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DDR1 is responsible for maintaining the normal structure of skin and kidney epithelia and we hypothesised that DDR1 plays a regulatory role in bronchial epithelial integrity by transducing signals from the airway ECM.<\/jats:p><jats:p>Effects of DDR1 depletion were studied using RNA interference in primary human bronchial epithelial cells (HBECs) and BEAS-2B cells. The effects of overexpression of DDR1a and DDR1b in BEAS-2B cells were studied using a plasmid vector. We measured the effects on epithelial repair using a scratch wounding model, and levels of matrix metalloproteinases (MMPs) by gelatin zymography (MMP-2 and -9) and ELISA (MMP-7).<\/jats:p><jats:p>We showed that knockdown of DDR1 slowed epithelial repair by 50%, which was associated with a reduction in levels of MMP-7, whilst DDR1 overexpression enhanced epithelial repair. DDR1 knockdown reduced proliferation of HBECs, but had no significant effect on adhesion to collagen I or other matrix substrates.<\/jats:p><jats:p>These data suggest that ECM signalling<jats:italic>via<\/jats:italic>DDR1 regulates aspects of bronchial epithelial repair, integrity and MMP expression in the airways.<\/jats:p>","DOI":"10.1183\/09031936.00039710","type":"journal-article","created":{"date-parts":[[2010,10,1]],"date-time":"2010-10-01T01:11:38Z","timestamp":1285895498000},"page":"1482-1493","source":"Crossref","is-referenced-by-count":39,"title":["Discoidin domain receptor 1 regulates bronchial epithelial repair and matrix metalloproteinase 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