{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T10:43:04Z","timestamp":1784889784471,"version":"3.55.0"},"reference-count":49,"publisher":"American Society of Hematology","issue":"5","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2021,3,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Circular RNAs (circRNAs) are dynamically regulated during differentiation and show cell type\u2013specific expression, which is altered in cancer and can have a direct impact on its various hallmarks. We hypothesized that circRNA expression is deregulated in acute myeloid leukemia (AML) and that circRNA candidates might contribute to the pathogenesis of the disease. To identify leukemia-associated and differentiation-independent changes in circRNA expression, we determined the circular RNAome of 61 AML patients and 16 healthy hematopoietic stem and progenitor cell (HSPC) samples using ribosomal RNA\u2013depleted RNA sequencing. We found hundreds of circRNAs that were differentially expressed between AML and healthy HSPCs. Gene set analysis found that many of these circRNAs were transcribed from genes implicated in leukemia biology. We discovered a circRNA derived from the T-cell transcription factor gene B cell CLL\/lymphoma 11B, circBCL11B, which was exclusively expressed in AML patients, but not detected in healthy HSPCs, and associated with a T-cell\u2013like gene expression signature. We were able to validate this finding in an independent cohort of 332 AML patients. Knockdown of circBCL11B had a negative effect on leukemic cell proliferation and resulted in increased cell death of leukemic cells, thereby suggesting circBCL11B as a novel functionally relevant candidate in AML pathogenesis. In summary, our study enables comprehensive insights into circRNA expression changes upon leukemic transformation and provides valuable information on the biology of leukemic cells and potential novel pathway dependencies that are relevant for AML therapy.<\/jats:p>","DOI":"10.1182\/bloodadvances.2020003230","type":"journal-article","created":{"date-parts":[[2021,3,8]],"date-time":"2021-03-08T16:36:26Z","timestamp":1615221386000},"page":"1490-1503","update-policy":"https:\/\/doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":29,"title":["Deregulated expression of circular RNAs in acute myeloid leukemia"],"prefix":"10.1182","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3826-5242","authenticated-orcid":false,"given":"Susanne","family":"Lux","sequence":"first","affiliation":[{"name":"Internal Medicine III, University Hospital Ulm, Ulm, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9859-1261","authenticated-orcid":false,"given":"Tamara J.","family":"Bl\u00e4tte","sequence":"additional","affiliation":[{"name":"Internal Medicine III, University Hospital Ulm, Ulm, Germany;"},{"name":"Department of Hematology, Oncology, and Tumorimmunology, Charit\u00e9 University Medicine, Berlin, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernhard","family":"Gillissen","sequence":"additional","affiliation":[{"name":"Department of Hematology, Oncology, and Tumorimmunology, Charit\u00e9 University Medicine, Berlin, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antje","family":"Richter","sequence":"additional","affiliation":[{"name":"Department of Hematology, Oncology, and Tumorimmunology, Charit\u00e9 University Medicine, Berlin, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sibylle","family":"Cocciardi","sequence":"additional","affiliation":[{"name":"Internal Medicine III, University Hospital Ulm, Ulm, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabrina","family":"Skambraks","sequence":"additional","affiliation":[{"name":"Internal Medicine III, University Hospital Ulm, Ulm, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaus","family":"Schwarz","sequence":"additional","affiliation":[{"name":"Institute for Transfusion Medicine, University of Ulm, Ulm, Germany; and"},{"name":"Institute for Clinical Transfusion Medicine and Immunogenetics Ulm, German Red Cross Blood Donor Service Baden-Wuerttemberg\u2013Hessen, Ulm, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hubert","family":"Schrezenmeier","sequence":"additional","affiliation":[{"name":"Institute for Transfusion Medicine, University of Ulm, Ulm, Germany; and"},{"name":"Institute for Clinical Transfusion Medicine and Immunogenetics Ulm, German Red Cross Blood Donor Service Baden-Wuerttemberg\u2013Hessen, Ulm, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hartmut","family":"D\u00f6hner","sequence":"additional","affiliation":[{"name":"Internal Medicine III, University Hospital Ulm, Ulm, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Konstanze","family":"D\u00f6hner","sequence":"additional","affiliation":[{"name":"Internal Medicine III, University Hospital Ulm, Ulm, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Dolnik","sequence":"additional","affiliation":[{"name":"Internal Medicine III, University Hospital Ulm, Ulm, Germany;"},{"name":"Department of Hematology, Oncology, and Tumorimmunology, Charit\u00e9 University Medicine, Berlin, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lars","family":"Bullinger","sequence":"additional","affiliation":[{"name":"Internal Medicine III, University Hospital Ulm, Ulm, Germany;"},{"name":"Department of Hematology, Oncology, and Tumorimmunology, Charit\u00e9 University Medicine, Berlin, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"234","published-online":{"date-parts":[[2021,3,8]]},"reference":[{"issue":"7441","key":"2021030816352629100_B1","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1038\/nature11928","article-title":"Circular RNAs are a large class of animal RNAs with regulatory potency","volume":"495","author":"Memczak","year":"2013","journal-title":"Nature"},{"issue":"7441","key":"2021030816352629100_B2","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1038\/nature11993","article-title":"Natural RNA circles function as efficient microRNA 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