{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T02:36:15Z","timestamp":1761100575950},"reference-count":41,"publisher":"American Society of Hematology","issue":"3","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2001,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Administration of cyclosporine A (CsA) after autologous stem cell transplantation elicits an autoimmune syndrome with pathology similar to graft-versus-host disease (GVHD). This syndrome, termed autologous GVHD, is associated with the appearance of autoreactive T cells directed at major histocompatibility class (MHC) class II antigens. In the rat model of autologous GVHD, clonal analysis reveals that the effector T cells are highly conserved and recognize a peptide from the invariant chain peptide presented by MHC class II. Although human autologous GVHD effector T cells share a similar phenotypic specificity, clonality of the response in humans has not been determined. To examine the human effector T-cell response, the T-cell repertoire of peripheral blood lymphocytes was assessed by complementarity-determining region 3 (CDR3) size distribution analysis and T-cell clonotype analysis in 26 patients treated with CsA after transplantation. Autologous GVHD developed in 3 of 4 patients with human leukocyte antigen (HLA)-DRB1*0701, and clonal expansions of \u03b2-chain variable region (BV)16+ T cells were shared. Clonal expansions within BV15+ and BV22+ T cells were also detected in 4 of 6 patients with HLA-DRB1*1501 and in 3 of 4 patients with HLA-DRB1*0401, respectively. Sequencing of BV16 cDNA for which the CDR3 size pattern exhibited apparent clone predominance revealed an identical CDR3 peptide sequence in 2 different patients, one with HLA-DRB1*0701 and the other with HLA-DRB1*1502. These findings indicate that the discrete antigen-driven expansion of T cells is involved in autologous GVHD.<\/jats:p>","DOI":"10.1182\/blood.v98.3.868","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T12:46:47Z","timestamp":1027774007000},"page":"868-876","update-policy":"http:\/\/dx.doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":25,"title":["Characterization of the T-cell repertoire in autologous graft-versus-host disease (GVHD): evidence for the involvement of antigen-driven T-cell response in the development of autologous GVHD"],"prefix":"10.1182","volume":"98","author":[{"given":"Yuji","family":"Miura","sequence":"first","affiliation":[{"name":"From the Oncology Center, Johns Hopkins University School of Medicine, Baltimore, MD; the Department of Hematology and Immunology, Ishikawa Prefectural Central Hospital; and the Third Department of Medicine, Kanazawa University School of Medicine, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher J.","family":"Thoburn","sequence":"additional","affiliation":[{"name":"From the Oncology Center, Johns Hopkins University School of Medicine, Baltimore, MD; the Department of Hematology and Immunology, Ishikawa Prefectural Central Hospital; and the Third Department of Medicine, Kanazawa University School of Medicine, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emilie C.","family":"Bright","sequence":"additional","affiliation":[{"name":"From the Oncology Center, Johns Hopkins University School of Medicine, Baltimore, MD; the Department of Hematology and Immunology, Ishikawa Prefectural Central Hospital; and the Third Department of Medicine, Kanazawa University School of Medicine, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthias","family":"Sommer","sequence":"additional","affiliation":[{"name":"From the Oncology Center, Johns Hopkins University School of Medicine, Baltimore, MD; the Department of Hematology and Immunology, Ishikawa Prefectural Central Hospital; and the Third Department of Medicine, Kanazawa University School of Medicine, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan","family":"Lefell","sequence":"additional","affiliation":[{"name":"From the Oncology Center, Johns Hopkins University School of Medicine, Baltimore, MD; the Department of Hematology and Immunology, Ishikawa Prefectural Central Hospital; and the Third Department of Medicine, Kanazawa University School of Medicine, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mikio","family":"Ueda","sequence":"additional","affiliation":[{"name":"From the Oncology Center, Johns Hopkins University School of Medicine, Baltimore, MD; the Department of Hematology and Immunology, Ishikawa Prefectural Central Hospital; and the Third Department of