{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T14:16:01Z","timestamp":1792678561990,"version":"4.5.0"},"reference-count":71,"publisher":"American Society of Hematology","issue":"20","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2018,10,23]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The limited number of hematopoietic stem cells (HSCs) in umbilical cord blood (UCB) units restricts their use for stem cell transplantation. Ex vivo treatment of UCB-CD34+ cells with valproic acid (VPA) increases the number of transplantable HSCs. In this study, we demonstrate that HSC expansion is not merely a result of proliferation of the existing stem cells but, rather, a result of a rapid reprogramming of CD34+CD90\u2212 cells into CD34+CD90+ cells, which is accompanied by limited numbers of cell divisions. Beyond this phenotypic switch, the treated cells acquire and retain a transcriptomic and mitochondrial profile, reminiscent of primary HSCs. Single and bulk RNA-seq revealed a signature highly enriched for transcripts characteristic of primary HSCs. The acquisition of this HSC signature is linked to mitochondrial remodeling accompanied by a reduced activity and enhanced glycolytic potential. These events act in concert with a modest upregulation of p53 activity to limit the levels of reactive oxygen species (ROS). Inhibition of either glycolysis or p53 activity impairs HSC expansion. This study indicates that a complex interplay of events is required for effective ex vivo expansion of UCB-HSCs.<\/jats:p>","DOI":"10.1182\/bloodadvances.2018024273","type":"journal-article","created":{"date-parts":[[2018,10,22]],"date-time":"2018-10-22T11:53:26Z","timestamp":1540209206000},"page":"2766-2779","update-policy":"https:\/\/doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":46,"title":["Ex vivo human HSC expansion requires coordination of cellular reprogramming with mitochondrial remodeling and p53 activation"],"prefix":"10.1182","volume":"2","author":[{"given":"Luena","family":"Papa","sequence":"first","affiliation":[{"name":"Division of Hematology\/Oncology, Tisch Cancer Institute,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eran","family":"Zimran","sequence":"additional","affiliation":[{"name":"Division of Hematology\/Oncology, Tisch Cancer Institute,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mansour","family":"Djedaini","sequence":"additional","affiliation":[{"name":"Division of Hematology\/Oncology, Tisch Cancer Institute,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongchao","family":"Ge","sequence":"additional","affiliation":[{"name":"Department of Neurology, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Umut","family":"Ozbek","sequence":"additional","affiliation":[{"name":"Division of Hematology\/Oncology, Tisch Cancer Institute,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Sebra","sequence":"additional","affiliation":[{"name":"Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, NY"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5791-1217","authenticated-orcid":false,"given":"Stuart C.","family":"Sealfon","sequence":"additional","affiliation":[{"name":"Department of Neurology, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronald","family":"Hoffman","sequence":"additional","affiliation":[{"name":"Division of Hematology\/Oncology, Tisch Cancer Institute,"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"234","published-online":{"date-parts":[[2018,10,22]]},"reference":[{"issue":"6","key":"2020021108384565800_B1","doi-asserted-by":"crossref","first-page":"2378","DOI":"10.1172\/JCI70313","article-title":"Epigenetic reprogramming induces the expansion of cord blood stem cells","volume":"124","author":"Chaurasia","year":"2014","journal-title":"J Clin Invest"},{"issue":"4","key":"2020021108384565800_B2","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1097\/MOH.0000000000000143","article-title":"Role of epigenetic reprogramming in hematopoietic stem cell function","volume":"22","author":"Iancu-Rubin","year":"2015","journal-title":"Curr Opin Hematol"},{"issue":"3","key":"2020021108384565800_B3","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1016\/j.stem.2010.07.011","article-title":"The distinct metabolic profile of hematopoietic stem cells reflects their location in a hypoxic niche","volume":"7","author":"Simsek","year":"2010","journal-title":"Cell Stem Cell"},{"issue":"3","key":"2020021108384565800_B4","first-page":"222","article-title":"The