{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T13:53:44Z","timestamp":1761486824976},"reference-count":24,"publisher":"American Society of Hematology","issue":"6","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2001,3,15]]},"abstract":"<jats:p>To further characterize hematopoietic \u201creplicative stress\u201d induced by bone marrow transplantation (BMT), the cell-cycle status of CD90+\/\u2212 subsets of marrow CD34+ cells obtained 2 to 6 months after transplantation from 11 fully chimeric recipients was examined. Cycling profiles, derived by flow cytometry after staining with Hoechst 33342 and pyronin Y, were compared with those of 14 healthy marrow donors. Primitive CD34+CD90+cells represented a smaller proportion of CD34+ cells in recipients (10%\u2009\u00b1\u20094% versus 19.6%\u2009\u00b1\u20095.3% in donors;P\u2009&amp;lt;\u2009.0001) and were more mitotically active, with the proportion of cells in S\/G2\/M nearly 4-fold higher than in donors (15.6%\u2009\u00b1\u20093% and 4.4%\u2009\u00b1\u20091.6%, respectively;P\u2009&amp;lt;\u2009.0001). By comparison, there was a modest increase in the proportion of CD34+CD90\u2212progenitors in S\/G2\/M after BMT (10.9%\u2009\u00b1\u20091% vs 9.6%\u2009\u00b1\u20092% in donors; P\u2009=\u2009.04). Replicative stress after BMT is borne predominantly by cells in a diminished CD34+CD90+ population.<\/jats:p>","DOI":"10.1182\/blood.v97.6.1876","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T12:46:47Z","timestamp":1027774007000},"page":"1876-1878","update-policy":"http:\/\/dx.doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":20,"title":["Replicative stress after allogeneic bone marrow transplantation: changes in cycling of CD34+CD90+ and CD34+CD90\u2212 hematopoietic progenitors"],"prefix":"10.1182","volume":"97","author":[{"given":"Ian","family":"Thornley","sequence":"first","affiliation":[{"name":"From the Division of Hematology\/Oncology, The Hospital for Sick Children, and the Department of Medical Oncology and Hematology, University Health Network, University of Toronto, Ontario, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. Robert","family":"Sutherland","sequence":"additional","affiliation":[{"name":"From the Division of Hematology\/Oncology, The Hospital for Sick Children, and the Department of Medical Oncology and Hematology, University Health Network, University of Toronto, Ontario, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rakash","family":"Nayar","sequence":"additional","affiliation":[{"name":"From the Division of Hematology\/Oncology, The Hospital for Sick Children, and the Department of Medical Oncology and Hematology, University Health Network, University of Toronto, Ontario, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lillian","family":"Sung","sequence":"additional","affiliation":[{"name":"From the Division of Hematology\/Oncology, The Hospital for Sick Children, and the Department of Medical Oncology and Hematology, University Health Network, University of Toronto, Ontario, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melvin H.","family":"Freedman","sequence":"additional","affiliation":[{"name":"From the Division of Hematology\/Oncology, The Hospital for Sick Children, and the Department of Medical Oncology and Hematology, University Health Network, University of Toronto, Ontario, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans A.","family":"Messner","sequence":"additional","affiliation":[{"name":"From the Division of Hematology\/Oncology, The Hospital for Sick Children, and the Department of Medical Oncology and Hematology, University Health Network, University of Toronto, Ontario, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"234","reference":[{"key":"2020021223240720600_B1","doi-asserted-by":"crossref","first-page":"1425","DOI":"10.1182\/blood.V70.5.1425.1425","article-title":"Clonogenic hemopoietic precursors in bone marrow transplantation.","volume":"70","author":"Messner","year":"1987","journal-title":"Blood."},{"key":"2020021223240720600_B2","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1111\/j.1365-2141.1987.tb06127.x","article-title":"Haemopoietic reconstitution after allogeneic bone marrow transplantation in man: recovery of haemopoietic progenitors (CFU-Mix, BFU-E and CFU-GM).","volume":"65","author":"Ma","year":"1987","journal-title":"Br J Haematol."},{"key":"2020021223240720600_B3","first-page":"471","article-title":"Alterations in the progenitor cell population follow recovery from myeloablative therapy and bone marrow transplantation.","volume":"25","author":"Novitzky","year":"1997","journal-title":"Exp Hematol."},{"key":"2020021223240720600_B4","doi-asserted-by":"crossref","first-page":"1011","DOI":"10.1038\/sj.bmt.1700785","article-title":"Deficient reconstitution of early progenitors after allogeneic bone marrow transplantation.","volume":"19","author":"Podesta","year":"1997","journal-title":"Bone Marrow Transplant."},{"key":"2020021223240720600_B5","doi-asserted-by":"crossref","first-page":"1029","DOI":"10.1038\/sj.bmt.1701759","article-title":"Long-lasting decrease of marrow and circulating long-term culture initiating cells after allogeneic bone marrow transplant.","volume":"23","author":"Selleri","year":"1999","journal-title":"Bone Marrow Transplant."},{"key":"2020021223240720600_B6","doi-asserted-by":"crossref","first-page":"13782","DOI":"10.1073\/pnas.94.25.13782","article-title":"In vivo telomere dynamics of human hematopoietic stem cells.","volume":"94","author":"Notaro","year":"1997","journal-title":"Proc Natl Acad Sci U S A."},{"key":"2020021223240720600_B7","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1016\/S0140-6736(97)08256-1","article-title":"Accelerated telomere shortening in young recipients of allogeneic bone-marrow transplants.","volume":"351","author":"Wynn","year":"1998","journal-title":"Lancet."},{"key":"2020021223240720600_B8","doi-asserted-by":"crossref","first-page":"997","DOI":"10.1046\/j.1365-2141.1999.01450.x","article-title":"Telomere shortening in leucocyte subsets of long-term survivors of allogeneic bone marrow transplantation.","volume":"105","author":"Wynn","year":"1999","journal-title":"Br J Haematol."},{"key":"2020021223240720600_B9","doi-asserted-by":"crossref","first-page":"730","DOI":"10.1038\/nm0797-730","article-title":"Human acute myeloid leukemia is organized as a hierarchy that originates from a primitive hematopoietic cell.","volume":"3","author":"Bonnet","year":"1997","journal-title":"Nat Med."},{"key":"2020021223240720600_B10","first-page":"818","article-title":"Report on the CD34 cluster workshop.","volume-title":"Leucocyte Typing IV. 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