Medicine, Kanazawa University School of Medicine, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shinji","family":"Nakao","sequence":"additional","affiliation":[{"name":"From the Oncology Center, Johns Hopkins University School of Medicine, Baltimore, MD; the Department of Hematology and Immunology, Ishikawa Prefectural Central Hospital; and the Third Department of Medicine, Kanazawa University School of Medicine, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Allan D.","family":"Hess","sequence":"additional","affiliation":[{"name":"From the Oncology Center, Johns Hopkins University School of Medicine, Baltimore, MD; the Department of Hematology and Immunology, Ishikawa Prefectural Central Hospital; and the Third Department of Medicine, Kanazawa University School of Medicine, 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aggressive lymphoma: a pilot study.","volume":"80","author":"Nademanee","year":"1992","journal-title":"Blood."},{"key":"2020021221091246700_B4","doi-asserted-by":"crossref","first-page":"2483","DOI":"10.1200\/JCO.1995.13.10.2483","article-title":"High-dose chemotherapy with hematopoietic rescue as primary treatment for metastatic breast cancer: a randomized trial.","volume":"13","author":"Bezwoda","year":"1995","journal-title":"J Clin Oncol."},{"key":"2020021221091246700_B5","doi-asserted-by":"crossref","first-page":"2543","DOI":"10.1200\/JCO.1994.12.12.2543","article-title":"Comparison of autologous bone marrow transplantation with sequential chemotherapy for intermediate-grade and high-grade non-Hodgkin's lymphoma in first complete remission: a study of 464 patients.","volume":"12","author":"Haioun","year":"1994","journal-title":"J Clin Oncol."},{"key":"2020021221091246700_B6","doi-asserted-by":"crossref","first-page":"1045","DOI":"10.1056\/NEJM199504203321601","article-title":"Comparison of CHOP chemotherapy with autologous bone marrow transplantation for slowly responding patients with aggressive non-Hodgkin's lymphoma.","volume":"332","author":"Verdonck","year":"1995","journal-title":"N Engl J Med."},{"key":"2020021221091246700_B7","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1016\/S0140-6736(98)01350-6","article-title":"Randomised trial of high-dose chemotherapy and haemopoietic progenitor-cell support in operable breast cancer with extensive axillary lymph-node involvement.","volume":"352","author":"Rodenhuis","year":"1998","journal-title":"Lancet."},{"key":"2020021221091246700_B8","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1084\/jem.158.1.1","article-title":"Graft-versus-host disease in cyclosporine treated rats after syngeneic autologous bone marrow reconstitution.","volume":"158","author":"Glazier","year":"1983","journal-title":"J Exp Med."},{"key":"2020021221091246700_B9","doi-asserted-by":"crossref","first-page":"718","DOI":"10.1084\/jem.161.4.718","article-title":"Development of graft-vs.-host disease-like syndrome in cyclosporine-treated rats after syngeneic bone marrow transplantation, I: development of cytotoxic T lymphocytes with apparent polyclonal anti-Ia specificity, including autoreactivity.","volume":"161","author":"Hess","year":"1985","journal-title":"J Exp Med."},{"key":"2020021221091246700_B10","doi-asserted-by":"crossref","first-page":"754","DOI":"10.1016\/S0140-6736(89)92575-0","article-title":"Induction of graft-versus-host disease after autologous bone marrow transplantation.","volume":"333","author":"Jones","year":"1989","journal-title":"Lancet."},{"key":"2020021221091246700_B11","doi-asserted-by":"crossref","first-page":"2061","DOI":"10.1182\/blood.V84.7.2061.2061","article-title":"Graft-versus-host disease: 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cyclosporine-induced syngeneic graft-versus-host disease in the rat.","volume":"74","author":"Geller","year":"1989","journal-title":"Blood."},{"key":"2020021221091246700_B15","doi-asserted-by":"crossref","first-page":"2203","DOI":"10.1182\/blood.V89.6.2203","article-title":"Specificity of effector T lymphocytes in autologous graft-versus-host disease: role of the major histocompatibility complex class II invariant chain peptide.","volume":"89","author":"Hess","year":"1997","journal-title":"Blood."},{"key":"2020021221091246700_B16","doi-asserted-by":"crossref","first-page":"400","DOI":"10.4049\/jimmunol.153.1.400","article-title":"Characterization of peripheral autoregulatory mechanisms that prevent development cyclosporine-induced syngeneic graft-versus-host disease.","volume":"153","author":"Hess","year":"1994","journal-title":"J Immunol."