{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T04:17:52Z","timestamp":1791346672259,"version":"4.3.1"},"reference-count":35,"publisher":"American Society of Hematology","issue":"6","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2001,3,15]]},"abstract":"<jats:p>Interactions between the endothelium and erythrocytes may contribute to the vascular complications of sickle cell disease (SCD). Endothelium-derived nitric oxide (NO) plays a major role in the regulation of vasomotor tone in response to wall shear stress (WSS) variations and pharmacologic stimuli. However, little is known about endothelial NO production in patients with steady-state SCD. We investigated endothelial NO production in response to flow or vasoactive agonists in 16 homozygous patients with steady-state SCD and 15 controls. Flow-mediated dilation (FMD), arterial diameter changes in response to 100% oxygen inhalation, blood viscosity, and calculated WSS were determined in all patients and controls. At baseline, WSS was higher in SCD patients than in controls, whereas arterial diameter was similar. In patients with SCD, FMD was impaired (1.73%\u2009\u00b1\u20090.44% vs 3.97%\u2009\u00b1\u20090.24% in the controls, P\u2009&amp;lt;\u2009.001) and vasoconstriction in response to 100% oxygen was abolished. Using venous occlusion plethysmography, forearm blood flow (FBF) was evaluated in response to acetylcholine, nitro-monomethyl-L-arginine (L-NMMA), and sodium nitroprusside (SNP) in subgroups of 9 controls and 7 patients with SCD. Acetylcholine induced a significantly greater FBF increase in the patients (9.7\u2009\u00b1\u20092.9 mL\/min\/100 mL of forearm volume vs 2.5\u2009\u00b1\u20091.5 mL\/min\/100 mL in the controls,P\u2009&amp;lt;\u2009.001), whereas responses to L-NMMA and SNP were similar. These results suggest that endothelial dysfunction may prevent the arterial diameter of patients with SCD from adapting to chronic or acute shear stress elevations. This may contribute to the pathophysiology of vaso-occlusive crisis in patients with SCD.<\/jats:p>","DOI":"10.1182\/blood.v97.6.1584","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T08:46:47Z","timestamp":1027759607000},"page":"1584-1589","update-policy":"https:\/\/doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":176,"title":["Endothelial dysfunction in patients with sickle cell disease is related to selective impairment of shear stress\u2013mediated vasodilation"],"prefix":"10.1182","volume":"97","author":[{"given":"Laurent","family":"Belhassen","sequence":"first","affiliation":[{"name":"From the Service de Physiologie\u2013Explorations Fonctionnelles and the Centre de la Dre\u0301panocytose, Ho\u0302pital Henri Mondor, APHP et Universite\u0301 Paris XII, France; and Unite\u0301 de Biorhe\u0301ologie, Universite\u0301 Paris VI et LBHP CNRS ESA 7057, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel","family":"Pelle","sequence":"additional","affiliation":[{"name":"From the Service de Physiologie\u2013Explorations Fonctionnelles and the Centre de la Dre\u0301panocytose, Ho\u0302pital Henri Mondor, APHP et Universite\u0301 Paris XII, France; and Unite\u0301 de Biorhe\u0301ologie, Universite\u0301 Paris VI et LBHP CNRS ESA 7057, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sa\u0131\u0308d","family":"Sediame","sequence":"additional","affiliation":[{"name":"From the Service de Physiologie\u2013Explorations Fonctionnelles and the Centre de la Dre\u0301panocytose, Ho\u0302pital Henri Mondor, APHP et Universite\u0301 Paris XII, France; and Unite\u0301 de Biorhe\u0301ologie, Universite\u0301 Paris VI et LBHP CNRS ESA 7057, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dora","family":"Bachir","sequence":"additional","affiliation":[{"name":"From the Service de Physiologie\u2013Explorations Fonctionnelles and the Centre de la Dre\u0301panocytose, Ho\u0302pital Henri Mondor, APHP et Universite\u0301 Paris XII, France; and Unite\u0301 de Biorhe\u0301ologie, Universite\u0301 Paris VI et LBHP CNRS ESA 7057, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudine","family":"Carville","sequence":"additional","affiliation":[{"name":"From the Service de Physiologie\u2013Explorations Fonctionnelles and the Centre de la Dre\u0301panocytose, Ho\u0302pital Henri Mondor, APHP et Universite\u0301 Paris XII, France; and Unite\u0301 de Biorhe\u0301ologie, Universite\u0301 Paris VI et LBHP CNRS ESA 7057, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Bucherer","sequence":"additional","affiliation":[{"name":"From the Service de Physiologie\u2013Explorations Fonctionnelles and the Centre de la Dre\u0301panocytose, Ho\u0302pital Henri Mondor, APHP et Universite\u0301 Paris XII, France; and Unite\u0301 de Biorhe\u0301ologie, Universite\u0301 Paris VI et LBHP CNRS ESA 7057, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Lacombe","sequence":"additional","affiliation":[{"name":"From the Service de Physiologie\u2013Explorations Fonctionnelles and the Centre de la Dre\u0301panocytose, Ho\u0302pital Henri Mondor, APHP et Universite\u0301 Paris XII, France; and Unite\u0301 de Biorhe\u0301ologie, Universite\u0301 Paris VI et LBHP CNRS ESA 7057, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frederic","family":"Galacteros","sequence":"additional","affiliation":[{"name":"From the Service de Physiologie\u2013Explorations Fonctionnelles and the Centre de la Dre\u0301panocytose, Ho\u0302pital Henri Mondor, APHP et Universite\u0301 Paris XII, France; and Unite\u0301 de Biorhe\u0301ologie, Universite\u0301 Paris VI et LBHP CNRS ESA 7057, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Serge","family":"Adnot","sequence":"additional","affiliation":[{"name":"From the Service de Physiologie\u2013Explorations Fonctionnelles and the Centre de la Dre\u0301panocytose, Ho\u0302pital Henri Mondor, APHP et Universite\u0301 Paris XII, France; and Unite\u0301 de Biorhe\u0301ologie, Universite\u0301 Paris VI et LBHP CNRS ESA 7057, France."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"234","reference":[{"key":"2020021220492607000_B1","doi-asserted-by":"crossref","first-page":"1555","DOI":"10.1182\/blood.V94.5.1555","article-title":"Sickle cell acute chest syndrome: pathogenesis and rationale for treatment.","volume":"94","author":"Stuart","year":"1999","journal-title":"Blood."},{"key":"2020021220492607000_B2","first-page":"339","article-title":"Cardiorespiratory adjustments in chronic sickle cell anemia.","volume":"19","author":"Lonsdorfer","year":"1983","journal-title":"Bull Eur 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