{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T01:09:45Z","timestamp":1793149785088,"version":"4.5.0"},"reference-count":27,"publisher":"American Society of Hematology","issue":"11","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2002,6,1]]},"abstract":"<jats:p>We adoptively transferred donor-derived cytomegalovirus (CMV)-specific T-cell lines into 8 stem cell transplant recipients lacking CMV-specific T-cell proliferation. All patients, of whom one was infected by a CMV strain that was genotypically ganciclovir resistant, had received unsuccessful antiviral chemotherapy for more than 4 weeks. CMV-specific lines had been prepared by repetitive stimulation with CMV antigen, which increased the percentage of CMV-specific T cells and ablated alloreactivity completely even against patients mismatched for 1 to 3 HLA antigens. After transfer of 107 T cells\/m2 at a median of 120 days (range, 79-479 days) after transplantation, no side effects were noticed. Despite cessation of antiviral chemotherapy, the CMV load dropped significantly in all 7 evaluable patients, with a maximal reduction after a median of 20 days (range, 5-31 days). In 2 patients with high virus load, the antiviral effect was only transient. One of these patients received a second T-cell infusion, which cleared the virus completely. At a median of 11 days after transfer, CMV-specific T-cell proliferation was demonstrated in 6 patients, and an increase in CMV-specific CD4+ T cells was demonstrated in 5 patients. In 6 patients, 1.12 to 41 CMV-specific CD8+ T cells\/\u03bcL blood were detected at a median of 13 days after transfer, with an increase in all patients lacking CMV-specific CD8+ T cells prior to transfer. Hence, anti-CMV cellular therapy was successful in 5 of 7 patients, whereas in 2 of 7 patients, who received an intensified immune suppression at the time of or after T-cell therapy, only transient reductions in virus load were obtained.<\/jats:p>","DOI":"10.1182\/blood.v99.11.3916","type":"journal-article","created":{"date-parts":[[2002,10,1]],"date-time":"2002-10-01T17:48:18Z","timestamp":1033494498000},"page":"3916-3922","update-policy":"https:\/\/doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":600,"title":["Infusion of cytomegalovirus (CMV)\u2013specific T cells for the treatment of CMV infection not responding to antiviral chemotherapy"],"prefix":"10.1182","volume":"99","author":[{"given":"Hermann","family":"Einsele","sequence":"first","affiliation":[{"name":"From the Medizinische Klinik und Poliklinik, Abteilung II, Tu\u0308bingen, Germany; Division of Immunology and Allergology, University of Geneva, Switzerland; National Center for Competence and Research Molecular Oncology, Swiss Institut for Experimental Cancer Research, Epalinges, Switzerland; Abteilung Medizinische Virologie und Epidemiologie der Viruskrankheiten, Tu\u0308bingen, Germany; and Institut fu\u0308r Zellbiologie Universita\u0308t Tu\u0308bingen, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eddy","family":"Roosnek","sequence":"additional","affiliation":[{"name":"From the Medizinische Klinik und Poliklinik, Abteilung II, Tu\u0308bingen, Germany; Division of Immunology and Allergology, University of Geneva, Switzerland; National Center for Competence and Research Molecular Oncology, Swiss Institut for Experimental Cancer Research, Epalinges, Switzerland; Abteilung Medizinische Virologie und Epidemiologie der Viruskrankheiten, Tu\u0308bingen, Germany; and Institut fu\u0308r Zellbiologie Universita\u0308t Tu\u0308bingen, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Rufer","sequence":"additional","affiliation":[{"name":"From the Medizinische Klinik und Poliklinik, Abteilung II, Tu\u0308bingen, Germany; Division of Immunology and Allergology, University of Geneva, Switzerland; National Center for Competence and Research Molecular Oncology, Swiss Institut for Experimental Cancer Research, Epalinges, Switzerland; Abteilung Medizinische Virologie und Epidemiologie der Viruskrankheiten, Tu\u0308bingen, Germany; and Institut fu\u0308r Zellbiologie Universita\u0308t Tu\u0308bingen, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Sinzger","sequence":"additional","affiliation":[{"name":"From