{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T23:47:40Z","timestamp":1782776860441,"version":"3.54.5"},"reference-count":31,"publisher":"European Respiratory Society (ERS)","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur Respir J"],"accepted":{"date-parts":[[2009,3,18]]},"published-print":{"date-parts":[[2009,11]]},"abstract":"<jats:p>The research question addressed in the current study was: does the pneumococcal pore-forming toxin, pneumolysin, mobilise matrix metalloproteinase (MMP) -8 and -9 from isolated human blood neutrophils at sublytic concentrations of 5, 10 and 20\u2005ng\u00b7mL<jats:sup>\u22121<\/jats:sup>?<\/jats:p><jats:p>MMPs were measured in the supernatants of unstimulated neutrophils and of cells exposed to pneumolysin and the chemoattractant<jats:italic>N<\/jats:italic>-formyl-<jats:sc>l<\/jats:sc>-methionyl-<jats:sc>l<\/jats:sc>-leucyl-<jats:sc>l<\/jats:sc>-phenylalanine (f-MLP; 0.1\u2005\u03bcM), individually and in combination, using ELISA procedures, and alterations in cytosolic Ca<jats:sup>2+<\/jats:sup>concentrations were monitored using a fura-2 acetoxymethyl ester (fura-2\/AM)-based spectrofluorimetric method.<\/jats:p><jats:p>Treatment of neutrophils with pneumolysin alone caused dose-related release of both MMPs, whereas f-MLP caused modest increases; the combination of both activators was, however, most effective. Pneumolysin\/f-MLP-activated release of the MMPs from the cells was paralleled by increases in cytosolic Ca<jats:sup>2+<\/jats:sup>.<\/jats:p><jats:p>Exposure of human neutrophils to pneumolysin is accompanied by mobilisation of MMPs, which is potentiated by f-MLP. If operative<jats:italic>in vivo<\/jats:italic>, pneumolysin-mediated release of MMPs from neutrophils and other cell types may contribute to the pathogenesis of severe pneumococcal disease.<\/jats:p>","DOI":"10.1183\/09031936.00007109","type":"journal-article","created":{"date-parts":[[2009,10,30]],"date-time":"2009-10-30T16:14:27Z","timestamp":1256919267000},"page":"1167-1170","source":"Crossref","is-referenced-by-count":14,"title":["Pneumolysin induces release of matrix metalloproteinase-8 and -9 from human neutrophils"],"prefix":"10.1183","volume":"34","author":[{"given":"R.","family":"Cockeran","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. 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