study of gap junctional intercellular communication in keratinocytes as screening of promoter effect induced by industrial and environmental toxic substances","volume":"96","author":"Zefferino","year":"2005","journal-title":"Med Lav"},{"issue":"4","key":"2020021108384565800_B5","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1097\/MOH.0b013e328360ab4d","article-title":"Metabolic plasticity and hematopoietic stem cell biology","volume":"20","author":"Hsu","year":"2013","journal-title":"Curr Opin Hematol"},{"issue":"1","key":"2020021108384565800_B6","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.stem.2012.10.011","article-title":"Regulation of glycolysis by Pdk functions as a metabolic checkpoint for cell cycle quiescence in hematopoietic stem cells","volume":"12","author":"Takubo","year":"2013","journal-title":"Cell Stem Cell"},{"issue":"8","key":"2020021108384565800_B7","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1016\/j.tcb.2014.04.001","article-title":"Surviving change: the metabolic journey of hematopoietic stem cells","volume":"24","author":"Kohli","year":"2014","journal-title":"Trends Cell Biol"},{"issue":"6039","key":"2020021108384565800_B8","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1126\/science.1201219","article-title":"Isolation of single human hematopoietic stem cells capable of long-term multilineage engraftment","volume":"333","author":"Notta","year":"2011","journal-title":"Science"},{"issue":"1","key":"2020021108384565800_B9","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.stem.2012.11.022","article-title":"Metabolic regulation by the mitochondrial phosphatase PTPMT1 is required for hematopoietic stem cell differentiation","volume":"12","author":"Yu","year":"2013","journal-title":"Cell Stem Cell"},{"issue":"6","key":"2020021108384565800_B10","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1038\/ncb3529","article-title":"The mitochondrial respiratory chain is essential for haematopoietic stem cell function","volume":"19","author":"Ans\u00f3","year":"2017","journal-title":"Nat Cell Biol"},{"key":"2020021108384565800_B11","doi-asserted-by":"crossref","first-page":"13125","DOI":"10.1038\/ncomms13125","article-title":"Specification of haematopoietic stem cell fate via modulation of mitochondrial activity","volume":"7","author":"Vannini","year":"2016","journal-title":"Nat Commun"},{"issue":"8","key":"2020021108384565800_B12","doi-asserted-by":"crossref","first-page":"3056","DOI":"10.1182\/blood-2007-05-087759","article-title":"A low level of reactive oxygen species selects for primitive hematopoietic stem cells that may reside in the low-oxygenic niche","volume":"110","author":"Jang","year":"2007","journal-title":"Blood"},{"issue":"4","key":"2020021108384565800_B13","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1038\/nm1388","article-title":"Reactive oxygen species act through p38 MAPK to limit the lifespan of hematopoietic stem cells [published correction appears in Nat Med. 2010;16(1):129]","volume":"12","author":"Ito","year":"2006","journal-title":"Nat Med"},{"issue":"7011","key":"2020021108384565800_B14","doi-asserted-by":"crossref","first-page":"997","DOI":"10.1038\/nature02989","article-title":"Regulation of oxidative stress by ATM is required for self-renewal of haematopoietic stem cells","volume":"431","author":"Ito","year":"2004","journal-title":"Nature"},{"issue":"2","key":"2020021108384565800_B15","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/j.cell.2007.01.003","article-title":"FoxOs are critical mediators of hematopoietic stem cell resistance to physiologic oxidative stress","volume":"128","author":"Tothova","year":"2007","journal-title":"Cell"},{"issue":"13","key":"2020021108384565800_B16","doi-asserted-by":"crossref","first-page":"5431","DOI":"10.1073\/pnas.0701152104","article-title":"Distribution of hematopoietic stem cells in the bone marrow according to regional hypoxia","volume":"104","author":"Parmar","year":"2007","journal-title":"Proc Natl Acad Sci USA"},{"issue":"7263","key":"2020021108384565800_B17","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1038\/nature08313","article-title":"Reactive oxygen species prime Drosophila haematopoietic progenitors for differentiation","volume":"461","author":"Owusu-Ansah","year":"2009","journal-title":"Nature"},{"issue":"4","key":"2020021108384565800_B18","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1016\/j.stem.2011.09.010","article-title":"Metabolic