{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T04:07:08Z","timestamp":1772165228830,"version":"3.50.1"},"reference-count":28,"publisher":"American Society of Hematology","issue":"7","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2007,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Understanding the distribution, function, and lineage relationship of CD8+ T-cell subpopulations is of fundamental value for the monitoring of the immune system in several experimental and clinical situations. However, the available data concerning the description of effector and memory CD8+ subsets in humans remain rather fragmentary because different studies favored the usage of distinct and restricted sets of cell surface markers and functional parameters. We associated multiple markers to subdivide CD8+ T cells into 14 different cell types, several of which were not described previously, and evaluated the coexpression of 18 genes simultaneously in individual cells from each subset. Our results show that each subset has a defined pattern of gene expression. Moreover, effector gene expression of CCR7\u2212 cells correlated only with CD27 expression levels and CD27\/CD28 coexpression but not with CD45RA\/R0 phenotypes. Our findings thus describe new CD8+ cell subsets, allow the identification of relatively homogeneous CD8+ subpopulations, provide a predictable and precise correlation between particular cell surface markers and CD8+ T-cell functional properties, and identify effector cells present in both CCR7\u2212CD45RA+ and CCR7\u2212CD45R0+ compartments. The results also indicate that activated cells might modulate the expression of CD45RA\/R0 asynchronously rather than CCR7\u2212CD45RA+ cells always issuing from CD45RA\u2212 precursors.<\/jats:p>","DOI":"10.1182\/blood-2006-06-027060","type":"journal-article","created":{"date-parts":[[2006,12,6]],"date-time":"2006-12-06T01:04:06Z","timestamp":1165367046000},"page":"2863-2870","update-policy":"https:\/\/doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":33,"title":["Cartography of gene expression in CD8 single cells: novel CCR7\u2212 subsets suggest differentiation independent of CD45RA expression"],"prefix":"10.1182","volume":"109","author":[{"given":"Marta","family":"Monteiro","sequence":"first","affiliation":[{"name":"Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale (INSERM) Unit 591, Necker Institute, Rene\u0301-Descartes Medical School, Paris, France;"}]},{"given":"Ce\u0301sar","family":"Evaristo","sequence":"additional","affiliation":[{"name":"Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale (INSERM) Unit 591, Necker Institute, Rene\u0301-Descartes Medical School, Paris, France;"}]},{"given":"Agne\u0300s","family":"Legrand","sequence":"additional","affiliation":[{"name":"Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale (INSERM) Unit 591, Necker Institute, Rene\u0301-Descartes Medical School, Paris, France;"}]},{"given":"Antonino","family":"Nicoletti","sequence":"additional","affiliation":[{"name":"INSERM Unit 681, Cordeliers Institute, Paris, France"}]},{"given":"Benedita","family":"Rocha","sequence":"additional","affiliation":[{"name":"Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale (INSERM) Unit 591, Necker Institute, Rene\u0301-Descartes Medical School, Paris, France;"}]}],"member":"234","published-online":{"date-parts":[[2006,12,5]]},"reference":[{"key":"2019111722493414800_B1","doi-asserted-by":"crossref","unstructured":"Hamann D, Baars PA, Rep MH, et al. 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