{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T13:04:14Z","timestamp":1792760654724,"version":"4.5.0"},"reference-count":16,"publisher":"American Society of Hematology","issue":"6","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2023,3,28]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Immune thrombocytopenia (ITP) is an autoimmune bleeding disorder with an incompletely understood pathophysiology but includes platelet-clearance in the spleen and liver via T cells and\/or platelet autoantibodies. Strikingly, thrombopoietin (TPO) levels remain low in ITP. Platelet-glycoprotein (GP)Ib\u03b1 has been described to be required for hepatic TPO generation; however, the role of GPIb antibodies in relation to platelet hepatic sequestration and TPO levels, with consideration of platelet counts, remains to be elucidated. Therefore, we examined 53 patients with chronic and nonsplenectomized ITP for whom we conducted indium-labeled autologous platelet scintigraphy and measured platelet antibodies and TPO levels. Upon stratification toward the severity of thrombocytopenia, no negative association was observed between GPIb\/IX antibodies and TPO levels, suggesting that GPIb\/IX antibodies do not inhibit or block TPO levels. Surprisingly, we observed a positive association between GPIb\/IX antibody levels and TPO levels and GPIb\/IX antibodies and platelet hepatic sequestration in patients with severe, but not mild or moderate, thrombocytopenia. In addition, platelet hepatic sequestration and TPO levels were positively associated. This collectively indicates that GPIb\/IX antibodies may be associated with increased platelet hepatic sequestration and elevated TPO levels in patients with severe thrombocytopenic ITP; however, further research is warranted to elucidate the pathophysiologic mechanisms.<\/jats:p>","DOI":"10.1182\/bloodadvances.2022007751","type":"journal-article","created":{"date-parts":[[2022,7,28]],"date-time":"2022-07-28T19:26:17Z","timestamp":1659036377000},"page":"1066-1069","update-policy":"https:\/\/doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":16,"title":["The interplay between GPIb\/IX antibodies, platelet hepatic sequestration, and TPO levels in patients with chronic ITP"],"prefix":"10.1182","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9414-0044","authenticated-orcid":false,"given":"Sufia N.","family":"Amini","sequence":"first","affiliation":[{"name":"1Department of Hematology, Hagaziekenhuis, The Hague, The Netherlands"},{"name":"2Department of Hematology, Leiden University Medical Center, Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vivianne S.","family":"Nelson","sequence":"additional","affiliation":[{"name":"1Department of Hematology, Hagaziekenhuis, The Hague, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leendert","family":"Porcelijn","sequence":"additional","affiliation":[{"name":"3Sanquin Diagnostics, Amsterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tanja","family":"Netelenbos","sequence":"additional","affiliation":[{"name":"1Department of Hematology, Hagaziekenhuis, The Hague, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaap Jan","family":"Zwaginga","sequence":"additional","affiliation":[{"name":"2Department of Hematology, Leiden University Medical Center, Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masja","family":"de Haas","sequence":"additional","affiliation":[{"name":"2Department of Hematology, Leiden University Medical Center, Leiden, The Netherlands"},{"name":"3Sanquin Diagnostics, Amsterdam, The Netherlands"},{"name":"4Department of Clinical Transfusion Research, Sanquin Research, Amsterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin R.","family":"Schipperus","sequence":"additional","affiliation":[{"name":"5Department of Hematology, University Medical Center Groningen, Groningen, The Netherlands"},{"name":"6Sanquin Transfusion Services, Amsterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1608-876X","authenticated-orcid":false,"given":"Rick","family":"Kapur","sequence":"additional","affiliation":[{"name":"7Department of Experimental Immunohematology, Landsteiner Laboratory, Amsterdam University Medical Center, Sanquin Research, University of Amsterdam, Amsterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"234","published-online":{"date-parts":[[2023,3,22]]},"reference":[{"issue":"11","key":"2023081516035448800_bib1","doi-asserted-by":"crossref","first-page":"2386","DOI":"10.1182\/blood-2008-07-162503","article-title":"Standardization of terminology, definitions and outcome criteria in immune thrombocytopenic purpura of adults and children: report from an international working group","volume":"113","author":"Rodeghiero","year":"2009","journal-title":"Blood"},{"issue":"2","key":"2023081516035448800_bib2","first-page":"E16","article-title":"Pathogenesis and therapeutic mechanisms in immune thrombocytopenia (ITP)","volume":"6","author":"Zufferey","year":"2017","journal-title":"J\u00a0Clin Med"},{"issue":"5","key":"2023081516035448800_bib3","doi-asserted-by":"crossref","first-page":"623","DOI":"10.3324\/haematol.2014.115634","article-title":"Immune thrombocytopenia: antiplatelet autoantibodies inhibit proplatelet formation by megakaryocytes and impair platelet production