{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T18:53:48Z","timestamp":1791140028228,"version":"4.1.0"},"reference-count":38,"publisher":"American Society of Hematology","issue":"5","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2002,3,1]]},"abstract":"<jats:p>Erythropoiesis results from the proliferation and differentiation of pluripotent stem cells into immature erythroid progenitors (ie, erythroid burst-forming units (BFU-Es), whose growth, survival, and terminal differentiation depends on erythropoietin (Epo). Ineffective erythropoiesis is a common feature of myelodysplastic syndromes (MDS). We used a 2-step liquid-culture procedure to study erythropoiesis in MDS. CD34+ cells from the marrow of patients with MDS were cultured for 10 days in serum-containing medium with Epo, stem cell factor, insulinlike growth factor 1, and steroid hormones until they reached the proerythroblast stage. The cells were then placed in medium containing Epo and insulin for terminal erythroid differentiation. Numbers of both MDS and normal control cells increased 103fold by day 15. However, in semisolid culture, cells from patients with refractory anemia (RA) with ringed sideroblasts and RA or RA with excess of blasts produced significantly fewer BFU-Es than cells from controls. Fluorescence in situ hybridization analysis of interphase nuclei from patients with chromosomal defects indicated that abnormal clones were expanded in vitro. Epo-signaling pathways (STAT5, Akt, and ERK 1\/2) were normally activated in MDS erythroid progenitors. In contrast, apoptosis was significantly increased in MDS cells once they differentiated, whereas it remained low in normal cells. Fas was overexpressed on freshly isolated MDS CD34+ cells and on MDS erythroid cells throughout the culture. Apoptosis coincided with overproduction of Fas ligand during the differentiation stage and was inhibited by Fas-Fc chimeric protein. Thus, MDS CD34+-derived erythroid progenitors proliferated normally in our 2-step liquid culture with Epo but underwent abnormal Fas-dependent apoptosis during differentiation that could be responsible for the impaired erythropoiesis.<\/jats:p>","DOI":"10.1182\/blood.v99.5.1594","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T08:46:40Z","timestamp":1027759600000},"page":"1594-1601","update-policy":"https:\/\/doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":124,"title":["In vitro proliferation and differentiation of erythroid progenitors from patients with myelodysplastic syndromes: evidence for Fas-dependent apoptosis"],"prefix":"10.1182","volume":"99","author":[{"given":"Yann-Erick","family":"Claessens","sequence":"first","affiliation":[{"name":"From the Department of Hematology, AP-HP, Ho\u0302pital Cochin; INSERM U363, Institut Cochin de Ge\u0301ne\u0301tique Mole\u0301culaire, Universite\u0301 Rene\u0301-Descartes, Paris V; and the Laboratory of Histology Embryology and Cytogenetics, AP-HP, Ho\u0302pital Cochin, Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Didier","family":"Bouscary","sequence":"additional","affiliation":[{"name":"From the Department of Hematology, AP-HP, Ho\u0302pital Cochin; INSERM U363, Institut Cochin de Ge\u0301ne\u0301tique Mole\u0301culaire, Universite\u0301 Rene\u0301-Descartes, Paris V; and the Laboratory of Histology Embryology and Cytogenetics, AP-HP, Ho\u0302pital Cochin, Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Michel","family":"Dupont","sequence":"additional","affiliation":[{"name":"From the Department of Hematology, AP-HP, Ho\u0302pital Cochin; INSERM U363, Institut Cochin de Ge\u0301ne\u0301tique Mole\u0301culaire, Universite\u0301 Rene\u0301-Descartes, Paris V; and the Laboratory of Histology Embryology and Cytogenetics, AP-HP, Ho\u0302pital Cochin, Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franc\u0327oise","family":"Picard","sequence":"additional","affiliation":[{"name":"From the Department of Hematology, AP-HP, Ho\u0302pital Cochin; INSERM U363, Institut Cochin de Ge\u0301ne\u0301tique Mole\u0301culaire, Universite\u0301 Rene\u0301-Descartes, Paris V; and the Laboratory of Histology Embryology and Cytogenetics, AP-HP, Ho\u0302pital Cochin, Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josiane","family":"Melle","sequence":"additional","affiliation":[{"name":"From the Department of Hematology, AP-HP, Ho\u0302pital Cochin; INSERM U363, Institut Cochin de Ge\u0301ne\u0301tique Mole\u0301culaire, Universite\u0301 Rene\u0301-Descartes, Paris V; and the Laboratory of Histology Embryology and Cytogenetics, AP-HP, Ho\u0302pital Cochin, Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvie","family":"Gisselbrecht","sequence":"additional","affiliation":[{"name":"From the Department of Hematology, AP-HP, Ho\u0302pital Cochin; INSERM U363, Institut Cochin de Ge\u0301ne\u0301tique Mole\u0301culaire, Universite\u0301 Rene\u0301-Descartes, Paris V; and the Laboratory of Histology Embryology and Cytogenetics, AP-HP, Ho\u0302pital Cochin, Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Lacombe","sequence":"additional","affiliation":[{"name":"From the Department of Hematology, AP-HP, Ho\u0302pital Cochin; INSERM U363, Institut Cochin de Ge\u0301ne\u0301tique Mole\u0301culaire, Universite\u0301 Rene\u0301-Descartes, Paris V; and the Laboratory of Histology Embryology and Cytogenetics, AP-HP, Ho\u0302pital Cochin, Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franc\u0327ois","family":"Dreyfus","sequence":"additional","affiliation":[{"name":"From the Department of Hematology, AP-HP, Ho\u0302pital Cochin; INSERM U363, Institut Cochin de Ge\u0301ne\u0301tique Mole\u0301culaire, Universite\u0301 Rene\u0301-Descartes, Paris V; and the Laboratory of Histology Embryology and Cytogenetics, AP-HP, Ho\u0302pital Cochin, Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Mayeux","sequence":"additional","affiliation":[{"name":"From the Department of Hematology, AP-HP, Ho\u0302pital Cochin; INSERM U363, Institut Cochin de Ge\u0301ne\u0301tique Mole\u0301culaire, Universite\u0301 Rene\u0301-Descartes, Paris V; and the Laboratory of Histology Embryology and Cytogenetics, AP-HP, Ho\u0302pital Cochin, Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michae\u0308la","family":"Fontenay-Roupie","sequence":"additional","affiliation":[{"name":"From the Department of Hematology, AP-HP, Ho\u0302pital Cochin; INSERM U363, Institut Cochin de Ge\u0301ne\u0301tique Mole\u0301culaire, Universite\u0301 Rene\u0301-Descartes, Paris V; and the Laboratory of Histology Embryology and Cytogenetics, AP-HP, Ho\u0302pital Cochin, Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"234","reference":[{"key":"2020021220330678500_B1","first-page":"771","article-title":"In vitro studies of erythropoietin dependent regulation of erythropoiesis in myelodysplastic syndromes.","volume":"4","author":"Merchav, Nielsen","year":"1990","journal-title":"Leukemia."},{"key":"2020021220330678500_B2","first-page":"340","article-title":"Impaired response of myelodysplastic marrow progenitors to stimulation with recombinant haemopoietic growth 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