regulation of hematopoietic stem cells in the hypoxic niche","volume":"9","author":"Suda","year":"2011","journal-title":"Cell Stem Cell"},{"issue":"10","key":"2020021108384565800_B19","doi-asserted-by":"crossref","first-page":"2397","DOI":"10.1084\/jem.20081297","article-title":"TSC-mTOR maintains quiescence and function of hematopoietic stem cells by repressing mitochondrial biogenesis and reactive oxygen species","volume":"205","author":"Chen","year":"2008","journal-title":"J Exp Med"},{"issue":"10","key":"2020021108384565800_B20","doi-asserted-by":"crossref","first-page":"2008","DOI":"10.4161\/cc.9.10.11733","article-title":"Upregulation of nascent mitochondrial biogenesis in mouse hematopoietic stem cells parallels upregulation of CD34 and loss of pluripotency: a potential strategy for reducing oxidative risk in stem cells","volume":"9","author":"Mantel","year":"2010","journal-title":"Cell Cycle"},{"issue":"7","key":"2020021108384565800_B21","doi-asserted-by":"crossref","first-page":"993","DOI":"10.3324\/haematol.2013.084293","article-title":"Mitochondria underlie different metabolism of hematopoietic stem and progenitor cells","volume":"98","author":"Arranz","year":"2013","journal-title":"Haematologica"},{"issue":"1","key":"2020021108384565800_B22","doi-asserted-by":"crossref","first-page":"7901","DOI":"10.1038\/ncomms8901","article-title":"An MTCH2 pathway repressing mitochondria metabolism regulates haematopoietic stem cell fate","volume":"6","author":"Maryanovich","year":"2015","journal-title":"Nat Commun"},{"issue":"3","key":"2020021108384565800_B23","doi-asserted-by":"crossref","first-page":"e12756","DOI":"10.1111\/acel.12756","article-title":"The mitochondrial unfolded protein response is activated upon hematopoietic stem cell exit from quiescence","volume":"17","author":"Mohrin","year":"2018","journal-title":"Aging Cell"},{"issue":"7644","key":"2020021108384565800_B24","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1038\/nature21388","article-title":"Autophagy maintains the metabolism and function of young and old stem cells","volume":"543","author":"Ho","year":"2017","journal-title":"Nature"},{"issue":"4","key":"2020021108384565800_B25","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1002\/stem.404","article-title":"The senescence-related mitochondrial\/oxidative stress pathway is repressed in human induced pluripotent stem cells","volume":"28","author":"Prigione","year":"2010","journal-title":"Stem Cells"},{"issue":"2","key":"2020021108384565800_B26","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1016\/j.cmet.2011.06.011","article-title":"Somatic oxidative bioenergetics transitions into pluripotency-dependent glycolysis to facilitate nuclear reprogramming","volume":"14","author":"Folmes","year":"2011","journal-title":"Cell Metab"},{"issue":"1","key":"2020021108384565800_B27","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1038\/cr.2011.177","article-title":"The metabolome of induced pluripotent stem cells reveals metabolic changes occurring in somatic cell reprogramming","volume":"22","author":"Panopoulos","year":"2012","journal-title":"Cell Res"},{"issue":"4","key":"2020021108384565800_B28","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1002\/stem.307","article-title":"Human induced pluripotent stem cell lines show stress defense mechanisms and mitochondrial regulation similar to those of human embryonic stem cells","volume":"28","author":"Armstrong","year":"2010","journal-title":"Stem Cells"},{"issue":"7259","key":"2020021108384565800_B29","doi-asserted-by":"crossref","first-page":"1140","DOI":"10.1038\/nature08311","article-title":"Linking the p53 tumour suppressor pathway to somatic cell reprogramming","volume":"460","author":"Kawamura","year":"2009","journal-title":"Nature"},{"issue":"7259","key":"2020021108384565800_B30","doi-asserted-by":"crossref","first-page":"1132","DOI":"10.1038\/nature08235","article-title":"Suppression of induced pluripotent stem cell generation by the p53-p21 pathway","volume":"460","author":"Hong","year":"2009","journal-title":"Nature"},{"issue":"6937","key":"2020021108384565800_B31","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1038\/nature01587","article-title":"Bmi-1 is required for maintenance of adult self-renewing haematopoietic stem