{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T07:15:54Z","timestamp":1768288554319,"version":"3.49.0"},"reference-count":49,"publisher":"American Society of Hematology","issue":"25","content-domain":{"domain":["ashpublications.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2012,6,21]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Mouse CD8\u03b1+ dendritic cells (DCs) in lymphoid organs and CD103+ CD11b\u2212 DCs in nonlymphoid tissues share phenotypic and functional similarities, as well as a unique shared developmental dependence on the transcription factor Batf3. Human DCs resembling mouse CD8\u03b1+ DCs in phenotype and function have been identified in human blood, spleen, and tonsil. However, it is not clear whether such cells are also present in human nonlymphoid organs, and their equivalence to mouse CD8\u03b1+ DC has recently been questioned. Furthermore, the identification of \u201cCD8\u03b1+ DC-like\u201d cells across different tissues and species remains problematic because of the lack of a unique marker that can be used to unambiguously define lineage members. Here we show that mouse CD8\u03b1+ DCs and CD103+ CD11b\u2212 DCs can be defined by shared high expression of DNGR-1 (CLEC9A). We further show that DNGR-1 uniquely marks a CD11b\u2212 human DC population present in both lymphoid and nonlymphoid tissues of humans and humanized mice. Finally, we demonstrate that knockdown of Batf3 selectively prevents the development of DNGR-1+ human DCs in vitro. Thus, high expression of DNGR-1 specifically and universally identifies a unique DC subset in mouse and humans. Evolutionarily conserved Batf3 dependence justifies classification of DNGR-1hi DCs as a distinct DC lineage.<\/jats:p>","DOI":"10.1182\/blood-2012-01-406967","type":"journal-article","created":{"date-parts":[[2012,3,23]],"date-time":"2012-03-23T11:50:36Z","timestamp":1332503436000},"page":"6052-6062","update-policy":"https:\/\/doi.org\/10.1182\/blood.2019cm0000","source":"Crossref","is-referenced-by-count":211,"title":["DNGR-1 is a specific and universal marker of mouse and human Batf3-dependent dendritic cells in lymphoid and nonlymphoid tissues"],"prefix":"10.1182","volume":"119","author":[{"given":"Lionel F.","family":"Poulin","sequence":"first","affiliation":[{"name":"Immunobiology Laboratory and"}]},{"given":"Yasmin","family":"Reyal","sequence":"additional","affiliation":[{"name":"Haematopoietic Stem Cell Laboratory, Cancer Research UK, London Research Institute, London, United Kingdom;"}]},{"given":"Heli","family":"Uronen-Hansson","sequence":"additional","affiliation":[{"name":"Immunology Section, Lund University, Lund, Sweden;"}]},{"given":"Barbara U.","family":"Schraml","sequence":"additional","affiliation":[{"name":"Immunobiology Laboratory and"}]},{"given":"David","family":"Sancho","sequence":"additional","affiliation":[{"name":"Department of Vascular Biology and Inflammation, CNIC-Centro National de Investigaciones Cardiovasculares, Madrid, Spain;"}]},{"given":"Kenneth M.","family":"Murphy","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute and Department of Pathology and Immunology, Washington University School of Medicine, St Louis, MO;"}]},{"given":"Ulf K.","family":"H\u00e5kansson","sequence":"additional","affiliation":[{"name":"Department of Urology, Sk\u00e5ne University Hospital, Malm\u00f6, Sweden; and"}]},{"given":"Luis","family":"Ferreira Moita","sequence":"additional","affiliation":[{"name":"Cell Biology of the Immune System Unit, Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisbon, Portugal"}]},{"given":"William W.","family":"Agace","sequence":"additional","affiliation":[{"name":"Immunology Section, Lund University, Lund, Sweden;"}]},{"given":"Dominique","family":"Bonnet","sequence":"additional","affiliation":[{"name":"Haematopoietic Stem Cell Laboratory, Cancer Research UK, London Research Institute, London, United Kingdom;"}]},{"given":"Caetano","family":"Reis e Sousa","sequence":"additional","affiliation":[{"name":"Immunobiology Laboratory and"}]}],"member":"234","reference":[{"issue":"1","key":"2019111808270537500_B1","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.coi.2010.01.022","article-title":"Intracellular mechanisms of antigen cross presentation in dendritic cells.","volume":"22","author":"Amigorena","year":"2010","journal-title":"Curr Opin 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