the Medizinische Klinik und Poliklinik, Abteilung II, Tu\u0308bingen, Germany; Division of Immunology and Allergology, University of Geneva, Switzerland; National Center for Competence and Research Molecular Oncology, Swiss Institut for Experimental Cancer Research, Epalinges, Switzerland; Abteilung Medizinische Virologie und Epidemiologie der Viruskrankheiten, Tu\u0308bingen, Germany; and Institut fu\u0308r Zellbiologie Universita\u0308t Tu\u0308bingen, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanne","family":"Riegler","sequence":"additional","affiliation":[{"name":"From the Medizinische Klinik und Poliklinik, Abteilung II, Tu\u0308bingen, Germany; Division of Immunology and Allergology, University of Geneva, Switzerland; National Center for Competence and Research Molecular Oncology, Swiss Institut for Experimental Cancer Research, Epalinges, Switzerland; Abteilung Medizinische Virologie und Epidemiologie der Viruskrankheiten, Tu\u0308bingen, Germany; and Institut fu\u0308r Zellbiologie Universita\u0308t Tu\u0308bingen, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ju\u0308rgen","family":"Lo\u0308ffler","sequence":"additional","affiliation":[{"name":"From the Medizinische Klinik und Poliklinik, Abteilung II, Tu\u0308bingen, Germany; Division of Immunology and Allergology, University of Geneva, Switzerland; National Center for Competence and Research Molecular Oncology, Swiss Institut for Experimental Cancer Research, Epalinges, Switzerland; Abteilung Medizinische Virologie und Epidemiologie der Viruskrankheiten, Tu\u0308bingen, Germany; and Institut fu\u0308r Zellbiologie Universita\u0308t Tu\u0308bingen, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrich","family":"Grigoleit","sequence":"additional","affiliation":[{"name":"From the Medizinische Klinik und Poliklinik, Abteilung II, Tu\u0308bingen, Germany; Division of Immunology and Allergology, University of Geneva, Switzerland; National Center for Competence and Research Molecular Oncology, Swiss Institut for Experimental Cancer Research, Epalinges, Switzerland; Abteilung Medizinische Virologie und Epidemiologie der Viruskrankheiten, Tu\u0308bingen, Germany; and Institut fu\u0308r Zellbiologie Universita\u0308t Tu\u0308bingen, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arnaud","family":"Moris","sequence":"additional","affiliation":[{"name":"From the Medizinische Klinik und Poliklinik, Abteilung II, Tu\u0308bingen, Germany; Division of Immunology and Allergology, University of Geneva, Switzerland; National Center for Competence and Research Molecular Oncology, Swiss Institut for Experimental Cancer Research, Epalinges, Switzerland; Abteilung Medizinische Virologie und Epidemiologie der Viruskrankheiten, Tu\u0308bingen, Germany; and Institut fu\u0308r Zellbiologie Universita\u0308t Tu\u0308bingen, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans-Georg","family":"Rammensee","sequence":"additional","affiliation":[{"name":"From the Medizinische Klinik und Poliklinik, Abteilung II, Tu\u0308bingen, Germany; Division of Immunology and Allergology, University of Geneva, Switzerland; National Center for Competence and Research Molecular Oncology, Swiss Institut for Experimental Cancer Research, Epalinges, Switzerland; Abteilung Medizinische Virologie und Epidemiologie der Viruskrankheiten, Tu\u0308bingen, Germany; and Institut fu\u0308r Zellbiologie Universita\u0308t Tu\u0308bingen, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lothar","family":"Kanz","sequence":"additional","affiliation":[{"name":"From the Medizinische Klinik und Poliklinik, Abteilung II, Tu\u0308bingen, Germany; Division of Immunology and Allergology, University of Geneva, Switzerland; National Center for Competence and Research Molecular Oncology, Swiss Institut for Experimental Cancer Research, Epalinges, Switzerland; Abteilung Medizinische Virologie und Epidemiologie der Viruskrankheiten, Tu\u0308bingen, Germany; and Institut fu\u0308r Zellbiologie Universita\u0308t Tu\u0308bingen, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annette","family":"Kleihauer","sequence":"additional","affiliation":[{"name":"From the Medizinische Klinik und Poliklinik, Abteilung