{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T22:43:15Z","timestamp":1774564995627,"version":"3.50.1"},"reference-count":24,"publisher":"American Society of Hematology","issue":"7","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2002,4,1]]},"abstract":"<jats:p>Leukocyte traffic into lymph nodes and sites of inflammation is guided by L-selectin. Experiments performed in vitro and with gene-deleted mice suggest that CD34 recognizes L-selectin if decorated by 6-sulfo sialyl Lewis x (sLex) saccharides and the MECA-79 epitope. However, very little is known about glycosylation of human L-selectin ligands. We report here on matrix-assisted laser desorption\/ionization time-of-flight mass spectrometry (MALDI-TOF MS) profiles of N- and O-linked oligosaccharide fractions from human tonsillar endothelial CD34. All detected O-glycans were sialylated; some were also monosulfated or monosulfated and monofucosylated. If a given CD34-glycan may carry all requirements for L-selectin recognition, that is, both 6-sulfo-sLex and MECA-79 epitopes, only one O-glycan fraction, O-9, SA2Hex3HexNAc3- Fuc1(SO3)1, meets the criteria. A candidate structure is SA\u03b12-3Gal\u03b21-4(Fuc\u03b11-3)(6-sulfo)GlcNAc\u03b21-3Gal\u03b21-3(SA\u03b12-3Gal\u03b21-4GlcNAc\u03b21-6)GalNAc. However, if sulfo sLex glycans are supplemented with separate sulfated, nonfucosylated O-glycans, saccharides in O-6, O-8, or O-9, putatively carrying MECA-79 epitopes, could form multiglycan binding epitopes for L-selectin.<\/jats:p>","DOI":"10.1182\/blood.v99.7.2609","type":"journal-article","created":{"date-parts":[[2002,10,11]],"date-time":"2002-10-11T18:33:52Z","timestamp":1034361232000},"page":"2609-2611","update-policy":"https:\/\/doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":35,"title":["O-glycans on human high endothelial CD34 putatively participating in L-selectin recognition"],"prefix":"10.1182","volume":"99","author":[{"given":"Tero","family":"Satomaa","sequence":"first","affiliation":[{"name":"From the Institute of Biotechnology and Department of Biosciences; the Biomedicum and Haartman Institute, Rational Drug Design Program, Department of Bacteriology and Immunology, University of Helsinki, Finland; and the Helsinki University Central Hospital Laboratory Diagnostics and Department of Otorhinolaryngology; Helsinki, Finland."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ossi","family":"Renkonen","sequence":"additional","affiliation":[{"name":"From the Institute of Biotechnology and Department of Biosciences; the Biomedicum and Haartman Institute, Rational Drug Design Program, Department of Bacteriology and Immunology, University of Helsinki, Finland; and the Helsinki University Central Hospital Laboratory Diagnostics and Department of Otorhinolaryngology; Helsinki, Finland."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jari","family":"Helin","sequence":"additional","affiliation":[{"name":"From the Institute of Biotechnology and Department of Biosciences; the Biomedicum and Haartman Institute, Rational Drug Design Program, Department of Bacteriology and Immunology, University of Helsinki, Finland; and the Helsinki University Central Hospital Laboratory Diagnostics and Department of Otorhinolaryngology; Helsinki, Finland."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juha","family":"Kirveskari","sequence":"additional","affiliation":[{"name":"From the Institute of Biotechnology and Department of Biosciences; the Biomedicum and Haartman Institute, Rational Drug Design Program, Department of Bacteriology and Immunology, University of Helsinki, Finland; and the Helsinki University Central Hospital Laboratory Diagnostics and Department of Otorhinolaryngology; Helsinki, Finland."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antti","family":"Ma\u0308kitie","sequence":"additional","affiliation":[{"name":"From the Institute of Biotechnology and Department of Biosciences; the Biomedicum and Haartman Institute, Rational Drug Design Program, Department of Bacteriology and Immunology, University of Helsinki, Finland; and the Helsinki University Central Hospital Laboratory Diagnostics and Department of Otorhinolaryngology; Helsinki, Finland."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Risto","family":"Renkonen","sequence":"additional","affiliation":[{"name":"From the Institute of Biotechnology and Department of Biosciences; the Biomedicum and Haartman Institute, Rational Drug Design Program, Department of Bacteriology and Immunology, University of Helsinki, Finland; and the Helsinki University Central Hospital Laboratory Diagnostics and Department of Otorhinolaryngology; Helsinki, Finland."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"234","reference":[{"key":"2020021223164197200_B1","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1126\/science.7692600","article-title":"Binding of L-selectin to the vascular sialomucin CD34.","volume":"262","author":"Baumheter","year":"1993","journal-title":"Science."},{"key":"2020021223164197200_B2","doi-asserted-by":"crossref","first-page":"1013","DOI":"10.1016\/S0002-9440(10)65201-7","article-title":"Endothelial ligands for 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