{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,25]],"date-time":"2025-11-25T13:49:15Z","timestamp":1764078555552},"reference-count":37,"publisher":"American Society of Hematology","issue":"11","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2007,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Monoclonal TCR\u03b1\u03b2+\/CD4+ T-large granular lymphocyte (T-LGL) lymphocytosis is a T-cell disorder with a restricted TCR-V\u03b2 repertoire. In the present study we explored the potential association between the expanded TCR-V\u03b2 families, the CDR3 sequences of the TCR-V\u03b2 gene, and the HLA genotype of patients with monoclonal TCR\u03b1\u03b2+\/CD4+ T-LGL lymphocytosis. For that purpose, 36 patients with monoclonal TCR\u03b1\u03b2+\/CD4+ T-LGL lymphocytosis (15 TCR-V\u03b213.1 versus 21 non\u2013TCR-V\u03b213.1) were selected. For each patient, both the HLA (class I and II) genotype and the DNA sequences of the VDJ-rearranged TCR-V\u03b2 were analyzed. Our results show a clear association between the TCR-V\u03b2 repertoire and the HLA genotype, all TCR-V\u03b213.1+ cases being HLA-DRB1*0701 (P = .004). Interestingly, the HLA-DR7\/TCR-V\u03b213.1\u2013restricted T-cell expansions displayed a highly homogeneous and strikingly similar TCR arising from the use of common TCR-V\u03b2 gene segments, which shared (1) unique CDR3 structural features with a constantly short length, (2) similar combinatorial gene rearrangements with frequent usage of the J\u03b21.1 gene, and (3) a homolog consensus protein sequence at recombination junctions. Overall, these findings strongly support the existence of a common antigen-driven origin for monoclonal CD4+ T-LGL lymphocytosis, with the identification of the exact peptides presented to the expanded T cells deserving further investigations.<\/jats:p>","DOI":"10.1182\/blood-2006-05-022277","type":"journal-article","created":{"date-parts":[[2007,2,15]],"date-time":"2007-02-15T20:47:06Z","timestamp":1171572426000},"page":"4890-4898","update-policy":"http:\/\/dx.doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":57,"title":["Monoclonal TCR-V\u03b213.1+\/CD4+\/NKa+\/CD8\u2212\/+dim T-LGL lymphocytosis: evidence for an antigen-driven chronic T-cell stimulation origin"],"prefix":"10.1182","volume":"109","author":[{"given":"Pilar","family":"Garrido","sequence":"first","affiliation":[{"name":"Servicio de Hematolog\u00eda, Hospital Universitario Virgen de las Nieves, Granada, Spain;"}]},{"given":"Francisco","family":"Ruiz-Cabello","sequence":"additional","affiliation":[{"name":"Servicio de An\u00e1lisis Cl\u00ednicos, Hospital Universitario Virgen de las Nieves, Granada, Spain;"}]},{"given":"Paloma","family":"B\u00e1rcena","sequence":"additional","affiliation":[{"name":"Servicio de Citometr\u00eda & Departamento de Medicina, Universidad de Salamanca (USAL), Spain;"},{"name":"Instituto de Biolog\u00eda Molecular y Celular del C\u00e1ncer, Centro de Investigaci\u00f3n del C\u00e1ncer\/IBMCC (CSIC-USAL), Salamanca, Spain;"}]},{"given":"Yorick","family":"Sandberg","sequence":"additional","affiliation":[{"name":"Department of Immunology, Erasmus Medical Center, Rotterdam, The Netherlands;"}]},{"given":"Julia","family":"Cant\u00f3n","sequence":"additional","affiliation":[{"name":"Servicio de Hematolog\u00eda, Hospital Universitario Virgen de las Nieves, Granada, Spain;"}]},{"given":"Margarida","family":"Lima","sequence":"additional","affiliation":[{"name":"Servicio de Hematolog\u00eda, Hospital Geral de Santo Ant\u00f3nio, Porto, Portugal;"}]},{"given":"Ana","family":"Balanzategui","sequence":"additional","affiliation":[{"name":"Instituto de Biolog\u00eda Molecular y Celular del C\u00e1ncer, Centro de Investigaci\u00f3n del C\u00e1ncer\/IBMCC (CSIC-USAL), Salamanca, Spain;"},{"name":"Servicio de Hematolog\u00eda, Hospital Universitario de Salamanca, Spain"}]},{"given":"Marcos","family":"Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Instituto de Biolog\u00eda Molecular y Celular del C\u00e1ncer, Centro de Investigaci\u00f3n del C\u00e1ncer\/IBMCC (CSIC-USAL), Salamanca, Spain;"},{"name":"Servicio de Hematolog\u00eda, Hospital Universitario de Salamanca, Spain"}]},{"given":"Miguel Angel","family":"L\u00f3pez-Nevot","sequence":"additional","affiliation":[{"name":"Servicio de Hematolog\u00eda, Hospital Universitario Virgen de las Nieves, Granada, Spain;"}]},{"given":"Anton W.","family":"Langerak","sequence":"additional","affiliation":[{"name":"Department of Immunology, Erasmus Medical Center, Rotterdam, The Netherlands;"}]},{"given":"Andr\u00e9s C.","family":"Garc\u00eda-Montero","sequence":"additional","affiliation":[{"name":"Servicio de Citometr\u00eda & Departamento de Medicina, Universidad de Salamanca (USAL), Spain;"},{"name":"Instituto de Biolog\u00eda Molecular y Celular del C\u00e1ncer, Centro de Investigaci\u00f3n del C\u00e1ncer\/IBMCC (CSIC-USAL), Salamanca, Spain;"}]},{"given":"Julia","family":"Almeida","sequence":"additional","affiliation":[{"name":"Servicio de Citometr\u00eda & Departamento de Medicina, Universidad de Salamanca (USAL), Spain;"},{"name":"Instituto de Biolog\u00eda Molecular y Celular del C\u00e1ncer, Centro de Investigaci\u00f3n del C\u00e1ncer\/IBMCC (CSIC-USAL), Salamanca, Spain;"}]},{"given":"Alberto","family":"Orfao","sequence":"additional","affiliation":[{"name":"Servicio de 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