{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T08:15:39Z","timestamp":1791188139660,"version":"4.1.0"},"reference-count":24,"publisher":"European Respiratory Society (ERS)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur Respir J"],"accepted":{"date-parts":[[2010,5,30]]},"published-print":{"date-parts":[[2011,3]]},"abstract":"<jats:p>Early detection of the cyanobacterium<jats:italic>Pseudomonas aeruginosa<\/jats:italic>in the lungs of young children with cystic fibrosis (CF) is considered the key to delaying chronic pulmonary disease. We investigated whether cyanide in bronchoalveolar lavage (BAL) fluid could be used as an early diagnostic biomarker of infection.<\/jats:p><jats:p>Cyanide was measured in 226 BAL samples (36<jats:italic>P. aeruginosa<\/jats:italic>infected) obtained from 96 infants and young children with CF participating in an early surveillance programme involving annual BAL.<\/jats:p><jats:p>Cyanide was detected in 97.2% of<jats:italic>P. aeruginosa<\/jats:italic>infected and 60.5% of uninfected samples. Cyanide concentrations were significantly higher in BALs infected with<jats:italic>P. aeruginosa<\/jats:italic>(median (25th\u201375th percentile) 27.3 (22.1\u201333.3) \u03bcM) than those which were not (17.2 (7.85\u201323.0) \u03bcM, p&lt;0.001). The best sensitivity, specificity, positive and negative predictive values were obtained with a cut-off concentration of 20.6 \u03bcM, and were 83%, 66%, 32% and 96%, respectively. Neutrophil number in BAL was a significant predictor of cyanide concentration (p&lt;0.001).<\/jats:p><jats:p>Cyanide concentration can distinguish between<jats:italic>P. aeruginosa<\/jats:italic>infected and uninfected BALs as a group, but not individually; therefore, cyanide is a poor diagnostic biomarker of<jats:italic>P. aeruginosa<\/jats:italic>infection. Cyanide levels in BAL are related to the level of neutrophilic inflammation.<\/jats:p>","DOI":"10.1183\/09031936.00024210","type":"journal-article","created":{"date-parts":[[2010,6,21]],"date-time":"2010-06-21T00:20:46Z","timestamp":1277079646000},"page":"553-558","source":"Crossref","is-referenced-by-count":30,"title":["Cyanide in bronchoalveolar lavage is not diagnostic for<i>Pseudomonas aeruginosa<\/i>in children with cystic fibrosis"],"prefix":"10.1183","volume":"37","author":[{"given":"M.D.","family":"Stutz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.L.","family":"Gangell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.J.","family":"Berry","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.W.","family":"Garratt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Sheil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.D.","family":"Sly","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"81","published-online":{"date-parts":[[2010,6,18]]},"reference":[{"key":"2024102020210763000_37.3.553.1","doi-asserted-by":"publisher","DOI":"10.1002\/ppul.2009.abs"},{"key":"2024102020210763000_37.3.553.2","doi-asserted-by":"publisher","DOI":"10.1002\/ppul.10127"},{"key":"2024102020210763000_37.3.553.3","doi-asserted-by":"publisher","DOI":"10.1067\/mpd.2001.112897"},{"key":"2024102020210763000_37.3.553.4","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00043108"},{"key":"2024102020210763000_37.3.553.5","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcf.2008.06.009"},{"key":"2024102020210763000_37.3.553.6","doi-asserted-by":"publisher","DOI":"10.1016\/j.prrv.2008.01.002"},{"key":"2024102020210763000_37.3.553.7","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(63)90019-0"},{"key":"2024102020210763000_37.3.553.8","doi-asserted-by":"publisher","DOI":"10.1007\/s002039900127"},{"key":"2024102020210763000_37.3.553.9","doi-asserted-by":"crossref","first-page":"1344","DOI":"10.1139\/m83-209","article-title":"Hydrogen cyanide production by Pseudomonas aeruginosa at reduced oxygen levels","volume":"29","author":"Castric","year":"1983","journal-title":"Can J Microbiol"},{"key":"2024102020210763000_37.3.553.10","doi-asserted-by":"publisher","DOI":"10.1172\/JCI200213870"},{"key":"2024102020210763000_37.3.553.11","doi-asserted-by":"publisher","DOI":"10.1002\/ppul.20963"},{"key":"2024102020210763000_37.3.553.12","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00159607"},{"key":"2024102020210763000_37.3.553.13","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00152407"},{"key":"2024102020210763000_37.3.553.14","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200901-0069OC"},{"key":"2024102020210763000_37.3.553.15","unstructured":"Australian Respiratory Early Surveillance Team for Cystic Fibrosis. 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