined immunodeficiency (SCID) mice and non-obese diabetic SCID mice.","volume":"90","author":"Steele","year":"1997","journal-title":"Blood."},{"key":"2020021221573935600_B9","doi-asserted-by":"crossref","first-page":"2489","DOI":"10.1182\/blood.V83.9.2489.bloodjournal8392489","article-title":"Immature human cord blood progenitors engraft and proliferate to high levels in severe combined immunodeficient mice.","volume":"83","author":"Vormoor","year":"1994","journal-title":"Blood."},{"key":"2020021221573935600_B10","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1182\/blood.V90.1.85","article-title":"Engraftment and development of human CD34+ -enriched cells from umbilical cord blood in NOD\/LtSz-SCID\/SCID mice.","volume":"90","author":"Hogan","year":"1997","journal-title":"Blood."},{"key":"2020021221573935600_B11","doi-asserted-by":"crossref","first-page":"180","DOI":"10.4049\/jimmunol.154.1.180","article-title":"Multiple defects in innate and adaptive immunologic function in NOD\/LtsZ-SCID mice.","volume":"154","author":"Shultz","year":"1995","journal-title":"J Immunol."},{"key":"2020021221573935600_B12","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/S0165-5728(97)00028-3","article-title":"Results of a phase I clinical trial of a T cell receptor peptide vaccine in patients with multiple sclerosis, I: analysis of T cell receptor utilization in CSF cell population.","volume":"76","author":"Wilson","year":"1997","journal-title":"J Neuroimmunol."},{"key":"2020021221573935600_B13","first-page":"139","article-title":"The study of human neoplastic disease in severe combined immunodeficient mice.","volume":"43","author":"Williams","year":"1993","journal-title":"Lab Anim Sci."},{"key":"2020021221573935600_B14","doi-asserted-by":"crossref","first-page":"1135","DOI":"10.1182\/blood.V88.4.1135.bloodjournal8841135","article-title":"Severe combined immunodeficient mouse models of human leukemia.","volume":"88","author":"Uckun","year":"1996","journal-title":"Blood."},{"key":"2020021221573935600_B15","first-page":"767","article-title":"Human leukemia colony enhancing activity derived from bone marrow of the human cord blood reconstituted NOD\/SCID mice [abstract].","volume":"96","author":"Shao","year":"2000","journal-title":"Blood."},{"key":"2020021221573935600_B16","first-page":"8032","article-title":"Reconstitution of SCID mice with human lymphoid and myeloid cells after transplantation with human fetal bone marrow without the requirement for exogenous human cytokines.","volume":"91","author":"Kollmann","year":"1994","journal-title":"Immunology."},{"key":"2020021221573935600_B17","first-page":"281","article-title":"Regulation and reconstitution of human hematopoiesis.","volume":"95","author":"Yu","year":"1996","journal-title":"J Formos Med Assoc."},{"key":"2020021221573935600_B18","doi-asserted-by":"crossref","first-page":"3731","DOI":"10.1182\/blood.V88.10.3731.bloodjournal88103731","article-title":"Phenotype and function of human hematopoietic cells engrafting immune-deficient CB17-severe combined immunodeficiency mice and nonobese diabetic-severe combined immunodeficiency mice after transplantation of human cord blood mononuclear cells.","volume":"88","author":"Pflumio","year":"1996","journal-title":"Blood."},{"key":"2020021221573935600_B19","doi-asserted-by":"crossref","first-page":"958","DOI":"10.1126\/science.286.5441.958","article-title":"A direct estimate of the human \u03b1\u03b2 T cell receptor diversity.","volume":"286","author":"Arstila","year":"1999","journal-title":"Science."},{"key":"2020021221573935600_B20","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1002\/eji.1830240410","article-title":"Bimodal distribution of V\u03b22+CD4+ T cells in human peripheral blood.","volume":"24","author":"Clarke","year":"1994","journal-title":"Eur J Immunol."},{"key":"2020021221573935600_B21","doi-asserted-by":"crossref","first-page":"1391","DOI":"10.1084\/jem.181.4.1391","article-title":"Dual T cell receptor \u03b2 chain expression on human T lymphocytes.","volume":"181","author":"Davodeau","year":"1995","journal-title":"J Exp Med."},{"key":"2020021221573935600_B22","doi-asserted-by":"crossref","first-page":"1587","DOI":"10.1084\/jem.181.4.1587","article-title":"Normal T lymphocytes can express two different T cell receptor \u03b2 chains: implications for the mechanism of allelic exclusion.","volume":"181","author":"Padovan","year":"1995","journal-title":"J Exp Med."},{"key":"2020021221573935600_B23","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1002\/stem.170242","article-title":"Human hematopoietic stem\/progenitor cells generate CD5+ B lymphoid cells in NOD\/SCID mice.","volume":"17","author":"Novelli","year":"2000","journal-title":"Stem Cells."},{"key":"2020021221573935600_B24","first-page":"17","article-title":"In vitro and in vivo assessment of the graft-versus-leukemia activity of cord blood.","volume":"15","author":"Harris","year":"1995","journal-title":"Bone Marrow Transplant."