cells","volume":"423","author":"Park","year":"2003","journal-title":"Nature"},{"issue":"6","key":"2020021108384565800_B32","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1016\/j.stem.2009.11.001","article-title":"Cited2 is an essential regulator of adult hematopoietic stem cells","volume":"5","author":"Kranc","year":"2009","journal-title":"Cell Stem Cell"},{"issue":"12","key":"2020021108384565800_B33","doi-asserted-by":"crossref","first-page":"1306","DOI":"10.1038\/nm1320","article-title":"The antioxidant function of the p53 tumor suppressor","volume":"11","author":"Sablina","year":"2005","journal-title":"Nat Med"},{"issue":"1","key":"2020021108384565800_B34","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.cmet.2013.06.002","article-title":"TXNIP maintains the hematopoietic cell pool by switching the function of p53 under oxidative stress","volume":"18","author":"Jung","year":"2013","journal-title":"Cell Metab"},{"issue":"10","key":"2020021108384565800_B35","doi-asserted-by":"crossref","first-page":"1236","DOI":"10.1016\/j.exphem.2008.04.012","article-title":"Enrichment of hematopoietic stem cells with SLAM and LSK markers for the detection of hematopoietic stem cell function in normal and Trp53 null mice","volume":"36","author":"Chen","year":"2008","journal-title":"Exp Hematol"},{"issue":"26","key":"2020021108384565800_B36","doi-asserted-by":"crossref","first-page":"5118","DOI":"10.1182\/blood-2012-05-356014","article-title":"The p53 pathway in hematopoiesis: lessons from mouse models, implications for humans","volume":"120","author":"Pant","year":"2012","journal-title":"Blood"},{"issue":"1","key":"2020021108384565800_B37","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.stem.2008.11.006","article-title":"p53 regulates hematopoietic stem cell quiescence","volume":"4","author":"Liu","year":"2009","journal-title":"Cell Stem Cell"},{"key":"2020021108384565800_B38","doi-asserted-by":"crossref","first-page":"903435","DOI":"10.1155\/2012\/903435","article-title":"Roles of p53 in various biological aspects of hematopoietic stem cells","volume":"2012","author":"Nii","year":"2012","journal-title":"J Biomed Biotechnol"},{"issue":"12","key":"2020021108384565800_B39","doi-asserted-by":"crossref","first-page":"1189","DOI":"10.1055\/s-2004-835850","article-title":"Phosphodiesterase and thymidine phosphorylase-inhibiting salirepin derivatives from Symplocos racemosa","volume":"70","author":"Abbasi","year":"2004","journal-title":"Planta Med"},{"key":"2020021108384565800_B40","doi-asserted-by":"crossref","first-page":"14049","DOI":"10.1038\/ncomms14049","article-title":"Massively parallel digital transcriptional profiling of single cells","volume":"8","author":"Zheng","year":"2017","journal-title":"Nat Commun"},{"issue":"41","key":"2020021108384565800_B41","doi-asserted-by":"crossref","first-page":"35977","DOI":"10.1074\/jbc.M111.266254","article-title":"Pluripotency-related, valproic acid (VPA)-induced genome-wide histone H3 lysine 9 (H3K9) acetylation patterns in embryonic stem cells","volume":"286","author":"Hezroni","year":"2011","journal-title":"J Biol Chem"},{"issue":"6","key":"2020021108384565800_B42","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1089\/scd.2008.0235","article-title":"Valproic acid increases CXCR4 expression in hematopoietic stem\/progenitor cells by chromatin remodeling","volume":"18","author":"Gul","year":"2009","journal-title":"Stem Cells Dev"},{"issue":"4","key":"2020021108384565800_B43","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1016\/j.bbmt.2013.12.562","article-title":"Differential effects of epigenetic modifiers on the expansion and maintenance of human cord blood stem\/progenitor cells","volume":"20","author":"Mahmud","year":"2014","journal-title":"Biol Blood Marrow Transplant"},{"issue":"12","key":"2020021108384565800_B44","doi-asserted-by":"crossref","first-page":"1891","DOI":"10.1089\/ars.2012.5019","article-title":"Hypoxia and metabolic properties of hematopoietic stem cells","volume":"20","author":"Zhang","year":"2014","journal-title":"Antioxid Redox Signal"},{"issue":"13","key":"2020021108384565800_B45","doi-asserted-by":"crossref","first-page":"2843","DOI":"10.1084\/jem.20130751","article-title":"Gata2 is required for HSC generation and survival","volume":"210","author":"de Pater","year":"2013","journal-title":"J Exp Med"},{"issue":"2","key":"2020021108384565800_B46","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1016\/j.stem.2007.07.017","article-title":"FoxO transcription factors and stem cell homeostasis: insights from