{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T21:06:22Z","timestamp":1760907982090},"reference-count":29,"publisher":"American Society of Hematology","issue":"4","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2001,2,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Substantial numbers of human mast cells (MCs) were generated from umbilical cord blood (CB) and from adult peripheral blood (PB). A single CB progenitor produced 15\u2009436 MCs, whereas a single PB progenitor produced 807 MCs on average. However, PB-derived MCs were far more active than CB-derived MCs in terms of high-affinity IgE receptor (Fc\u03b5RI)-mediated reactions. One million sensitized PB-derived MCs released 3.6 \u03bcg histamine, 215 pg IL-5, and 14 ng granulocyte macrophage\u2013colony-stimulating factor (GM-CSF), whereas 106 sensitized CB-derived MCs released only 0.8 \u03bcg histamine, 31 pg IL-5, and 0.58 ng GM-CSF on anti-IgE challenge. However, ionophore A23\u2009187 released similar levels of histamine from the 2 MC types. PB-derived MCs highly expressed surface Fc\u03b5RI \u03b1 chain, and CB-derived MCs almost lacked it in the absence of IgE. PB-derived MCs expressed approximately 5 times higher levels of messenger RNA (mRNA) for Fc\u03b5RI \u03b1 chain than CB-derived MCs, but mRNAs for \u03b2 and \u03b3 chains of the receptors were equally expressed. Among the approximately 5600 kinds of full-length human genes examined by using the high-density oligonucleotide probe-array system, Fc\u03b5RI\u03b1 was ranked the fifth most increased transcript in PB-derived MCs. The 4 other increased transcripts were unrelated to MC function. These results suggest that IgE-mediated reactions may be restricted during early infancy through the selective inhibition of Fc\u03b5RI\u03b1 transcription, which is probably committed at progenitor stages and is, at least in part, cytokine-insensitive.<\/jats:p>","DOI":"10.1182\/blood.v97.4.1016","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T12:46:47Z","timestamp":1027774007000},"page":"1016-1022","update-policy":"http:\/\/dx.doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":50,"title":["Selective down-regulation of high-affinity IgE receptor (Fc\u03b5RI) \u03b1-chain messenger RNA among transcriptome in cord blood\u2013derived versus adult peripheral blood\u2013derived cultured human mast cells"],"prefix":"10.1182","volume":"97","author":[{"given":"Makoto","family":"Iida","sequence":"first","affiliation":[{"name":"From the Departments of Allergy and Immunology, National Children's Medical Research Center, Tokyo; Genox Research, Kanagawa; Department of Medicine, Gunma University School of Medicine, Gunma; Molecular and Cellular Biology Group, Medical Research Laboratories, and the Ethical Business Strategy Division, Taisho Pharmaceutical, Saitama; and Allergy Research Center, Juntendo University School of Medicine, Tokyo, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenji","family":"Matsumoto","sequence":"additional","affiliation":[{"name":"From the Departments of Allergy and Immunology, National Children's Medical Research Center, Tokyo; Genox Research, Kanagawa; Department of Medicine, Gunma University School of Medicine, Gunma; Molecular and Cellular Biology Group, Medical Research Laboratories, and the Ethical Business Strategy Division, Taisho Pharmaceutical, Saitama; and Allergy Research Center, Juntendo University School of Medicine, Tokyo, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hisashi","family":"Tomita","sequence":"additional","affiliation":[{"name":"From the Departments of Allergy and Immunology, National Children's Medical Research Center, Tokyo; Genox Research, Kanagawa; Department of Medicine, Gunma University School of Medicine, Gunma; Molecular and Cellular Biology Group, Medical Research Laboratories, and the Ethical Business Strategy Division, Taisho Pharmaceutical, Saitama; and Allergy Research Center, Juntendo University School of Medicine, Tokyo, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshiharu","family":"Nakajima","sequence":"additional","affiliation":[{"name":"From the Departments of Allergy and Immunology, National Children's Medical Research Center, Tokyo; Genox Research, Kanagawa; Department of Medicine, Gunma University School of Medicine, Gunma; Molecular and Cellular Biology Group, Medical Research Laboratories, and the Ethical Business Strategy Division, Taisho Pharmaceutical, Saitama; and Allergy Research Center, Juntendo University School of Medicine, Tokyo, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akira","family":"Akasawa","sequence":"additional","affiliation":[{"name":"From