{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T05:08:10Z","timestamp":1790485690411,"version":"4.1.0"},"reference-count":33,"publisher":"American Society of Hematology","issue":"4","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2001,2,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The snake venom C-type lectin alboaggregin A (or 50-kd alboaggregin) from Trimeresurus albolabris was previously shown to be a platelet glycoprotein (GP) Ib agonist. However, investigations of the signal transduction induced in platelets showed patterns of tyrosine phosphorylation that were different from those of other GPIb agonists and suggested the presence of an additional receptor. In this study, the binding of biotinylated alboaggregin A to platelet lysates, as well as affinity chromatography evaluations of platelet lysates on an alboaggregin A\u2013coated column, indicated that this other receptor is GPVI. Additional experiments with reagents that inhibit either GPIb or GPVI specifically supported this finding. These experiments also showed that both GPIb and GPVI have a role in the combined signaling and that the overall direction this takes can be influenced by inhibitors of one or the other receptor pathway.<\/jats:p>","DOI":"10.1182\/blood.v97.4.929","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T08:46:47Z","timestamp":1027759607000},"page":"929-936","update-policy":"https:\/\/doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":54,"title":["Alboaggregin A activates platelets by a mechanism involving glycoprotein VI as well as glycoprotein Ib"],"prefix":"10.1182","volume":"97","author":[{"given":"Dagmar","family":"Do\u0308rmann","sequence":"first","affiliation":[{"name":"From the Theodor Kocher Institute, University of Berne, Berne, Switzerland; and the Klinik und Poliklinik fu\u0308r Ana\u0308sthesiologie und Operative Intensivmedizin, Experimental and Clinical Haemostasis, University of Mu\u0308nster, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeannine M.","family":"Clemetson","sequence":"additional","affiliation":[{"name":"From the Theodor Kocher Institute, University of Berne, Berne, Switzerland; and the Klinik und Poliklinik fu\u0308r Ana\u0308sthesiologie und Operative Intensivmedizin, Experimental and Clinical Haemostasis, University of Mu\u0308nster, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexei","family":"Navdaev","sequence":"additional","affiliation":[{"name":"From the Theodor Kocher Institute, University of Berne, Berne, Switzerland; and the Klinik und Poliklinik fu\u0308r Ana\u0308sthesiologie und Operative Intensivmedizin, Experimental and Clinical Haemostasis, University of Mu\u0308nster, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beate E.","family":"Kehrel","sequence":"additional","affiliation":[{"name":"From the Theodor Kocher Institute, University of Berne, Berne, Switzerland; and the Klinik und Poliklinik fu\u0308r Ana\u0308sthesiologie und Operative Intensivmedizin, Experimental and Clinical Haemostasis, University of Mu\u0308nster, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth J.","family":"Clemetson","sequence":"additional","affiliation":[{"name":"From the Theodor Kocher Institute, University of Berne, Berne, Switzerland; and the Klinik und Poliklinik fu\u0308r Ana\u0308sthesiologie und Operative Intensivmedizin, Experimental and Clinical Haemostasis, University of Mu\u0308nster, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"234","reference":[{"key":"2020021217471406500_B1","doi-asserted-by":"crossref","first-page":"945","DOI":"10.1016\/0041-0101(92)90040-C","article-title":"Characterization of snake venom components acting on blood coagulation and platelet function.","volume":"30","author":"Ouyang","year":"1992","journal-title":"Toxicon."},{"key":"2020021217471406500_B2","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1016\/S0041-0101(99)00187-7","article-title":"Snake venom modulators of platelet adhesion receptors and their ligands.","volume":"38","author":"Andrews","year":"2000","journal-title":"Toxicon."},{"key":"2020021217471406500_B3","doi-asserted-by":"crossref","first-page":"730","DOI":"10.1038\/207730a0","article-title":"Actions of some coagulant snake venoms on blood platelets.","volume":"207","author":"Davey","year":"1965","journal-title":"Nature."},{"key":"2020021217471406500_B4","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/S0167-4838(99)00268-X","article-title":"Snake venom proteases affecting hemostasis and thrombosis.","volume":"1477","author":"Matsui","year":"2000","journal-title":"Biochim Biophys Acta."},{"key":"2020021217471406500_B5","doi-asserted-by":"crossref","first-page":"109","DOI":"10.3181\/00379727-219-44322","article-title":"Viper venom disintegrins and related molecules.","volume":"219","author":"McLane","year":"1998","journal-title":"Proc Soc Exp Biol Med."},{"key":"2020021217471406500_B6","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1007\/s000180050181","article-title":"What have snakes told us about integrins?","volume":"54","author":"Huang","year":"1998","journal-title":"Cell Mol Life Sci."},{"key":"2020021217471406500_B7","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/10408449991349168","article-title":"Snake venom hemorrhagins.","volume":"29","author":"Hati","year":"1999","journal-title":"Crit Rev Toxicol."},{"key":"2020021217471406500_B8","doi-asserted-by":"crossref","first-page":"928","DOI":"10.1073\/pnas.90.3.928","article-title":"Primary structure of two-chain botrocetin, a von Willebrand factor modulator purified from the venom of Bothrops jararaca.","volume":"90","author":"Usami","year":"1993","journal-title":"Proc Natl Acad Sci U S A."},{"key":"2020021217471406500_B9","doi-asserted-by":"crossref","first-page":"2321","DOI":"10.1182\/blood.V81.9.2321.2321","article-title":"Echicetin: a snake venom protein that inhibits binding of von Willebrand factor and alboaggregins to platelet glycoprotein Ib.","volume":"81","author":"Peng","year":"1993","journal-title":"Blood."},{"key":"2020021217471406500_B10","doi-asserted-by":"crossref","first-page":"10794","DOI":"10.1021\/bi00091a034","article-title":"Bothrojaracin, a new thrombin inhibitor isolated from Bothrops jararaca venom: characterization and mechanism of thrombin inhibition.","volume":"32","author":"Zingali","year":"1993","journal-title":"Biochemistry."},{"key":"2020021217471406500_B11","doi-asserted-by":"crossref","first-page":"947","DOI":"10.1042\/bj3080947","article-title":"Tokaracetin, a new platelet antagonist that binds to platelet glycoprotein Ib and inhibits von Willebrand factor-dependent shear-induced platelet aggregation.","volume":"308","author":"Kawasaki","year":"1995","journal-title":"Biochem J."},{"key":"2020021217471406500_B12","doi-asserted-by":"crossref","first-page":"10635","DOI":"10.1074\/jbc.271.18.10635","article-title":"Complete amino acid sequence and identification of the platelet glycoprotein Ib-binding site of jararaca GPIb-BP, a snake venom protein isolated from Bothrops jararaca.","volume":"271","author":"Kawasaki","year":"1996","journal-title":"J Biol Chem."},{"key":"2020021217471406500_B13","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1055\/s-0037-1613767","article-title":"Glycoprotein Ib-binding protein from the venom of Deinagkistrodon acutus\u2014cDNA sequence, functional characterization, and three-dimensional modeling.","volume":"83","author":"Chen","year":"2000","journal-title":"Thromb Haemost."},{"key":"2020021217471406500_B14","doi-asserted-by":"crossref","first-page":"12629","DOI":"10.1021\/bi960704e","article-title":"Binding of a novel 50-kilodalton alboaggregin from Trimeresurus albolabris and related viper venom proteins to the platelet membrane glycoprotein Ib-IX-V complex. 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