{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T08:13:52Z","timestamp":1789892032820,"version":"4.0.1"},"reference-count":31,"publisher":"American Society of Hematology","issue":"16","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2020,8,25]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Transfusion-related acute lung injury (TRALI) remains a leading cause of transfusion-related deaths. In most cases, anti-leukocyte antibodies in the transfusion product trigger TRALI, but not all anti-leukocyte antibodies cause TRALI. It has been shown that the anti\u2013major histocompatibility complex (MHC) class I antibody 34-1-2S (anti\u2013H-2Kd) causes TRALI in BALB\/c mice (MHC class I haplotype H-2Kd), whereas SF1.1.10 (anti\u2013H-2Kd) does not. In C57BL\/6 mice (MHC class I haplotype H-2Kb), TRALI only occurs when anti-MHC class I antibody AF6-88.5.5.3 (anti\u2013H-2Kb) is administered together with a high dose of 34-1-2S. It remains unknown which specific antibody characteristics are responsible for eliciting TRALI. We therefore investigated several biological and structural features of 34-1-2S compared with other anti-MHC class I antibodies, which on their own do not cause TRALI: SF1.1.10 and AF6-88.5.5.3. No substantial differences were observed between the TRALI-causing 34-1-2S and the TRALI-resistant SF1.1.10 regarding binding affinity to H-2Kd. Regarding binding affinity to H-2Kb, only AF6-88.5.5.3 potently bound to H-2Kb, whereas 34-1-2S exhibited weak but significant cross-reactivity. Furthermore, the binding affinity to Fc\u03b3Rs as well as the Fc glycan composition seemed to be similar for all antibodies. Similar Fc glycosylation profiles were also observed for human TRALI-causing donor anti-HLA antibodies compared with human anti-HLA antibodies from control donors. 34-1-2S, however, displayed superior complement activation capacity, which was fully Fc dependent and not significantly dependent on Fc glycosylation. We conclude that TRALI induction is not correlated with Fab- and Fc-binding affinities for antigen and Fc\u03b3Rs, respectively, nor with the composition of Fc glycans; but increased Fc-mediated complement activation is correlated with TRALI induction.<\/jats:p>","DOI":"10.1182\/bloodadvances.2020002291","type":"journal-article","created":{"date-parts":[[2020,8,18]],"date-time":"2020-08-18T17:46:31Z","timestamp":1597772791000},"page":"3875-3885","update-policy":"https:\/\/doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":16,"title":["Biological and structural characterization of murine TRALI antibody reveals increased Fc-mediated complement activation"],"prefix":"10.1182","volume":"4","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2784-5236","authenticated-orcid":false,"given":"Eveline A. N.","family":"Zeeuw van der Laan","sequence":"first","affiliation":[{"name":"Department of Experimental Immunohematology, Sanquin Research, and Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam, Amsterdam, The Netherlands;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saskia","family":"van der Velden","sequence":"additional","affiliation":[{"name":"Department of Experimental Immunohematology, Sanquin Research, and Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam, Amsterdam, The Netherlands;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arthur E. H.","family":"Bentlage","sequence":"additional","affiliation":[{"name":"Department of Experimental Immunohematology, Sanquin Research, and Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam, Amsterdam, The Netherlands;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1985-5901","authenticated-orcid":false,"given":"Mads D.","family":"Larsen","sequence":"additional","affiliation":[{"name":"Department of Experimental Immunohematology, Sanquin Research, and Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam, Amsterdam, The Netherlands;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thijs L. J.","family":"van Osch","sequence":"additional","affiliation":[{"name":"Department of Experimental Immunohematology, Sanquin Research, and Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam, Amsterdam, The Netherlands;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juk Yee","family":"Mok","sequence":"additional","affiliation":[{"name":"Sanquin Reagents, Amsterdam, The Netherlands;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giso","family":"Brasser","sequence":"additional","affiliation":[{"name":"Sanquin Reagents, Amsterdam, The Netherlands;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dionne M.","family":"Geerdes","sequence":"additional","affiliation":[{"name":"Sanquin Reagents, Amsterdam, The Netherlands;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolien A. M.","family":"Koeleman","sequence":"additional","affiliation":[{"name":"Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, The Netherlands;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Nouta","sequence":"additional","affiliation":[{"name":"Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, The Netherlands;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1510-0077","authenticated-orcid":false,"given":"John W.","family":"Semple","sequence":"additional","affiliation":[{"name":"Division of Hematology and Transfusion Medicine, Lund University, Lund, Sweden; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leendert","family":"Porcelijn","sequence":"additional","affiliation":[{"name":"Department of Immunohematology Diagnostics, Sanquin Diagnostic Services, Amsterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wim J. E.","family":"van Esch","sequence":"additional","affiliation":[{"name":"Sanquin Reagents, Amsterdam, The Netherlands;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0814-4995","authenticated-orcid":false,"given":"Manfred","family":"Wuhrer","sequence":"additional","affiliation":[{"name":"Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, The Netherlands;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. 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