the Departments of Allergy and Immunology, National Children's Medical Research Center, Tokyo; Genox Research, Kanagawa; Department of Medicine, Gunma University School of Medicine, Gunma; Molecular and Cellular Biology Group, Medical Research Laboratories, and the Ethical Business Strategy Division, Taisho Pharmaceutical, Saitama; and Allergy Research Center, Juntendo University School of Medicine, Tokyo, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noriko Yuyama","family":"Ohtani","sequence":"additional","affiliation":[{"name":"From the Departments of Allergy and Immunology, National Children's Medical Research Center, Tokyo; Genox Research, Kanagawa; Department of Medicine, Gunma University School of Medicine, Gunma; Molecular and Cellular Biology Group, Medical Research Laboratories, and the Ethical Business Strategy Division, Taisho Pharmaceutical, Saitama; and Allergy Research Center, Juntendo University School of Medicine, Tokyo, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ning Lu","family":"Yoshida","sequence":"additional","affiliation":[{"name":"From the Departments of Allergy and Immunology, National Children's Medical Research Center, Tokyo; Genox Research, Kanagawa; Department of Medicine, Gunma University School of Medicine, Gunma; Molecular and Cellular Biology Group, Medical Research Laboratories, and the Ethical Business Strategy Division, Taisho Pharmaceutical, Saitama; and Allergy Research Center, Juntendo University School of Medicine, Tokyo, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keiko","family":"Matsui","sequence":"additional","affiliation":[{"name":"From the Departments of Allergy and Immunology, National Children's Medical Research Center, Tokyo; Genox Research, Kanagawa; Department of Medicine, Gunma University School of Medicine, Gunma; Molecular and Cellular Biology Group, Medical Research Laboratories, and the Ethical Business Strategy Division, Taisho Pharmaceutical, Saitama; and Allergy Research Center, Juntendo University School of Medicine, Tokyo, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akiko","family":"Nakada","sequence":"additional","affiliation":[{"name":"From the Departments of Allergy and Immunology, National Children's Medical Research Center, Tokyo; Genox Research, Kanagawa; Department of Medicine, Gunma University School of Medicine, Gunma; Molecular and Cellular Biology Group, Medical Research Laboratories, and the Ethical Business Strategy Division, Taisho Pharmaceutical, Saitama; and Allergy Research Center, Juntendo University School of Medicine, Tokyo, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuji","family":"Sugita","sequence":"additional","affiliation":[{"name":"From the Departments of Allergy and Immunology, National Children's Medical Research Center, Tokyo; Genox Research, Kanagawa; Department of Medicine, Gunma University School of Medicine, Gunma; Molecular and Cellular Biology Group, Medical Research Laboratories, and the Ethical Business Strategy Division, Taisho Pharmaceutical, Saitama; and Allergy Research Center, Juntendo University School of Medicine, Tokyo, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuji","family":"Shimizu","sequence":"additional","affiliation":[{"name":"From the Departments of Allergy and Immunology, National Children's Medical Research Center, Tokyo; Genox Research, Kanagawa; Department of Medicine, Gunma University School of Medicine, Gunma; Molecular and Cellular Biology Group, Medical Research Laboratories, and the Ethical Business Strategy Division, Taisho Pharmaceutical, Saitama; and Allergy Research Center, Juntendo University School of Medicine, Tokyo, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shunichi","family":"Wakahara","sequence":"additional","affiliation":[{"name":"From the Departments of Allergy and Immunology, National Children's Medical Research Center, Tokyo; Genox Research, Kanagawa; Department of Medicine, Gunma University School of Medicine, Gunma; Molecular and Cellular Biology Group, Medical Research Laboratories, and the Ethical Business Strategy Division, Taisho Pharmaceutical, Saitama; and Allergy Research Center, Juntendo University School of Medicine, Tokyo, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toru","family":"Nakao","sequence":"additional","affiliation":[{"name":"From the Departments of Allergy and Immunology, National Children's Medical Research Center, Tokyo; Genox Research, Kanagawa; Department of Medicine, Gunma University School 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