{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T07:42:58Z","timestamp":1777621378445,"version":"3.51.4"},"reference-count":46,"publisher":"American Society of Hematology","issue":"18","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2018,9,25]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Chronic graft-versus-host disease (cGVHD) causes significant morbidity and mortality in patients after allogeneic bone marrow (BM) or stem cell transplantation (allo-SCT). Recent work has indicated that both T and B lymphocytes play an important role in the pathophysiology of cGVHD. Previously, our group showed a critical role for the germinal center response in the function of B cells using a bronchiolitis obliterans (BO) model of cGVHD. Here, we demonstrated for the first time that cGVHD is associated with severe defects in the generation of BM B lymphoid and uncommitted common lymphoid progenitor cells. We found an increase in the number of donor CD4+ T cells in the BM of mice with cGVHD that was negatively correlated with B-cell development and the frequency of osteoblasts and Prrx-1\u2013expressing perivascular stromal cells, which are present in the B-cell niche. Use of anti-DR3 monoclonal antibodies to enhance the number of donor regulatory T cells (Tregs) in the donor T-cell inoculum ameliorated the pathology associated with BO in this model. This correlated with an increased number of endosteal osteoblastic cells and significantly improved the generation of B-cell precursors in the BM after allo-SCT. Our work indicates that donor Tregs play a critical role in preserving the generation of B-cell precursors in the BM after allo-SCT. Approaches to enhance the number and\/or function of donor Tregs that do not enhance conventional T-cell activity may be important to decrease the incidence and severity of cGVHD in part through normal B-cell lymphopoiesis.<\/jats:p>","DOI":"10.1182\/bloodadvances.2017014977","type":"journal-article","created":{"date-parts":[[2018,9,18]],"date-time":"2018-09-18T15:53:28Z","timestamp":1537286008000},"page":"2307-2319","update-policy":"https:\/\/doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":17,"title":["Impaired bone marrow B-cell development in mice with a bronchiolitis obliterans model of cGVHD"],"prefix":"10.1182","volume":"2","author":[{"given":"Oleg V.","family":"Kolupaev","sequence":"first","affiliation":[{"name":"UNC Lineberger Comprehensive Cancer Center,"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Trisha A.","family":"Dant","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hemamalini","family":"Bommiasamy","sequence":"additional","affiliation":[{"name":"UNC Lineberger Comprehensive Cancer Center,"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danny W.","family":"Bruce","sequence":"additional","affiliation":[{"name":"UNC Lineberger Comprehensive Cancer Center,"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenneth A.","family":"Fowler","sequence":"additional","affiliation":[{"name":"UNC Lineberger Comprehensive Cancer Center,"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen L.","family":"Tilley","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen P.","family":"McKinnon","sequence":"additional","affiliation":[{"name":"UNC Lineberger Comprehensive Cancer Center,"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefanie","family":"Sarantopoulos","sequence":"additional","affiliation":[{"name":"Division of Hematologic Malignancies and Cellular Therapy, Duke Cancer Institute, Duke University, Durham, N\u0421; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruce R.","family":"Blazar","sequence":"additional","affiliation":[{"name":"Masonic Cancer Center, Minneapolis, MN"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James M.","family":"Coghill","sequence":"additional","affiliation":[{"name":"UNC Lineberger Comprehensive Cancer Center,"},{"name":"Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonathan S.","family":"Serody","sequence":"additional","affiliation":[{"name":"UNC Lineberger Comprehensive Cancer Center,"},{"name":"Department of Microbiology and Immunology, and"},{"name":"Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC;"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"234","published-online":{"date-parts":[[2018,9,18]]},"reference":[{"issue":"1","key":"2020021108364576800_B1","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1182\/blood-2016-06-686618","article-title":"Chronic graft-versus-host disease: biological insights from preclinical and clinical studies","volume":"129","author":"MacDonald","year":"2017","journal-title":"Blood"},{"issue":"5","key":"2020021108364576800_B2","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1038\/nri2000","article-title":"Graft-versus-host disease","volume":"7","author":"Shlomchik","year":"2007","journal-title":"Nat Rev Immunol"},{"issue":"20","key":"2020021108364576800_B3","doi-asserted-by":"crossref","first-page":"4327","DOI":"10.1182\/blood-2009-06-204669","article-title":"Acute graft-versus-host disease: from the bench to the 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