{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T20:30:04Z","timestamp":1771360204721,"version":"3.50.1"},"reference-count":47,"publisher":"American Society of Hematology","issue":"7","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2001,10,1]]},"abstract":"<jats:p>HVMNE is a novel Epstein-Barr (EBV)\u2013like virus isolated from a Macaca nemestrina with CD8+ T-cell mycosis fungoides\u2013cutaneous T-cell lymphoma. Here it is demonstrated that intravenous inoculation of irradiated HVMNE-infected T cells or cell-free virus from the J94356PBMC cell line in New Zealand White rabbits results in seroconversion to the viral capsid antigen (VCA) of EBV; all animals that seroconverted to VCA developed malignant lymphoma within months of inoculation. In contrast, control rabbits, inoculated with heat-inactivated culture supernatants from the same cell line, failed to seroconvert to VCA and did not develop disease. Disseminated lymphoma cells of mixed origin were detected in most vital organs, including the spleen, liver, lungs, kidneys, and heart of the affected rabbits. Neoplastic infiltrates were also observed in lymph nodes, thymus, skin, and subcutaneous tissues. HVMNE DNA and EBV-like RNA expression was demonstrated in the lymphomatous organs and in 2 transformed T-cell lines, one established from the lymph node and the other from the blood of the 2 lymphomatous animals. Analysis of one of these T-cell lines demonstrated the persistence of HVMNEDNA, expression of an LMP1-like protein, and acquisition of interleukin-2 independence, and constitutive activation of the Jak\/STAT pathway. Thus, HVMNE in rabbits provides a valuable animal model for human T-cell lymphoma whereby genetic determinants for T-cell transformation by this EBV-like animal virus can be studied.<\/jats:p>","DOI":"10.1182\/blood.v98.7.2193","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T12:46:47Z","timestamp":1027774007000},"page":"2193-2199","update-policy":"https:\/\/doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":12,"title":["HVMNE, a novel lymphocryptovirus related to Epstein-Barr virus, induces lymphoma in New Zealand White rabbits"],"prefix":"10.1182","volume":"98","author":[{"given":"Maria G.","family":"Ferrari","sequence":"first","affiliation":[{"name":"From the Basic Research Laboratory, National Cancer Institute, Bethesda, MD; the Leukaemia Research Fund Virus Centre, Glasgow, United Kingdom; and the Advanced Bioscience Laboratories, Kensington, 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lymphoma.","volume":"i","author":"Epstein","year":"1964","journal-title":"Lancet."},{"key":"2020021215571904200_B4","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1128\/CMR.1.3.300","article-title":"Epstein-Barr virus and human diseases: recent advances in diagnosis.","volume":"1","author":"Okano","year":"1988","journal-title":"Clin Microbiol Rev."},{"key":"2020021215571904200_B5","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1073\/pnas.59.1.94","article-title":"Relation of Burkitt's tumor-associated herpes-type virus to infectious mononucleosis.","volume":"59","author":"Henle","year":"1968","journal-title":"Proc Natl Acad Sci U S A."},{"key":"2020021215571904200_B6","doi-asserted-by":"crossref","first-page":"3079","DOI":"10.1182\/blood.V91.8.3079.3079_3079_3083","article-title":"Risks of developing Epstein-Barr virus-related lymphoproliferative disorders after T-cell\u2013depleted marrow transplants: CAMPATH users.","volume":"91","author":"Hale","year":"1998","journal-title":"Blood."},{"key":"2020021215571904200_B7","doi-asserted-by":"crossref","first-page":"1056","DOI":"10.1038\/2281056a0","article-title":"EBV DNA in biopsies of Burkitt tumours and anaplastic carcinomas of the nasopharynx.","volume":"228","author":"zur Hausen","year":"1970","journal-title":"Nature."},{"key":"2020021215571904200_B8","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1002\/ijc.2910120202","article-title":"Pilot experiments with EB virus in owl monkey (Aotus trivirgatus), I: reticuloproliferative disease in an inoculated animal.","volume":"12","author":"Epstein","year":"1973","journal-title":"Int J Cancer."},{"key":"2020021215571904200_B9","doi-asserted-by":"crossref","first-page":"S5","DOI":"10.1093\/annonc\/9.suppl_5.S5","article-title":"Epstein-Barr virus in Hodgkin's disease.","volume":"9","author":"Chapman","year":"1998","journal-title":"Ann Oncol."},{"key":"2020021215571904200_B10","doi-asserted-by":"crossref","first-page":"848","DOI":"10.1097\/00007890-199709270-00010","article-title":"Reduced incidence of Epstein-Barr virus-associated posttransplant lymphoproliferative disorder using preemptive antiviral therapy.","volume":"64","author":"Darenkov","year":"1997","journal-title":"Transplantation."},{"key":"2020021215571904200_B11","doi-asserted-by":"crossref","first-page":"1604","DOI":"10.1097\/00007890-199812270-00006","article-title":"Prevention and preemptive therapy of postransplant lymphoproliferative disease in pediatric liver recipients.","volume":"66","author":"McDiarmid","year":"1998","journal-title":"Transplantation."