{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T07:53:14Z","timestamp":1770969194622,"version":"3.50.1"},"reference-count":36,"publisher":"American Society of Hematology","issue":"3","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2001,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>TEL-AML1 fusion resulting from the t(12;21)(p13;q22) is one of the most common genetic abnormalities in childhood acute lymphoblastic leukemia. Recent findings that site-specific cleavage of the MLL gene can be induced by chemotherapeutic agents such as topoisomerase-II inhibitors suggest that apoptogenic agents can cause chromosomal translocations in hematopoietic cells. This study demonstrates a possible relationship between exposure to apoptogenic stimuli, TEL breaks, and the formation ofTEL-AML1 fusion in immature B lymphocytes. Short-term culture of immature B cell lines in the presence of apoptogenic stimuli such as serum starvation, etoposide, or salicylic acid induced double-strand breaks (DSBs) in intron 5 of the TEL gene and intron 1 of the AML1 gene. TEL-AML1fusion transcripts were also identified by reverse transcriptase\u2013polymerase chain reaction (RT-PCR) analysis in cell lines treated by serum starvation or aminophylline. DSBs within theTEL gene were also associated with fusion to other unknown genes, presumably as a result of chromosomal translocation. We also examined 67 cord blood and 147 normal peripheral blood samples for the existence of in-frame TEL-AML1 fusion transcripts. One cord blood sample (1.5%) and 13 normal peripheral blood samples (8.8%) were positive as detected by nested RT-PCR. These data suggest that breakage and fusion of TEL andAML1 may be relatively common events and that sublethal apoptotic signals could play a role in initiating leukemogenesis via the promotion of DNA damage.<\/jats:p>","DOI":"10.1182\/blood.v97.3.737","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T08:46:47Z","timestamp":1027759607000},"page":"737-743","update-policy":"https:\/\/doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":64,"title":["Breakage and fusion of the TEL (ETV6) gene in immature B lymphocytes induced by apoptogenic signals"],"prefix":"10.1182","volume":"97","author":[{"given":"Minenori","family":"Eguchi-Ishimae","sequence":"first","affiliation":[{"name":"From the Department of Virology, National Children's Medical Research Center, Tokyo; Department of Cancer Cytogenetics, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima; Division of Pediatrics, Hamanomachi Hospital, Fukuoka; Department of Pediatrics, Saga University School of Medicine, Saga; and Department of Pediatrics, Hiroshima University School of Medicine, Hiroshima, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariko","family":"Eguchi","sequence":"additional","affiliation":[{"name":"From the Department of Virology, National Children's Medical Research Center, Tokyo; Department of Cancer Cytogenetics, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima; Division of Pediatrics, Hamanomachi Hospital, Fukuoka; Department of Pediatrics, Saga University School of Medicine, Saga; and Department of Pediatrics, Hiroshima University School of Medicine, Hiroshima, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eiichi","family":"Ishii","sequence":"additional","affiliation":[{"name":"From the Department of Virology, National Children's Medical Research Center, Tokyo; Department of Cancer Cytogenetics, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima; Division of Pediatrics, Hamanomachi Hospital, Fukuoka; Department of Pediatrics, Saga University School of Medicine, Saga; and Department of Pediatrics, Hiroshima University School of Medicine, Hiroshima, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sumio","family":"Miyazaki","sequence":"additional","affiliation":[{"name":"From the Department of Virology, National Children's Medical Research Center, Tokyo; Department of Cancer Cytogenetics, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima; Division of Pediatrics, Hamanomachi Hospital, Fukuoka; Department of Pediatrics, Saga University School of Medicine, Saga; and Department of Pediatrics, Hiroshima University School of Medicine, Hiroshima, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuhiro","family":"Ueda","sequence":"additional","affiliation":[{"name":"From the Department of Virology, National Children's Medical Research Center, Tokyo; Department of Cancer Cytogenetics, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima; Division of Pediatrics, Hamanomachi Hospital, Fukuoka; Department of Pediatrics, Saga University School of Medicine, Saga; and Department of Pediatrics, Hiroshima University School of Medicine, Hiroshima, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nanao","family":"Kamada","sequence":"additional","affiliation":[{"name":"From the Department of Virology, National Children's Medical Research Center, Tokyo; Department of Cancer Cytogenetics, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima; Division of Pediatrics, Hamanomachi Hospital, Fukuoka; Department of Pediatrics, Saga University School of Medicine, Saga; and Department of Pediatrics, Hiroshima University School of Medicine, Hiroshima, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuki","family":"Mizutani","sequence":"additional","affiliation":[{"name":"From the Department of Virology, National Children's Medical Research Center, Tokyo; Department of Cancer Cytogenetics, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima; Division of Pediatrics, Hamanomachi Hospital, Fukuoka; Department of Pediatrics, Saga University School of Medicine, Saga; and Department of Pediatrics, Hiroshima University School of Medicine, Hiroshima, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"234","reference":[{"key":"2020021221153719300_B1","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1002\/gcc.2870090307","article-title":"t(12;21): a new recurrent translocation in acute lymphoblastic 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