},{"key":"2020021221573935600_B25","doi-asserted-by":"crossref","first-page":"1761","DOI":"10.1182\/blood.V94.5.1761","article-title":"Growth characteristics of acute myelogenous leukemia progenitors that initiate malignant hematopoiesis in nonobese diabetic\/severe combined immunodeficient mice.","volume":"94","author":"Ailles","year":"1999","journal-title":"Blood."},{"key":"2020021221573935600_B26","first-page":"2103","article-title":"Use of colony assays and anti-T cell immunotoxins to elucidate the immunobiologic features of leukemic progenitor cells in T-lineage acute lymphoblastic leukemia.","volume":"140","author":"Uckun","year":"1988","journal-title":"Immunology."},{"key":"2020021221573935600_B27","doi-asserted-by":"crossref","first-page":"1723","DOI":"10.1182\/blood.V76.9.1723.1723","article-title":"Autologous bone marrow transplantation in high-risk remission T-lineage acute lymphoblastic leukemia using immunotoxins plus 4-hydroperoxycyclophosphamide for marrow purging.","volume":"76","author":"Uckun","year":"1990","journal-title":"Blood."},{"key":"2020021221573935600_B28","doi-asserted-by":"crossref","first-page":"1088","DOI":"10.1182\/blood.V68.5.1088.1088","article-title":"Acute lymphoblastic leukemia and non-Hodgkin's lymphoma of T lineage: colony-forming cells retain growth factor (interleukin 2) dependence.","volume":"68","author":"Touw","year":"1986","journal-title":"Blood."},{"key":"2020021221573935600_B29","doi-asserted-by":"crossref","first-page":"556","DOI":"10.1182\/blood.V66.3.556.556","article-title":"Common and pre-B acute lymphoblastic leukemia cells express interleukin 2 receptors, and interleukin 2 stimulates in vitro colony formation.","volume":"66","author":"Touw","year":"1985","journal-title":"Blood."},{"key":"2020021221573935600_B30","doi-asserted-by":"crossref","first-page":"2056","DOI":"10.1182\/blood.V94.6.2056","article-title":"Isolation of a highly quiescent subpopulation of primitive leukemic cells in chronic myeloid leukemia.","volume":"94","author":"Holyoake","year":"1999","journal-title":"Blood."},{"key":"2020021221573935600_B31","doi-asserted-by":"crossref","first-page":"730","DOI":"10.1038\/nm0797-730","article-title":"Human acute myeloid leukemia is organized as a hierarchy that originates from a primitive hematopoietic cell.","volume":"3","author":"Bonnet","year":"1997","journal-title":"Nat Med."},{"key":"2020021221573935600_B32","doi-asserted-by":"crossref","first-page":"2187","DOI":"10.1182\/blood.V87.6.2187.bloodjournal8762187","article-title":"Long-term leukemia-initiating capacity of a CD34\u2212 subpopulation of acute myeloid leukemia.","volume":"87","author":"Terpstra","year":"1996","journal-title":"Blood."},{"key":"2020021221573935600_B33","doi-asserted-by":"crossref","first-page":"2436","DOI":"10.1182\/blood.V84.8.2436.2436","article-title":"Phenotypic characterization of a novel bone marrow derived cell that facilitates engraftment of allogeneic bone marrow stem cells.","volume":"84","author":"Kaufman","year":"1994","journal-title":"Blood."},{"key":"2020021221573935600_B34","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1126\/science.283.5403.845","article-title":"Dependence of human stem cell engraftment and repopulation of NOD\/SCID mice on CXCR4.","volume":"283","author":"Peled","year":"1999","journal-title":"Science."},{"key":"2020021221573935600_B35","first-page":"660","article-title":"Fetal cord blood facilitates engraftment of primary childhood T-cell acute lymphoblastic leukemia in immunodeficient mice and enhances leukemia colony formation in vitro [abstract].","volume":"40","author":"Yu","year":"1999","journal-title":"Proc Am Assoc Cancer Res."},{"key":"2020021221573935600_B36","doi-asserted-by":"crossref","first-page":"1041","DOI":"10.1016\/S0145-2126(00)00076-X","article-title":"Human cord blood conditioned medium enhances leukemia colony formation in vitro.","volume":"24","author":"Shao","year":"2000","journal-title":"Leuk Res."}],"container-title":["Blood"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/ashpublications.org\/blood\/article-pdf\/97\/10\/3218\/1673423\/h8100103218.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/ashpublications.org\/blood\/article-pdf\/97\/10\/3218\/1673423\/h8100103218.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,23]],"date-time":"2023-04-23T16:10:48Z","timestamp":1682266248000},"score":1,"resource":{"primary":{"URL":"https:\/\/ashpublications.org\/blood\/article\/97\/10\/3218\/89666\/Preconditioning-with-fetal-cord-blood-facilitates"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,5,15]]},"references-count":36,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2001,5,15]]}},"URL":"https:\/\/doi.org\/10.1182\/blood.v97.10.3218","relation":{},"ISSN":["1528-0020","0006-4971"],"issn-type":[{"value":"1528-0020","type":"electronic"},{"value":"0006-4971","type":"print"}],"subject":[],"published":{"date-parts":[[2001,5,15]]}}}