{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T14:51:59Z","timestamp":1772895119195,"version":"3.50.1"},"reference-count":37,"publisher":"American Society of Hematology","issue":"13","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2001,12,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Locus control region (LCR) sequences are involved in the establishment of open chromosomal domains. To evaluate the possibility of exploiting the human CD2 LCR to regulate gene expression by Moloney murine leukemia virus (Mo-MLV)\u2013based retroviral vectors in T cells, it was included in vectors carrying the enhanced green fluorescence protein (EGFP) reporter gene; then transduction in vitro of lymphoid and nonlymphoid cell lines was performed. Deletion of the viral enhancer in the Mo-MLV long terminal repeat was necessary to detect LCR activity in the context of these retroviral vectors. It was found that a full-length (2.1 kb), but not a truncated (1.0 kb), CD2 LCR retained the ability to modulate reporter gene expression by Mo-MLV\u2013derived retroviral vectors, leading to a homogeneous, unimodal pattern of EGFP expression that remained unmodified in culture over time, specifically in T-cell lines; on the other hand, viral titer was strongly reduced compared with vectors not carrying the LCR. Lentiviral vectors containing the CD2 LCR could be generated at higher titers and were used to analyze its effects on gene expression in primary T cells. Subcutaneous implantation of genetically modified cells in immunodeficient mice showed that retroviral vectors carrying the CD2 LCR conferred an advantage in terms of transgene expression in vivo, compared with the parental vector, by preventing the down-modulation of EGFP expression. These findings suggest a potential application of this LCR to increase gene expression by retroviral and lentiviral vectors in T lymphocytes.<\/jats:p>","DOI":"10.1182\/blood.v98.13.3607","type":"journal-article","created":{"date-parts":[[2002,10,28]],"date-time":"2002-10-28T15:44:12Z","timestamp":1035819852000},"page":"3607-3617","update-policy":"https:\/\/doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":22,"title":["Effects of CD2 locus control region sequences on gene expression by retroviral and lentiviral vectors"],"prefix":"10.1182","volume":"98","author":[{"given":"Stefano","family":"Indraccolo","sequence":"first","affiliation":[{"name":"From the IST-Viral and Molecular Oncology Section, the Department of Oncology and Surgical Sciences, and the Department of Pediatrics, University of Padua, Italy; and the Institute of Virology, University of Veterinary Sciences, Vienna, Austria."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sonia","family":"Minuzzo","sequence":"additional","affiliation":[{"name":"From the IST-Viral and Molecular Oncology Section, the Department of Oncology and Surgical Sciences, and the Department of Pediatrics, University of Padua, Italy; and the Institute of Virology, University of Veterinary Sciences, Vienna, Austria."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesca","family":"Roccaforte","sequence":"additional","affiliation":[{"name":"From the IST-Viral and Molecular Oncology Section, the Department of Oncology and Surgical Sciences, and the Department of Pediatrics, University of Padua, Italy; and the Institute of Virology, University of Veterinary Sciences, Vienna, Austria."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rita","family":"Zamarchi","sequence":"additional","affiliation":[{"name":"From the IST-Viral and Molecular Oncology Section, the Department of 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