},{"key":"2020021221091246700_B17","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1097\/00007890-199507270-00010","article-title":"In vivo reactivity of T cell clones isolated from mice with syngeneic graft-versus-host disease.","volume":"60","author":"Bryson","year":"1995","journal-title":"Transplant."},{"key":"2020021221091246700_B18","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1097\/00007890-199803270-00004","article-title":"Promiscuous recognition of major histocompatibility complex class II determinants in cyclosporine-induced syngeneic graft-versus-host disease: specificity of cytolytic effector T cells.","volume":"65","author":"Hess","year":"1998","journal-title":"Transplant."},{"key":"2020021221091246700_B19","first-page":"1363","article-title":"Cyclosporine-induced autologous graft-versus-host disease: assessment of cytolytic effector mechanisms and the V\u03b2 T cell receptor repertoire.","volume":"27","author":"Ruvolo","year":"1995","journal-title":"Transplant Proc."},{"key":"2020021221091246700_B20","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1067\/mai.2000.106832","article-title":"Induction of autologous graft-versus-host disease with cyclosporine A after peripheral blood stem cell transplantation: analysis of factors affecting induction.","volume":"106","author":"Miura","year":"2000","journal-title":"J Allergy Clin Immunol."},{"key":"2020021221091246700_B21","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1200\/JCO.1993.11.3.478","article-title":"Phase I trial of intravenous cyclosporine to induce graft-versus-host disease in women undergoing autologous bone marrow transplantation for breast cancer.","volume":"11","author":"Kennedy","year":"1993","journal-title":"J Clin Oncol."},{"key":"2020021221091246700_B22","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/0167-5699(95)80117-0","article-title":"T-cell repertoire diversity and clonal expansion in normal and clinical 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Med."},{"key":"2020021221091246700_B35","doi-asserted-by":"crossref","first-page":"9703","DOI":"10.1073\/pnas.90.20.9703","article-title":"The segment of invariant chain that is critical for association with major histocompatibility complex class II molecules contains the sequence of a peptide eluted from class II polypeptides.","volume":"90","author":"Freisewinkel","year":"1993","journal-title":"Proc Natl Acad Sci U S A."},{"key":"2020021221091246700_B36","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1146\/annurev.iy.12.040194.001355","article-title":"Assembly, transport and function of MHC class II molecules.","volume":"12","author":"Cresswell","year":"1994","journal-title":"Annu Rev Immunol."},{"key":"2020021221091246700_B37","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1084\/jem.179.1.63","article-title":"Rat T cell response to superantigens, II: allelic differences in V\u03b2 8.2 and V\u03b2 8.5 \u03b2 chains determine responsiveness to staphylococcal enterotoxin B and mouse mammary tumor virus-encoded proteins.","volume":"179","author":"Gold","year":"1994","journal-title":"J Exp Med."},{"key":"2020021221091246700_B38","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1111\/j.1365-3083.1994.tb03390.x","article-title":"Interactions between staphylococcal superantigens and human T-cell clones are predominantly but not exclusively governed by their T-cell receptor V\u03b2 usage.","volume":"38","author":"Lundin","year":"1994","journal-title":"Scand J Immunol."},{"key":"2020021221091246700_B39","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1111\/j.1749-6632.1995.tb44493.x","article-title":"Bacterial toxin superantigens stimulate all members of susceptible VB gene families.","volume":"756","author":"Yassai","year":"1995","journal-title":"Ann N Y Acad Sci."},{"key":"2020021221091246700_B40","doi-asserted-by":"crossref","first-page":"1847","DOI":"10.1084\/jem.181.5.1847","article-title":"Peptide binding specificity of HLA-DR4 molecules: correlation with rheumatoid arthritis association.","volume":"181","author":"Hammer","year":"1995","journal-title":"J Exp Med."},{"key":"2020021221091246700_B41","doi-asserted-by":"crossref","first-page":"3008","DOI":"10.1182\/blood.V93.9.3008","article-title":"Characterization of T-cell repertoire of the bone marrow in immune-mediated aplastic anemia: evidence for the involvement of antigen-driven T-cell response in cyclosporine-dependent aplastic 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