{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T06:21:12Z","timestamp":1792822872096,"version":"4.5.0"},"reference-count":32,"publisher":"European Respiratory Society (ERS)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur Respir J"],"accepted":{"date-parts":[[2009,7,24]]},"published-print":{"date-parts":[[2010,2]]},"abstract":"<jats:p>The presence of somatic mutations of the tyrosine kinase domain of the epidermal growth factor receptor (EGFR) gene in patients with advanced nonsmall cell lung cancer (NSCLC) correlates with a good response to tyrosine kinase inhibitors. The usefulness of endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) for the detection of EGFR mutations in cells recovered from malignant mediastinal nodes in patients with NSCLC was assessed.<\/jats:p><jats:p>All patients with lung adenocarcinoma or unspecified NSCLC referred for staging with EBUS-TBNA were included. Nodes with a short-axis diameter of &gt;5\u2005mm were sampled, and genomic DNA from metastatic tumour cells was obtained for analysis of exons 19 and 21. The impact of sampling on management was assessed.<\/jats:p><jats:p>EGFR gene analysis of the EBUS-TBNA sample was feasible in 26 (72.2%) out of the 36 patients with lymph node metastasis. Somatic mutations of the EGFR gene were detected in tissue obtained through EBUS-TBNA in two (10%) out of 20 patients with metastasic lung adenocarcinoma.<\/jats:p><jats:p>Malignant tissue samples obtained by EBUS-TBNA from patients with nodal metastasis of NSCLC are suitable for the detection of EGFR mutations in most cases, and this technique demonstrates mutated neoplastic cells in a tenth of patients with adenocarcinoma.<\/jats:p>","DOI":"10.1183\/09031936.00028109","type":"journal-article","created":{"date-parts":[[2009,7,31]],"date-time":"2009-07-31T01:38:33Z","timestamp":1249004313000},"page":"391-395","source":"Crossref","is-referenced-by-count":100,"title":["Endobronchial ultrasound-guided transbronchial needle aspiration for identifying EGFR mutations"],"prefix":"10.1183","volume":"35","author":[{"given":"I.","family":"Garcia-Oliv\u00e9","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Mons\u00f3","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Andreo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Sanz-Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Taron","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. A.","family":"Molina-Vila","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Llatj\u00f3s","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Castell\u00e0","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Moran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Bertran-Alamillo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Mayo-de-las-Casas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Queralt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Rosell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"81","published-online":{"date-parts":[[2009,7,30]]},"reference":[{"key":"2024102020000883000_35.2.391.1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-04-0189"},{"key":"2024102020000883000_35.2.391.2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa040938"},{"key":"2024102020000883000_35.2.391.3","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdi221"},{"key":"2024102020000883000_35.2.391.4","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-04-2618"},{"key":"2024102020000883000_35.2.391.5","doi-asserted-by":"publisher","DOI":"10.1016\/j.lungcan.2005.05.017"},{"key":"2024102020000883000_35.2.391.6","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2005.01.388"},{"key":"2024102020000883000_35.2.391.7","doi-asserted-by":"crossref","unstructured":"Mitsudomi T, Kosaka T, Endoh H, et al. Mutations of the epidermal growth factor receptor gene predict prolonged survival after gefitinib treatment in patients with non-small-cell lung cancer. J Clin Oncol 