},{"key":"2020021215571904200_B12","first-page":"2343","article-title":"Epstein-Barr virus and its replication.","volume":"2","author":"Kieff","year":"1996","journal-title":"Fields Virology."},{"key":"2020021215571904200_B13","first-page":"379","article-title":"Cytotoxic T-cell lymphoma diffusely 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infection.","volume":"77","author":"Donadieu","year":"1996","journal-title":"Cancer."},{"key":"2020021215571904200_B16","doi-asserted-by":"crossref","first-page":"355","DOI":"10.2340\/0001555574359360","article-title":"Presence of Epstein-Barr virus in cutaneous lesions of mycosis fungoides and Se\u0301zary syndrome.","volume":"74","author":"Dreno","year":"1994","journal-title":"Acta Derm Venereol."},{"key":"2020021215571904200_B17","doi-asserted-by":"crossref","first-page":"2085","DOI":"10.1182\/blood.V91.6.2085","article-title":"A syndrome of peripheral blood T-cell infection with Epstein-Barr virus (EBV) followed by EBV-positive T-cell lymphoma.","volume":"91","author":"Kanegane","year":"1998","journal-title":"Blood."},{"key":"2020021215571904200_B18","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1046\/j.1365-2141.1998.00556.x","article-title":"t(3;17)(q21;q25) In Epstein-Barr virus associated peripheral T-cell lymphoma: a paediatric case.","volume":"100","author":"Millot","year":"1998","journal-title":"Br J Haematol."},{"key":"2020021215571904200_B19","first-page":"1595","article-title":"Disseminated nasal T cell lymphoma with a partial deletion of the LMP1 oncogene in a patient with chronic active Epstein-Barr virus infection.","volume":"9","author":"Minegishi","year":"1995","journal-title":"Leukemia."},{"key":"2020021215571904200_B20","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1128\/CMR.4.1.129","article-title":"Severe chronic active Epstein-Barr virus infection syndrome.","volume":"4","author":"Okano","year":"1991","journal-title":"Clin Microbiol Rev."},{"key":"2020021215571904200_B21","first-page":"1093","article-title":"Epstein-Barr virus in a CD8-positive T-cell lymphoma.","volume":"136","author":"Richel","year":"1990","journal-title":"Am J Pathol."},{"key":"2020021215571904200_B22","doi-asserted-by":"crossref","first-page":"47","DOI":"10.3109\/10428199209053601","article-title":"Clinicopathological spectrum of Epstein-Barr virus-associated T cell malignancies.","volume":"7","author":"Su","year":"1992","journal-title":"Leuk Lymphoma."},{"key":"2020021215571904200_B23","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1182\/blood.V85.2.480.480","article-title":"Lack of the expression of EBNA-2 and LMP-1 in T-cell neoplasms possessing Epstein-Barr virus.","volume":"85","author":"Suzushima","year":"1995","journal-title":"Blood."},{"key":"2020021215571904200_B24","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1097\/00000478-199406000-00006","article-title":"Angiocentric immunoproliferative lesion associated with chronic active Epstein-Barr virus infection in an 11-year-old boy: clonotopic proliferation of Epstein-Barr virus-bearing CD4+ T lymphocytes.","volume":"18","author":"Tanaka","year":"1994","journal-title":"Am J Surg Pathol."},{"key":"2020021215571904200_B25","doi-asserted-by":"crossref","first-page":"1123","DOI":"10.1002\/eji.1830220504","article-title":"Interaction between Epstein-Barr virus and a T cell line (HSB-2) via a receptor phenotypically distinct from complement receptor type 2.","volume":"22","author":"Hedrick","year":"1992","journal-title":"Eur J Immunol."},{"key":"2020021215571904200_B26","first-page":"3171","article-title":"In situ RT-PCR detection of Epstein-Barr virus immediate-early transcripts in CD4+ and CD8+ T lymphocytes.","volume":"18","author":"Guan","year":"1998","journal-title":"Anticancer Res."},{"key":"2020021215571904200_B27","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1056\/NEJM198803243181203","article-title":"T-cell lymphomas containing Epstein-Barr viral DNA in patients with chronic Epstein-Barr virus infections.","volume":"318","author":"Jones","year":"1988","journal-title":"N Engl J Med."},{"key":"2020021215571904200_B28","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1016\/S0046-8177(84)80194-X","article-title":"Virus-associated hemophagocytic syndrome due to Epstein-Barr virus.","volume":"15","author":"Reisman","year":"1984","journal-title":"Hum Pathol."},{"key":"2020021215571904200_B29","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1093\/ajcp\/106.1.69","article-title":"Angiocentric immunoproliferative lesions of the lymph node.","volume":"106","author":"Takeshita","year":"1996","journal-title":"Am J Clin Pathol."},{"key":"2020021215571904200_B30","doi-asserted-by":"crossref","first-page":"1612","DOI":"10.1182\/blood.V83.6.1612.1612","article-title":"Presence of Epstein-Barr virus in extranodal T-cell lymphomas: differences in relation to site.","volume":"83","author":"de Bruin","year":"1994","journal-title":"Blood."