{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T18:03:13Z","timestamp":1793037793279,"version":"4.5.0"},"reference-count":45,"publisher":"American Society of Hematology","issue":"1","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2001,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Clonality assessment through Southern blot (SB) analysis ofTCRB genes or polymerase chain reaction (PCR) analysis ofTCRG genes is important for diagnosing suspect mature T-cell proliferations. Clonality assessment through reverse transcription (RT)\u2013PCR analysis of V\u03b2-C\u03b2 transcripts and flow cytometry with a V\u03b2 antibody panel covering more than 65% of V\u03b2 domains was validated using 28 SB-defined clonal T-cell receptor (TCR)\u03b1\u03b2+ T-ALL samples and T-cell lines. Next, the diagnostic applicability of the V\u03b2 RT-PCR and flow cytometric clonality assays was studied in 47 mature T-cell proliferations. Clonal V\u03b2-C\u03b2 RT-PCR products were detected in all 47 samples, whereas single V\u03b2 domain usage was found in 31 (66%) of 47 patients. The suspect leukemic cell populations in the other 16 patients showed a complete lack of V\u03b2 monoclonal antibody reactivity that was confirmed by molecular data showing the usage of V\u03b2 gene segments not covered by the applied V\u03b2 monoclonal antibodies. Nevertheless, this could be considered indirect evidence for the \u201cclonal\u201d character of these cells. Remarkably, RT-PCR revealed an oligoclonal pattern in addition to dominant V\u03b2-C\u03b2 products and single V\u03b2 domain expression in many T-LGL proliferations, providing further evidence for the hypothesis raised earlier that T-LGL derive from polyclonal and oligoclonal proliferations of antigen-activated cytotoxic T cells. It is concluded that molecular V\u03b2 analysis serves to assess clonality in suspect T-cell proliferations. However, the faster and cheaper V\u03b2 antibody studies can be used as a powerful screening method for the detection of single V\u03b2 domain expression, followed by molecular studies in patients with more than 20% single V\u03b2 domain expression or large suspect T-cell populations (more than 50%-60%) without V\u03b2 reactivity.<\/jats:p>","DOI":"10.1182\/blood.v98.1.165","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T12:46:47Z","timestamp":1027774007000},"page":"165-173","update-policy":"https:\/\/doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":209,"title":["Molecular and flow cytometric analysis of the V\u03b2 repertoire for clonality assessment in mature TCR\u03b1\u03b2 T-cell proliferations"],"prefix":"10.1182","volume":"98","author":[{"given":"Anton W.","family":"Langerak","sequence":"first","affiliation":[{"name":"From the Department of Immunology, University Hospital Rotterdam\/Erasmus University Rotterdam, The Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rene\u0301","family":"van den Beemd","sequence":"additional","affiliation":[{"name":"From the Department of Immunology, University Hospital Rotterdam\/Erasmus University Rotterdam, The Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingrid L. M.","family":"Wolvers-Tettero","sequence":"additional","affiliation":[{"name":"From the Department of Immunology, University Hospital Rotterdam\/Erasmus University Rotterdam, The Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick P. C.","family":"Boor","sequence":"additional","affiliation":[{"name":"From the Department of Immunology, University Hospital Rotterdam\/Erasmus University Rotterdam, The Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ellen G.","family":"van Lochem","sequence":"additional","affiliation":[{"name":"From the Department of Immunology, University Hospital Rotterdam\/Erasmus University Rotterdam, The Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herbert","family":"Hooijkaas","sequence":"additional","affiliation":[{"name":"From the Department of Immunology, University Hospital Rotterdam\/Erasmus University Rotterdam, The Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacques J. 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