{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T13:15:38Z","timestamp":1790514938757,"version":"4.1.0"},"reference-count":25,"publisher":"American Society of Hematology","issue":"12","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2021,6,22]]},"abstract":"<jats:p>Crosstalk between coagulation and innate immunity contributes to the progression of many diseases, including infection and cardiovascular disease. Venous thromboembolism (VTE), including pulmonary embolism and deep vein thrombosis (DVT), is among the most common causes of cardiovascular death. Here, we show that inflammasome activation and subsequent pyroptosis play an important role in the development of venous thrombosis. Using a flow restriction\u2013induced mouse venous thrombosis model in the inferior vena cava (IVC), we show that deficiency of caspase-1, but not caspase-11, protected against flow restriction\u2013induced thrombosis. Interleukin-1\u03b2 expression increased in the IVC following ligation, indicating that inflammasome is activated during injury. Deficiency of gasdermin D (GSDMD), an essential mediator of pyroptosis, protected against restriction-induced venous thrombosis. After induction of venous thrombosis, fibrin was deposited in the veins of wild-type mice, as detected using immunoblotting with a monoclonal antibody that specifically recognizes mouse fibrin, but not in the caspase-1\u2013deficient or GSDMD-deficient mice. Depletion of macrophages by gadolinium chloride or deficiency of tissue factor also protected against venous thrombosis. Our data reveal that tissue factor released from pyroptotic monocytes and macrophages following inflammasome activation triggers thrombosis.<\/jats:p>","DOI":"10.1182\/bloodadvances.2020003041","type":"journal-article","created":{"date-parts":[[2021,6,21]],"date-time":"2021-06-21T15:46:02Z","timestamp":1624290362000},"page":"2619-2623","update-policy":"https:\/\/doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":64,"title":["Inflammasome activation promotes venous thrombosis through pyroptosis"],"prefix":"10.1182","volume":"5","author":[{"given":"Yan","family":"Zhang","sequence":"first","affiliation":[{"name":"Saha Cardiovascular Research Center, College of Medicine, University of Kentucky, Lexington, KY;"},{"name":"Department of Oncology, The First Affiliated Hospital of Soochow University, Suzhou, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Cui","sequence":"additional","affiliation":[{"name":"Department of Chemistry, College of Arts and Sciences, University of Kentucky, Lexington, KY;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoying","family":"Zhang","sequence":"additional","affiliation":[{"name":"Saha Cardiovascular Research Center, College of Medicine, University of Kentucky, Lexington, KY;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7725-7881","authenticated-orcid":false,"given":"Congqing","family":"Wu","sequence":"additional","affiliation":[{"name":"Saha Cardiovascular Research Center, College of Medicine, University of Kentucky, Lexington, KY;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8178-7102","authenticated-orcid":false,"given":"Ahmed","family":"Abdel-Latif","sequence":"additional","affiliation":[{"name":"Saha Cardiovascular Research Center, College of Medicine, University of Kentucky, Lexington, KY;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan S.","family":"Smyth","sequence":"additional","affiliation":[{"name":"Saha Cardiovascular Research Center, College of Medicine, University of Kentucky, Lexington, KY;"},{"name":"Veterans Affairs Medical Center, Lexington, KY;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshihiko","family":"Shiroishi","sequence":"additional","affiliation":[{"name":"RIKEN BioResource Research Center, Tsukuba, Ibaraki, Japan; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nigel","family":"Mackman","sequence":"additional","affiliation":[{"name":"Division of Hematology, Department of Medicine, UNC Blood Research Center, University of North Carolina at Chapel Hill, Chapel Hill, NC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yinan","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Chemistry, College of Arts and Sciences, University of Kentucky, Lexington, KY;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Tao","sequence":"additional","affiliation":[{"name":"Department of Oncology, The First Affiliated Hospital of Soochow University, Suzhou, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenyu","family":"Li","sequence":"additional","affiliation":[{"name":"Saha Cardiovascular Research Center, College of Medicine, University of Kentucky, Lexington, KY;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"234","published-online":{"date-parts":[[2021,6,21]]},"reference":[{"issue":"4","key":"2021062115415750500_B1","doi-asserted-by":"crossref","first-page":"e18","DOI":"10.1161\/CIR.0b013e3182009701","article-title":"American Heart Association Statistics Committee and Stroke Statistics Subcommittee. 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