2005:2513\u20132520.","DOI":"10.1200\/JCO.2005.00.992"},{"key":"2024102020000883000_35.2.391.8","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.21458"},{"key":"2024102020000883000_35.2.391.9","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0005133"},{"key":"2024102020000883000_35.2.391.10","doi-asserted-by":"crossref","first-page":"936","DOI":"10.1097\/JTO.0b013e3181a9a0a2","volume":"4","year":"2009","journal-title":"J Thorac Oncol"},{"key":"2024102020000883000_35.2.391.11","doi-asserted-by":"crossref","unstructured":"Kaira K, Naito T, Takahashi T, et al. Pooled analysis of the reports of erlotinib after failure of gefitinib for non-small cell lung cancer. Lung Cancer 2009; [Epub ahead of print DOI: 10.1016\/j.lungcan.2009.05.006]","DOI":"10.1016\/j.lungcan.2009.05.006"},{"key":"2024102020000883000_35.2.391.12","doi-asserted-by":"publisher","DOI":"10.1007\/s00280-009-0973-1"},{"key":"2024102020000883000_35.2.391.13","doi-asserted-by":"publisher","DOI":"10.1378\/chest.06-1673"},{"key":"2024102020000883000_35.2.391.14","doi-asserted-by":"publisher","DOI":"10.1378\/chest.07-0095"},{"key":"2024102020000883000_35.2.391.15","doi-asserted-by":"publisher","DOI":"10.1378\/chest.126.1.122"},{"key":"2024102020000883000_35.2.391.16","doi-asserted-by":"publisher","DOI":"10.1016\/j.lungcan.2005.07.013"},{"key":"2024102020000883000_35.2.391.17","doi-asserted-by":"publisher","DOI":"10.1378\/chest.106.5.1538"},{"key":"2024102020000883000_35.2.391.18","doi-asserted-by":"crossref","first-page":"1349","DOI":"10.2214\/ajr.164.6.7754872","volume":"164","year":"1995","journal-title":"AJR Am J Roentgenol"},{"key":"2024102020000883000_35.2.391.19","doi-asserted-by":"publisher","DOI":"10.1378\/chest.101.1.244"},{"key":"2024102020000883000_35.2.391.20","doi-asserted-by":"publisher","DOI":"10.1136\/thorax.56.1.1"},{"key":"2024102020000883000_35.2.391.21","doi-asserted-by":"publisher","DOI":"10.1016\/j.ultrasmedbio.2009.03.007"},{"key":"2024102020000883000_35.2.391.22","first-page":"517","volume":"34","year":"1990","journal-title":"Acta Cytol"},{"key":"2024102020000883000_35.2.391.23","doi-asserted-by":"publisher","DOI":"10.1097\/JTO.0b013e318189f579"},{"key":"2024102020000883000_35.2.391.24","doi-asserted-by":"publisher","DOI":"10.1016\/S0163-7258(98)00045-X"},{"key":"2024102020000883000_35.2.391.25","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.22012"},{"key":"2024102020000883000_35.2.391.26","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6603396"},{"key":"2024102020000883000_35.2.391.27","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2006.00216.x"},{"key":"2024102020000883000_35.2.391.28","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-05-2324"},{"key":"2024102020000883000_35.2.391.29","doi-asserted-by":"publisher","DOI":"10.1126\/science.1099314"},{"key":"2024102020000883000_35.2.391.30","doi-asserted-by":"crossref","first-page":"1167","DOI":"10.1158\/1078-0432.1167.11.3","volume":"11","year":"2005","journal-title":"Clin Cancer Res"},{"key":"2024102020000883000_35.2.391.31","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2008.17.3930"},{"key":"2024102020000883000_35.2.391.32","doi-asserted-by":"publisher","DOI":"10.1016\/j.lungcan.2008.10.005"}],"container-title":["European Respiratory Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1183\/09031936.00028109","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,24]],"date-time":"2025-02-24T06:27:49Z","timestamp":1740378469000},"score":1,"resource":{"primary":{"URL":"https:\/\/publications.ersnet.org\/lookup\/doi\/10.1183\/09031936.00028109"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,7,30]]},"references-count":32,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2010,2,1]]},"published-print":{"date-parts":[[2010,2]]}},"alternative-id":["10.1183\/09031936.00028109"],"URL":"https:\/\/doi.org\/10.1183\/09031936.00028109","relation":{},"ISSN":["0903-1936","1399-3003"],"issn-type":[{"value":"0903-1936","type":"print"},{"value":"1399-3003","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,7,30]]}}}