the hematopoietic system","volume":"1","author":"Tothova","year":"2007","journal-title":"Cell Stem Cell"},{"issue":"5","key":"2020021108384565800_B47","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1016\/j.immuni.2010.11.014","article-title":"Hes1 is a critical but context-dependent mediator of canonical Notch signaling in lymphocyte development and transformation","volume":"33","author":"Wendorff","year":"2010","journal-title":"Immunity"},{"issue":"5","key":"2020021108384565800_B48","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1016\/j.stem.2008.03.004","article-title":"Pbx1 regulates self-renewal of long-term hematopoietic stem cells by maintaining their quiescence","volume":"2","author":"Ficara","year":"2008","journal-title":"Cell Stem Cell"},{"issue":"4","key":"2020021108384565800_B49","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1016\/j.exphem.2011.12.006","article-title":"Aldehyde dehydrogenases are regulators of hematopoietic stem cell numbers and B-cell development","volume":"40","author":"Gasparetto","year":"2012","journal-title":"Exp Hematol"},{"issue":"2","key":"2020021108384565800_B50","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.cell.2004.07.004","article-title":"Tie2\/angiopoietin-1 signaling regulates hematopoietic stem cell quiescence in the bone marrow niche","volume":"118","author":"Arai","year":"2004","journal-title":"Cell"},{"issue":"25","key":"2020021108384565800_B51","doi-asserted-by":"crossref","first-page":"3344","DOI":"10.1182\/blood-2016-11-750729","article-title":"EPCR expression marks UM171-expanded CD34+ cord blood stem cells","volume":"129","author":"Fares","year":"2017","journal-title":"Blood"},{"issue":"12","key":"2020021108384565800_B52","doi-asserted-by":"crossref","first-page":"2661","DOI":"10.1182\/blood-2008-06-161117","article-title":"High GATA-2 expression inhibits human hematopoietic stem and progenitor cell function by effects on cell cycle","volume":"113","author":"Tipping","year":"2009","journal-title":"Blood"},{"issue":"3","key":"2020021108384565800_B53","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1016\/j.stem.2015.01.017","article-title":"CDK6 levels regulate quiescence exit in human hematopoietic stem cells","volume":"16","author":"Laurenti","year":"2015","journal-title":"Cell Stem Cell"},{"issue":"6","key":"2020021108384565800_B54","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1038\/nrm3591","article-title":"Molecular regulation of stem cell quiescence","volume":"14","author":"Cheung","year":"2013","journal-title":"Nat Rev Mol Cell Biol"},{"issue":"414","key":"2020021108384565800_B55","doi-asserted-by":"crossref","first-page":"eaan1145","DOI":"10.1126\/scitranslmed.aan1145","article-title":"A distinct hematopoietic stem cell population for rapid multilineage engraftment in nonhuman primates","volume":"9","author":"Radtke","year":"2017","journal-title":"Sci Transl Med"},{"issue":"5593","key":"2020021108384565800_B56","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1126\/science.1073823","article-title":"A stem cell molecular signature","volume":"298","author":"Ivanova","year":"2002","journal-title":"Science"},{"issue":"6","key":"2020021108384565800_B57","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1016\/j.stem.2017.11.002","article-title":"Dye-independent methods reveal elevated mitochondrial mass in hematopoietic stem cells","volume":"21","author":"de Almeida","year":"2017","journal-title":"Cell Stem Cell"},{"issue":"4","key":"2020021108384565800_B58","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1016\/j.stem.2016.08.008","article-title":"Glycolytic metabolism plays a functional role in regulating human pluripotent stem cell state","volume":"19","author":"Gu","year":"2016","journal-title":"Cell Stem Cell"},{"issue":"32","key":"2020021108384565800_B59","doi-asserted-by":"crossref","first-page":"33568","DOI":"10.18632\/oncotarget.5598","article-title":"Effective impairment of myeloma cells and their progenitors by blockade of monocarboxylate transportation","volume":"6","author":"Hanson","year":"2015","journal-title":"Oncotarget"},{"issue":"3","key":"2020021108384565800_B60","doi-asserted-by":"crossref","first-page":"C385","DOI":"10.1152\/ajpcell.00485.2010","article-title":"Hypoxia. 