{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T06:59:36Z","timestamp":1789973976107,"version":"4.0.1"},"reference-count":34,"publisher":"American Society of Hematology","issue":"2","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2001,7,15]]},"abstract":"<jats:p>The human leukemia cell lines K562, CEM, CEM\/VLB100, human leukemic blasts, and the bladder cancer J82 cell line have different sensitivities to UV light\u2013induced apoptosis. It is reported that resistance to UV light\u2013induced apoptosis occurs at a point in the apoptotic pathway upstream of caspase-3 but downstream of mitochondrial cytochrome c release. It is demonstrated that the block is due to deficiency of Apaf-1, a critical member of the apoptosome. Sensitivity to apoptosis was independent of caspase-9b or XIAP (inhibitors of apoptosis proteins) expression or levels of procaspase-9. Transfection of Apaf-1 conferred sensitivity to apoptosis in resistant cells. Apaf-1 deficiency may constitute a significant mode of resistance to apoptosis in human leukemia.<\/jats:p>","DOI":"10.1182\/blood.v98.2.414","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T08:46:47Z","timestamp":1027759607000},"page":"414-421","update-policy":"https:\/\/doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":111,"title":["Apaf-1 protein deficiency confers resistance to cytochromec\u2013dependent apoptosis in human leukemic cells"],"prefix":"10.1182","volume":"98","author":[{"given":"Li","family":"Jia","sequence":"first","affiliation":[{"name":"From the Department of Haematology, St Bartholomew's and The Royal London School of Medicine and Dentistry, London, United Kingdom; and the Department of Microbiology and Immunology, Kimmel Cancer Institute, Thomas Jefferson University, Philadelphia, PA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Srinivasa 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