{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T08:12:01Z","timestamp":1768810321574,"version":"3.49.0"},"reference-count":48,"publisher":"American Society of Hematology","issue":"25","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2011,12,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Human mature dendritic cells (DCs) can efficiently stimulate natural killer (NK)\u2013cell responses without being targeted by their cytotoxicity. To understand this important regulatory crosstalk, we characterized the development of the immunologic synapse between mature DCs and resting NK cells. Conjugates between these 2 innate leukocyte populations formed rapidly, persisted for prolonged time periods and matured with DC-derived f-actin polymerization at the synapse. Polarization of IL-12 and IL-12R to the synapse coincided with f-actin polymerization, while other activating and inhibitory molecules were enriched at the interface between DCs and NK cells earlier. Functional assays revealed that inhibition of f-actin polymerization in mature synapses led to an increase of IFN-\u03b3 secretion and cytotoxicity by NK cells. This elevated NK-cell reactivity resulted from decreased inhibitory signaling in the absence of MHC class I polarization at the interface, which was observed on inhibition of f-actin polymerization in DCs. Thus, inhibitory signaling is stabilized by f-actin at the synapse between mature DCs and resting NK cells.<\/jats:p>","DOI":"10.1182\/blood-2011-07-366328","type":"journal-article","created":{"date-parts":[[2011,9,15]],"date-time":"2011-09-15T07:53:52Z","timestamp":1316073232000},"page":"6487-6498","update-policy":"https:\/\/doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":42,"title":["Cytoskeletal stabilization of inhibitory interactions in immunologic synapses of mature human dendritic cells with natural killer cells"],"prefix":"10.1182","volume":"118","author":[{"given":"Rosa","family":"Barreira da Silva","sequence":"first","affiliation":[{"name":"Viral Immunobiology, Institute of Experimental Immunology, University of Z\u00fcrich, Z\u00fcrich, Switzerland;"},{"name":"Instituto de Ci\u00eancias Biom\u00e9dicas Abel Salazar and Graduate Program in Areas of Basic and Applied Biology (GABBA), University of Porto, Porto, Portugal; and"}]},{"given":"Claudine","family":"Graf","sequence":"additional","affiliation":[{"name":"III Medizinische Klinik, University Medical Center of the Johannes Gutenberg University, Mainz, Germany"}]},{"given":"Christian","family":"M\u00fcnz","sequence":"additional","affiliation":[{"name":"Viral Immunobiology, Institute of Experimental Immunology, University of Z\u00fcrich, Z\u00fcrich, Switzerland;"}]}],"member":"234","reference":[{"issue":"6673","key":"2019111808154413100_B1","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1038\/32588","article-title":"Dendritic cells and the control of immunity.","volume":"392","author":"Banchereau","year":"1998","journal-title":"Nature"},{"issue":"3","key":"2019111808154413100_B2","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/j.immuni.2008.08.006","article-title":"Mechanisms and consequences of dendritic cell 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