in\u00a0vitro","volume":"100","author":"Iraqi","year":"2015","journal-title":"Haematologica"},{"issue":"4 suppl 5","key":"2023081516035448800_bib4","doi-asserted-by":"crossref","first-page":"S3","DOI":"10.1053\/j.seminhematol.2007.11.002","article-title":"The pathogenesis of chronic immune thrombocytopenic purpura","volume":"44","author":"McMillan","year":"2007","journal-title":"Semin Hematol"},{"issue":"4","key":"2023081516035448800_bib5","doi-asserted-by":"crossref","first-page":"e61","DOI":"10.3324\/haematol.2013.102897","article-title":"Severe platelet desialylation in a patient with glycoprotein Ib\/IX antibody-mediated immune thrombocytopenia and fatal pulmonary hemorrhage","volume":"99","author":"Li","year":"2014","journal-title":"Haematologica"},{"issue":"1","key":"2023081516035448800_bib6","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1080\/09537104.2019.1572877","article-title":"Plasma thrombopoietin levels as additional tool in clinical management of thrombocytopenic neonates","volume":"31","author":"Porcelijn","year":"2020","journal-title":"Platelets"},{"issue":"1","key":"2023081516035448800_bib7","doi-asserted-by":"crossref","first-page":"7737","DOI":"10.1038\/ncomms8737","article-title":"Desialylation is a mechanism of Fc-independent platelet clearance and a therapeutic target in immune thrombocytopenia","volume":"6","author":"Li","year":"2015","journal-title":"Nat Commun"},{"issue":"6","key":"2023081516035448800_bib8","doi-asserted-by":"crossref","first-page":"622","DOI":"10.1182\/blood-2017-12-820779","article-title":"GPIb\u03b1 is required for platelet-mediated hepatic thrombopoietin generation","volume":"132","author":"Xu","year":"2018","journal-title":"Blood"},{"issue":"4","key":"2023081516035448800_bib9","doi-asserted-by":"crossref","first-page":"e172","DOI":"10.3324\/haematol.2019.228908","article-title":"Anti-glycoprotein Ib\u03b1 autoantibodies do not impair circulating thrombopoietin levels in immune thrombocytopenia patients","volume":"105","author":"Porcelijn","year":"2020","journal-title":"Haematologica"},{"issue":"1","key":"2023081516035448800_bib10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1146\/annurev.med.48.1.1","article-title":"Thrombopoietin: understanding and manipulating platelet production","volume":"48","author":"Kaushansky","year":"1997","journal-title":"Annu Rev Med"},{"issue":"6","key":"2023081516035448800_bib11","doi-asserted-by":"crossref","first-page":"1797","DOI":"10.1182\/bloodadvances.2021004822","article-title":"Anti-glycoprotein antibodies and sequestration pattern of indium labeled platelets in immune thrombocytopenia","volume":"6","author":"Amini","year":"2021","journal-title":"Blood Adv"},{"issue":"3","key":"2023081516035448800_bib12","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1111\/bjh.15404","article-title":"Detection of platelet autoantibodies to identify immune thrombocytopenia: state of the art","volume":"182","author":"Porcelijn","year":"2018","journal-title":"Br J Haematol"},{"issue":"4","key":"2023081516035448800_bib13","doi-asserted-by":"crossref","first-page":"1262","DOI":"10.1055\/s-0038-1657725","article-title":"Sensitive measurement of thrombopoietin by a monoclonal antibody based sandwich enzymelinked immunosorbent assay","volume":"78","author":"Folman","year":"1997","journal-title":"Thromb Haemost"},{"issue":"5","key":"2023081516035448800_bib14","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1111\/j.1365-2141.2010.08377.x","article-title":"Autologous 111 In-labelled platelet sequestration studies in patients with primary immune thrombocytopenia (ITP) prior to splenectomy: a report from the United Kingdom ITP Registry","volume":"151","author":"Sarpatwari","year":"2010","journal-title":"Br J Haematol"},{"issue":"12","key":"2023081516035448800_bib15","doi-asserted-by":"crossref","first-page":"3131","DOI":"10.1111\/jth.15035","article-title":"Structure-function of platelet glycoprotein Ib-IX","volume":"18","author":"Quach","year":"2020","journal-title":"J\u00a0Thromb Haemost"},{"issue":"8","key":"2023081516035448800_bib16","doi-asserted-by":"crossref","first-page":"2044","DOI":"10.1111\/jth.15359","article-title":"Differential regulation of the platelet GPIb-IX complex by anti-GPIb\u03b2 antibodies","volume":"19","author":"Quach","year":"2021","journal-title":"J\u00a0Thromb Haemost"}],"container-title":["Blood Advances"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/ashpublications.org\/bloodadvances\/article-pdf\/7\/6\/1066\/2071529\/blooda_adv-2022-007751-main.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/ashpublications.org\/bloodadvances\/article-pdf\/7\/6\/1066\/2071529\/blooda_adv-2022-007751-main.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,15]],"date-time":"2023-08-15T16:15:51Z","timestamp":1692116151000},"score":1,"resource":{"primary":{"URL":"https:\/\/ashpublications.org\/bloodadvances\/article\/7\/6\/1066\/486055\/The-interplay-between-GPIb-IX-antibodies-platelet"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,22]]},"references-count":16,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2023,3,28]]}},"URL":"https:\/\/doi.org\/10.1182\/bloodadvances.2022007751","relation":{},"ISSN":["2473-9529","2473-9537"],"issn-type":[{"value":"2473-9529","type":"print"},{"value":"2473-9537","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3,22]]}}}