{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T06:22:35Z","timestamp":1777962155176,"version":"3.51.4"},"reference-count":53,"publisher":"American Society of Hematology","issue":"20","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2009,5,14]]},"abstract":"<jats:p>Endothelial cells contain cigar-shaped secretory organelles called Weibel-Palade bodies (WPBs) that play a crucial role in both hemostasis and the initiation of inflammation. The major cargo protein of WPBs is von Willebrand factor (VWF). In unstimulated cells, this protein is stored in a highly multimerized state coiled into protein tubules, but after secretagogue stimulation and exocytosis it unfurls, under shear force, as long platelet-binding strings. Small GTPases of the Rab family play a key role in organelle function. Using siRNA depletion in primary endothelial cells, we have identified a role for the WPB-associated Rab27a and its effector MyRIP. Both these proteins are present on only mature WPBs, and this rab\/effector complex appears to anchor these WPBs to peripheral actin. Depletion of either the Rab or its effector results in a loss of peripheral WPB localization, and this destabilization is coupled with an increase in both basal and stimulated secretion. The VWF released from Rab27a-depleted cells is less multimerized, and the VWF strings seen under flow are shorter. Our results indicate that this Rab\/effector complex controls peripheral distribution and prevents release of incompletely processed WPB content.<\/jats:p>","DOI":"10.1182\/blood-2008-09-181206","type":"journal-article","created":{"date-parts":[[2009,3,6]],"date-time":"2009-03-06T21:55:41Z","timestamp":1236376541000},"page":"5010-5018","update-policy":"https:\/\/doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":94,"title":["Rab27a and MyRIP regulate the amount and multimeric state of VWF released from endothelial cells"],"prefix":"10.1182","volume":"113","author":[{"given":"Thomas D.","family":"Nightingale","sequence":"first","affiliation":[{"name":"Medical Research Council Laboratory of Molecular Cell Biology, Cell Biology Unit and Department of Cell and Developmental Biology, University College London, London, United Kingdom"}]},{"given":"Krupa","family":"Pattni","sequence":"additional","affiliation":[{"name":"Medical Research Council Laboratory of 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