{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T06:25:36Z","timestamp":1759991136410},"reference-count":36,"publisher":"American Society of Hematology","issue":"6","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2002,3,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We have studied the impact of B-cell receptor (BCR) or CD40 ligation on the in vitro chemotactic response of tonsillar B cells to 4 chemokines: stromal cell\u2013derived factor (SDF)\u20131\u03b1, macrophage inflammatory protein (MIP)\u20133\u03b1, MIP-3\u03b2, and B-cell\u2013attracting chemokine (BCA)\u20131. In the tonsil, SDF-1 and MIP-3\u03b1 are both expressed in the crypt epithelium, while MIP-3\u03b2 is found in the T zone and BCA-1 in the follicles. Resting virgin and memory B cells display a similar chemotaxis pattern, and they both have the potential to migrate in vitro to all 4 chemokines studied. This pattern of responsiveness is strongly modified by a surrogate antigen (Ag) but is not altered by CD40 ligand. We report here that surrogate Ag induces a profound and sustained suppression of the response to the crypt chemokines SDF-1\u03b1 and MIP-3\u03b1, while it exacerbates the migratory response to MIP-3\u03b2. The effect of surrogate Ag on the response to BCA-1 is biphasic: After an initial phase of suppression, chemotaxis toward BCA-1 is strongly up-regulated. Our results suggest that Ag is primarily responsible for reprogramming the B-cell chemotaxis responsiveness during the humoral response. We propose that it initiates an ordered change of the chemotaxis machinery allowing Ag-activated B cells to relocate in the T zone and B-cell follicles sequentially.<\/jats:p>","DOI":"10.1182\/blood.v99.6.1913","type":"journal-article","created":{"date-parts":[[2002,10,1]],"date-time":"2002-10-01T21:48:18Z","timestamp":1033508898000},"page":"1913-1921","update-policy":"http:\/\/dx.doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":30,"title":["BCR ligation reprograms B cells for migration to the T zone and B-cell follicle sequentially"],"prefix":"10.1182","volume":"99","author":[{"given":"Montserrat","family":"Casamayor-Palleja\u0300","sequence":"first","affiliation":[{"name":"From INSERM U404, Immunite\u0301 et Vaccination, and INSERM U503 (C.V.), Lyon, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul","family":"Mondie\u0300re","sequence":"additional","affiliation":[{"name":"From INSERM U404, Immunite\u0301 et Vaccination, and INSERM U503 (C.V.), Lyon, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claire","family":"Verschelde","sequence":"additional","affiliation":[{"name":"From INSERM U404, Immunite\u0301 et Vaccination, and INSERM U503 (C.V.), Lyon, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chantal","family":"Bella","sequence":"additional","affiliation":[{"name":"From INSERM U404, Immunite\u0301 et Vaccination, and INSERM U503 (C.V.), Lyon, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thierry","family":"Defrance","sequence":"additional","affiliation":[{"name":"From INSERM U404, Immunite\u0301 et Vaccination, and INSERM U503 (C.V.), Lyon, France."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"234","reference":[{"key":"2020021221574595300_B1","doi-asserted-by":"crossref","first-page":"2098","DOI":"10.1126\/science.286.5447.2098","article-title":"Chemokines and cell migration in secondary lymphoid 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