},{"key":"2020021215571904200_B31","doi-asserted-by":"crossref","first-page":"1081","DOI":"10.1001\/archderm.1997.03890450023003","article-title":"Clinicopathologic manifestations of Epstein-Barr virus-associated cutaneous lymphoproliferative disorders.","volume":"133","author":"Iwatsuki","year":"1997","journal-title":"Arch Dermatol."},{"key":"2020021215571904200_B32","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/path.1711650102","article-title":"Epstein-Barr virus: an aetiological factor in cutaneous lymphoproliferative disorders?","volume":"165","author":"Slater","year":"1991","journal-title":"J Pathol."},{"key":"2020021215571904200_B33","first-page":"195","article-title":"Studies of HIV infection and the development of Epstein-Barr virus-related B cell lymphomas following transfer of human lymphocytes to mice with severe combined immunodeficiency.","volume":"152","author":"Mosier","year":"1989","journal-title":"Curr Top Microbiol Immunol."},{"key":"2020021215571904200_B34","doi-asserted-by":"crossref","first-page":"798","DOI":"10.1002\/ijc.2910180611","article-title":"Properties of a baboon lymphotropic herpesvirus related to Epstein-Barr virus.","volume":"18","author":"Falk","year":"1976","journal-title":"Int J Cancer."},{"key":"2020021215571904200_B35","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1073\/pnas.58.2.478","article-title":"Complement-fixing antibodies in sera of human and non-human primates to viral antigens derived from Burkitt's lymphoma cells.","volume":"58","author":"Gerber","year":"1967","journal-title":"Proc Natl Acad Sci U S A."},{"key":"2020021215571904200_B36","doi-asserted-by":"crossref","first-page":"1090","DOI":"10.1128\/JVI.19.3.1090-1099.1976","article-title":"Antigens and DNA of a chimpanzee agent related to Epstein-Barr virus.","volume":"19","author":"Gerber","year":"1976","journal-title":"J Virol."},{"key":"2020021215571904200_B37","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1002\/ijc.2910200318","article-title":"Biologic and antigenic characteristics of Epstein-Barr virus-related herpes-viruses of chimpanzees and baboons.","volume":"20","author":"Gerber","year":"1977","journal-title":"Int J Cancer."},{"key":"2020021215571904200_B38","doi-asserted-by":"crossref","first-page":"2090","DOI":"10.1182\/blood.V94.6.2090","article-title":"A novel Epstein-Barr virus-like virus, HV(MNE), in a Macaca nemestrina with mycosis fungoides.","volume":"94","author":"Rivadeneira","year":"1999","journal-title":"Blood."},{"key":"2020021215571904200_B39","doi-asserted-by":"crossref","first-page":"872","DOI":"10.1002\/ijc.2910630620","article-title":"Malignant lymphoma induction in rabbits by intravenous inoculation of Epstein-Barr virus-related herpesvirus from HTLV-II\u2013transformed cynomolgus leukocyte cell line (Si-IIA).","volume":"63","author":"Hayashi","year":"1995","journal-title":"Int J Cancer."},{"key":"2020021215571904200_B40","doi-asserted-by":"crossref","first-page":"1979","DOI":"10.1007\/BF01322687","article-title":"Malignant lymphomas induced by an Epstein-Barr virus-related herpesvirus from Macaca arctoides: a rabbit model.","volume":"140","author":"Wutzler","year":"1995","journal-title":"Arch Virol."},{"key":"2020021215571904200_B41","doi-asserted-by":"crossref","first-page":"4138","DOI":"10.1128\/JVI.71.5.4138-4144.1997","article-title":"Identification of two homologs of the Kaposi's sarcoma-associated herpesvirus (human herpesvirus 8) in retroperitoneal fibromatosis of different macaque species.","volume":"71","author":"Rose","year":"1997","journal-title":"J Virol."},{"key":"2020021215571904200_B42","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1126\/science.7604283","article-title":"Constitutively activated JAK-STAT pathway in T-cells transformed with HTLV-I.","volume":"269","author":"Migone","year":"1995","journal-title":"Science."},{"key":"2020021215571904200_B43","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1182\/blood.V88.3.809.809","article-title":"Constitutive activation of STAT proteins in primary lymphoid and myeloid leukemia cells and in Epstein-Barr virus (EBV)\u2013related lymphoma cell lines.","volume":"88","author":"Weber-Nordt","year":"1996","journal-title":"Blood."},{"key":"2020021215571904200_B44","doi-asserted-by":"crossref","first-page":"1548","DOI":"10.1172\/JCI118193","article-title":"Constitutive activation of different Jak tyrosine kinases in human T-cell leukemia virus type 1 (HTLV-1) tax protein or virus-transformed cells.","volume":"96","author":"Xu","year":"1995","journal-title":"J Clin Invest."},{"key":"2020021215571904200_B45","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1002\/ijc.2910590223","article-title":"DNA of lymphoma-associated herpesvirus (HVMF1) in SIV-infected monkeys 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