2. Hypoxia regulates cellular metabolism","volume":"300","author":"Wheaton","year":"2011","journal-title":"Am J Physiol Cell Physiol"},{"issue":"7","key":"2020021108384565800_B61","doi-asserted-by":"crossref","first-page":"1553","DOI":"10.1016\/j.cell.2015.04.054","article-title":"Enhancing hematopoietic stem cell transplantation efficacy by mitigating oxygen shock","volume":"161","author":"Mantel","year":"2015","journal-title":"Cell"},{"issue":"4","key":"2020021108384565800_B62","first-page":"787","article-title":"Histone deacetylase inhibitors valproic acid and depsipeptide sensitize retinoblastoma cells to radiotherapy by increasing H2AX phosphorylation and p53 acetylation-phosphorylation","volume":"37","author":"Kawano","year":"2010","journal-title":"Int J Oncol"},{"issue":"7","key":"2020021108384565800_B63","doi-asserted-by":"crossref","first-page":"2350","DOI":"10.1158\/0008-5472.CAN-2287-2","article-title":"p53-induced up-regulation of MnSOD and GPx but not catalase increases oxidative stress and apoptosis","volume":"64","author":"Hussain","year":"2004","journal-title":"Cancer Res"},{"issue":"10","key":"2020021108384565800_B64","doi-asserted-by":"crossref","first-page":"1852","DOI":"10.1002\/sctm.17-0048","article-title":"Brief report: a differential transcriptomic profile of ex vivo expanded adult human hematopoietic stem cells empowers them for engraftment better than their surface phenotype","volume":"6","author":"Psatha","year":"2017","journal-title":"Stem Cells Transl Med"},{"issue":"17","key":"2020021108384565800_B65","doi-asserted-by":"crossref","first-page":"1957","DOI":"10.1089\/scd.2015.0117","article-title":"Connecting mitochondria, metabolism, and stem cell fate","volume":"24","author":"Wanet","year":"2015","journal-title":"Stem Cells Dev"},{"issue":"7259","key":"2020021108384565800_B66","doi-asserted-by":"crossref","first-page":"1085","DOI":"10.1038\/4601085a","article-title":"Stem cells: The promises and perils of p53","volume":"460","author":"Krizhanovsky","year":"2009","journal-title":"Nature"},{"issue":"5","key":"2020021108384565800_B67","doi-asserted-by":"crossref","first-page":"910","DOI":"10.1038\/leu.2011.315","article-title":"Synergistic induction of p53 mediated apoptosis by valproic acid and nutlin-3 in acute myeloid leukemia","volume":"26","author":"McCormack","year":"2012","journal-title":"Leukemia"},{"issue":"6","key":"2020021108384565800_B68","doi-asserted-by":"crossref","first-page":"1669","DOI":"10.1089\/ars.2010.3644","article-title":"p53, oxidative stress, and aging","volume":"15","author":"Liu","year":"2011","journal-title":"Antioxid Redox Signal"},{"issue":"4","key":"2020021108384565800_B69","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1038\/nrm2147","article-title":"p53 in health and disease","volume":"8","author":"Vousden","year":"2007","journal-title":"Nat Rev Mol Cell Biol"},{"issue":"11","key":"2020021108384565800_B70","doi-asserted-by":"crossref","first-page":"1269","DOI":"10.1038\/nbt.1502","article-title":"Induction of pluripotent stem cells from primary human fibroblasts with only Oct4 and Sox2","volume":"26","author":"Huangfu","year":"2008","journal-title":"Nat Biotechnol"},{"issue":"4","key":"2020021108384565800_B71","doi-asserted-by":"crossref","first-page":"686","DOI":"10.1016\/j.bbagen.2016.01.009","article-title":"Mitochondrial resetting and metabolic reprogramming in induced pluripotent stem cells and mitochondrial disease modeling","volume":"1860","author":"Hsu","year":"2016","journal-title":"Biochim Biophys Acta"}],"container-title":["Blood Advances"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/ashpublications.org\/bloodadvances\/article-pdf\/2\/20\/2766\/1629658\/advances024273.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/ashpublications.org\/bloodadvances\/article-pdf\/2\/20\/2766\/1629658\/advances024273.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,2,11]],"date-time":"2020-02-11T09:12:10Z","timestamp":1581412330000},"score":1,"resource":{"primary":{"URL":"https:\/\/ashpublications.org\/bloodadvances\/article\/2\/20\/2766\/16125\/Ex-vivo-human-HSC-expansion-requires-coordination"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,10,22]]},"references-count":71,"journal-issue":{"issue":"20","published-print":{"date-parts":[[2018,10,23]]}},"URL":"https:\/\/doi.org\/10.1182\/bloodadvances.2018024273","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.735505506.793566437","asserted-by":"object"}]},"ISSN":["2473-9529","2473-9537"],"issn-type":[{"value":"2473-9529","type":"print"},{"value":"2473-9537","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,10,22]]}}}