{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T22:40:38Z","timestamp":1771627238520,"version":"3.50.1"},"reference-count":0,"publisher":"American Society of Hematology","issue":"4","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1977,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The presence of human fibrinogen in suspensions of washed human platelets is a requirement for ADP-induced platelet aggregation. Digestion of fibrinogen with plasmin destroys this function of the protein. The high solubility fraction of Kabi fibrinogen, fragment X (stage 1) and framgent X (stage 2), are two, eight, and ten times, respectively, less potent in promoting ADP-induced platelet aggregation, as compared with intact fibrinogen. Fragments Y and D and the mixture of reduced and carboxymethylated chains of human fibrinogen do not support ADP-induced platelet aggregation at all. SDS polyacrylamide gel electrophoresis of nonreduced and reduced fibrinogen and its derivatives indicates that the intact fibrinogen molecule is essential for ADP-induced platelet aggregation. It is suggested that the carboxy-terminal part of the Aalpha chain and possibly also the amino-terminal part of the Bbeta chain are required for the platelet aggregation-promoting function of fibrinogen.<\/jats:p>","DOI":"10.1182\/blood.v49.4.635.635","type":"journal-article","created":{"date-parts":[[2019,10,13]],"date-time":"2019-10-13T00:16:37Z","timestamp":1570925797000},"page":"635-644","update-policy":"https:\/\/doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":54,"title":["Significance of the intact polypeptide chains of human fibrinogen in ADP-induced platelet aggregation"],"prefix":"10.1182","volume":"49","author":[{"given":"S","family":"Niewiarowski","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"AZ","family":"Budzynski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B","family":"Lipinski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"234","container-title":["Blood"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/ashpublications.org\/blood\/article-pdf\/49\/4\/635\/580172\/635.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/ashpublications.org\/blood\/article-pdf\/49\/4\/635\/580172\/635.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,11,19]],"date-time":"2019-11-19T14:51:28Z","timestamp":1574175088000},"score":1,"resource":{"primary":{"URL":"https:\/\/ashpublications.org\/blood\/article\/49\/4\/635\/160656\/Significance-of-the-intact-polypeptide-chains-of"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1977,4,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1977,4,1]]}},"URL":"https:\/\/doi.org\/10.1182\/blood.v49.4.635.635","relation":{},"ISSN":["0006-4971","1528-0020"],"issn-type":[{"value":"0006-4971","type":"print"},{"value":"1528-0020","type":"electronic"}],"subject":[],"published":{"date-parts":[[1977,4,1]]}}}