II, Tu\u0308bingen, Germany; Division of Immunology and Allergology, University of Geneva, Switzerland; National Center for Competence and Research Molecular Oncology, Swiss Institut for Experimental Cancer Research, Epalinges, Switzerland; Abteilung Medizinische Virologie und Epidemiologie der Viruskrankheiten, Tu\u0308bingen, Germany; and Institut fu\u0308r Zellbiologie Universita\u0308t Tu\u0308bingen, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Friederike","family":"Frank","sequence":"additional","affiliation":[{"name":"From the Medizinische Klinik und Poliklinik, Abteilung II, Tu\u0308bingen, Germany; Division of Immunology and Allergology, University of Geneva, Switzerland; National Center for Competence and Research Molecular Oncology, Swiss Institut for Experimental Cancer Research, Epalinges, Switzerland; Abteilung Medizinische Virologie und Epidemiologie der Viruskrankheiten, Tu\u0308bingen, Germany; and Institut fu\u0308r Zellbiologie Universita\u0308t Tu\u0308bingen, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerhard","family":"Jahn","sequence":"additional","affiliation":[{"name":"From the Medizinische Klinik und Poliklinik, Abteilung II, Tu\u0308bingen, Germany; Division of Immunology and Allergology, University of Geneva, Switzerland; National Center for Competence and Research Molecular Oncology, Swiss Institut for Experimental Cancer Research, Epalinges, Switzerland; Abteilung Medizinische Virologie und Epidemiologie der Viruskrankheiten, Tu\u0308bingen, Germany; and Institut fu\u0308r Zellbiologie Universita\u0308t Tu\u0308bingen, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Holger","family":"Hebart","sequence":"additional","affiliation":[{"name":"From the Medizinische Klinik und Poliklinik, Abteilung II, Tu\u0308bingen, Germany; Division of Immunology and Allergology, University of Geneva, Switzerland; National Center for Competence and Research Molecular Oncology, Swiss Institut for Experimental Cancer Research, Epalinges, Switzerland; Abteilung Medizinische Virologie und Epidemiologie der Viruskrankheiten, Tu\u0308bingen, Germany; and Institut fu\u0308r Zellbiologie Universita\u0308t Tu\u0308bingen, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"234","reference":[{"key":"2020021219355746600_B1","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1093\/clinids\/14.4.831","article-title":"Treatment of interstitial pneumonitis due to cytomegalovirus with ganciclovir and intravenous immune globulin: experience of European Bone Marrow Transplant Group.","volume":"14","author":"Ljungman","year":"1992","journal-title":"Clin Infect Dis."},{"key":"2020021219355746600_B2","doi-asserted-by":"crossref","first-page":"1111","DOI":"10.1038\/sj.bmt.1700801","article-title":"Screening for CMV-specific T cell proliferation to identify patients at risk of developing late onset CMV disease.","volume":"19","author":"Krause","year":"1997","journal-title":"Bone Marrow Transplant."},{"key":"2020021219355746600_B3","doi-asserted-by":"crossref","first-page":"1971","DOI":"10.1182\/blood.V83.7.1971.1971","article-title":"Recovery of HLA-restricted cytomegalovirus (CMV)-specific T cell responses after allogeneic bone marrow transplant: correlation with CMV disease and effect of ganciclovir prophylaxis.","volume":"83","author":"Li","year":"1994","journal-title":"Blood."},{"key":"2020021219355746600_B4","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1038\/sj.bmt.1702226","article-title":"Risk factors for treatment failures in patients receiving PCR-based preemptive antiviral therapy for CMV infection.","volume":"25","author":"Einsele","year":"2000","journal-title":"Bone Marrow Transplant."},{"key":"2020021219355746600_B5","doi-asserted-by":"crossref","first-page":"782","DOI":"10.1086\/517301","article-title":"Late cytomegalovirus disease in marrow transplantation is predicted by virus load in plasma.","volume":"176","author":"Zaia","year":"1997","journal-title":"J Infect Dis."},{"key":"2020021219355746600_B6","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1056\/NEJM198207013070102","article-title":"Cytotoxic T cells in cytomegalovirus infection. 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