{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T13:53:43Z","timestamp":1761486823491},"reference-count":22,"publisher":"American Society of Hematology","issue":"8","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2001,4,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>BCL-6 is essential for germinal center formation and thus for affinity maturation of immunoglobulin (Ig) genes by somatic mutations. The 5\u2032-noncoding region of the BCL-6gene is even a target for the mutation machinery. Translocations of theBCL-6 gene to heterologous promoters and mutations of its 5\u2032-noncoding regulatory region were reported to be potential mechanisms for deregulating BCL-6 expression and for playing a role in the genesis of non-Hodgkin lymphoma. In line with this hypothesis is the observation that B-cell lymphoma with somatic mutations, such as diffuse large B-cell lymphoma and follicular lymphoma, also carryBCL-6 mutations, some of which are recurrently detectable. Classic Hodgkin disease (cHD) is also derived from B cells with high loads of somatic mutations and thus a further candidate forBCL-6 mutations. To determine the presence and potential role of BCL-6 mutations in cHD, the 5\u2032-noncodingBCL-6 proportion of single Hodgkin and Reed-Sternberg (HRS) cells from 6 cases of cHD and 6 cases of HD-derived cell lines was analyzed. All B-cell\u2013derived HD cases and cell lines harboredBCL-6 mutations. In contrast, both T-cell\u2013derived HD cases and cell lines were devoid of BCL-6 mutations. With only one exception, there were no lymphoma-specific recurrentBCL-6 mutations detected, and BCL-6 protein was absent from the HRS cells of most cases. In conclusion, (1) somaticBCL-6 mutations are restricted to cHD cases of B-cell origin, and (2) the BCL-6 mutations represent mostly irrelevant somatic base substitutions without consequences for BCL-6 protein expression and the pathogenesis of cHD.<\/jats:p>","DOI":"10.1182\/blood.v97.8.2401","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T08:46:47Z","timestamp":1027759607000},"page":"2401-2405","update-policy":"http:\/\/dx.doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":16,"title":["Analysis of BCL-6 mutations in classic Hodgkin disease of the B- and T-cell type"],"prefix":"10.1182","volume":"97","author":[{"given":"Volkhard","family":"Seitz","sequence":"first","affiliation":[{"name":"From the Institute of Pathology, Consultation and Reference Center for Lymph Node Pathology and Haematopathology, University Hospital Benjamin Franklin, Free University Berlin, Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Hummel","sequence":"additional","affiliation":[{"name":"From the Institute of Pathology, Consultation and Reference Center for Lymph Node Pathology and Haematopathology, University Hospital Benjamin Franklin, Free University Berlin, Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ioannis","family":"Anagnostopoulos","sequence":"additional","affiliation":[{"name":"From the Institute of Pathology, Consultation and Reference Center for Lymph Node Pathology and Haematopathology, University Hospital Benjamin Franklin, Free University Berlin, Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harald","family":"Stein","sequence":"additional","affiliation":[{"name":"From the Institute of Pathology, Consultation and Reference Center for Lymph Node Pathology and Haematopathology, University Hospital Benjamin Franklin, Free University Berlin, Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"234","reference":[{"key":"2020021217583879800_B1","doi-asserted-by":"crossref","first-page":"11816","DOI":"10.1073\/pnas.95.20.11816","article-title":"BCL-6 mutations in normal germinal center B cells: evidence of somatic hypermutation acting outside Ig loci.","volume":"95","author":"Pasqualucci","year":"1998","journal-title":"Proc Natl Acad Sci U S A."},{"key":"2020021217583879800_B2","doi-asserted-by":"crossref","first-page":"1750","DOI":"10.1126\/science.280.5370.1750","article-title":"Mutation of BCL-6 gene in normal B cells by the process of somatic hypermutation of Ig genes.","volume":"280","author":"Shen","year":"1998","journal-title":"Science."},{"key":"2020021217583879800_B3","doi-asserted-by":"crossref","first-page":"12520","DOI":"10.1073\/pnas.92.26.12520","article-title":"Frequent somatic hypermutation of the 5\u2032 noncoding region of the BCL-6 gene in B-cell 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