{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T03:05:40Z","timestamp":1780369540895,"version":"3.54.1"},"reference-count":49,"publisher":"American Society of Hematology","issue":"17","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2022,9,13]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>It is unknown how lower-leg injury and knee arthroscopy, both associated with venous thromboembolism (VTE), affect coagulation. To study the effect of (1) lower-leg trauma and (2) knee arthroscopy on coagulation, plasma samples of the Prevention of Thrombosis following CAST immobilization (POT-CAST, #NCT01542762) and Prevention of Thrombosis following Knee Arthroscopy (POT-KAST, #NCT01542723) trials were used, which were collected shortly after lower-leg trauma and before\/after\u00a0(&amp;lt;4 hours) knee arthroscopy. For aim 1, 1204 lower-leg injury patients were compared\u00a0with preoperative samples of 1001 controls. Mean differences\/ratios (if ln-retransformed because of skewedness) were adjusted for sex, age, body mass index, comorbidity, malignancy, and oral contraceptives using linear regression. For aim 2, perioperative mean changes of 715 arthroscopy patients were calculated. Plasma levels of fibrinogen, factor (F)VIII, FIX, FXI, von Willebrand Factor (VWF), and D-dimer were measured in all individuals. Parameters of underlying mechanisms (tissue factor, interleukin-6 [IL-6], myeloperoxidase DNA, cell-free DNA) were measured in random subsets. In lower-leg injury patients, coagulation parameter levels increased, especially FVIII, VWF, and D-dimer, that is, adjusted mean differences: FVIII 26.8% (95% confidence interval [CI], 23.7-29.9), FIX 13.8% (95% CI, 11.9-15.6), FXI 5.1% (95% CI, 3.3-7.0), VWF 29.8% (95% CI, 26.0-33.6), fibrinogen 32.5\u00a0mg\/dL (95% CI, 25.8-39.2), and D-dimer (mean ratio) 3.3 (95% CI, 3.1-3.6). Remaining parameters were unchanged, except for increased IL-6 levels. After arthroscopy, all parameters decreased. Lower-leg trauma is associated with increased procoagulant factor levels in contrast to knee arthroscopy. This suggests that, in both situations, different pathways are involved in development of VTE.<\/jats:p>","DOI":"10.1182\/bloodadvances.2022007828","type":"journal-article","created":{"date-parts":[[2022,5,24]],"date-time":"2022-05-24T21:15:20Z","timestamp":1653426920000},"page":"5232-5243","update-policy":"https:\/\/doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":7,"title":["Lower-leg injury and knee arthroscopy have distinct effects on coagulation"],"prefix":"10.1182","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7993-5209","authenticated-orcid":false,"given":"Carolina E.","family":"Touw","sequence":"first","affiliation":[{"name":"1Department of Clinical Epidemiology,"},{"name":"2Department of Orthopaedics, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Banne","family":"Nemeth","sequence":"additional","affiliation":[{"name":"1Department of Clinical Epidemiology,"},{"name":"2Department of Orthopaedics, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2703-8805","authenticated-orcid":false,"given":"Araci M. R.","family":"Rondon","sequence":"additional","affiliation":[{"name":"3Division of Thrombosis and Haemostasis, Department of Internal Medicine, Leiden University Medical Center, Leiden, The Netherlands;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raymond A.","family":"van Adrichem","sequence":"additional","affiliation":[{"name":"1Department of Clinical Epidemiology,"},{"name":"2Department of Orthopaedics, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3503-7140","authenticated-orcid":false,"given":"Ton","family":"Lisman","sequence":"additional","affiliation":[{"name":"4Surgical Research Laboratory, Department of Surgery, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henri H.","family":"Versteeg","sequence":"additional","affiliation":[{"name":"3Division of Thrombosis and Haemostasis, Department of Internal Medicine, Leiden University Medical Center, Leiden, The Netherlands;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9952-3079","authenticated-orcid":false,"given":"Inger B.","family":"Schipper","sequence":"additional","affiliation":[{"name":"5Department of Trauma Surgery, Leiden University Medical Center, Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rob G. 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