{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T02:49:56Z","timestamp":1793674196012,"version":"4.5.1"},"reference-count":30,"publisher":"European Respiratory Society (ERS)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur Respir J"],"accepted":{"date-parts":[[2010,10,11]]},"published-print":{"date-parts":[[2011,3]]},"abstract":"<jats:p>No study has evaluated the correlation between different expression of nitric oxide synthase (NOS) isoforms in nasal epithelial cells and nasal NO (nNO) level in primary ciliary dyskinesia (PCD).<\/jats:p>\n                  <jats:p>Gene expression of endothelial (NOS3) and inducible NOS (NOS2) and their correlation with nNO level, ciliary function and morphology were studied in patients with PCD or secondary ciliary dyskinesia (SCD). NOS3 gene polymorphisms were studied in blood leukocytes.<\/jats:p>\n                  <jats:p>A total of 212 subjects were studied (48 with PCD, 161 with SCD and three normal subjects). nNO level correlated with mean ciliary beat frequency (p = 0.044; r = 0.174). The lower the nNO level the higher was the percentage of immotile cilia (p&lt;0.001; r = -0.375). A significant positive correlation between NOS2 gene expression and nNO levels was demonstrated in all children (p = 0.001; r = 0.428), and this correlation was confirmed in patients with PCD (p = 0.019; r = 0.484). NOS2 gene expression was lower in PCD than in SCD (p = 0.04). The NOS3 isoform correlated with missing central microtubules (p = 0.048; r = 0.447). nNO levels were higher in PCD subjects with the NOS3 thymidine 894 mutation, and this was associated with a higher ciliary beat frequency (p = 0.045).<\/jats:p>\n                  <jats:p>These results demonstrate a relationship between nNO level, NOS mRNA expression and ciliary beat frequency.<\/jats:p>","DOI":"10.1183\/09031936.00044310","type":"journal-article","created":{"date-parts":[[2011,2,22]],"date-time":"2011-02-22T14:20:53Z","timestamp":1298384453000},"page":"572-577","source":"Crossref","is-referenced-by-count":45,"title":["Nasal nitric oxide and nitric oxide synthase expression in primary ciliary dyskinesia"],"prefix":"10.1183","volume":"37","author":[{"given":"M.","family":"Pifferi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Bush","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Maggi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Michelucci","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Ricci","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.E.","family":"Conidi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.M.","family":"Cangiotti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Bodini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Simi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Macchia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.L.","family":"Boner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"81","published-online":{"date-parts":[[2011,1,27]]},"reference":[{"key":"2024102020325488000_37.3.572.1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00176608"},{"key":"2024102020325488000_37.3.572.2","doi-asserted-by":"publisher","DOI":"10.1136\/thorax.57.7.586"},{"key":"2024102020325488000_37.3.572.3","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00001010"},{"key":"2024102020325488000_37.3.572.4","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00068810"},{"key":"2024102020325488000_37.3.572.5","first-page":"18","article-title":"Nitric oxide production in the sphenoidal sinus by the inducible and constitutive isozymes of nitric oxide synthase","volume":"43","author":"Petruson","year":"2005","journal-title":"Rhinology"},{"key":"2024102020325488000_37.3.572.6","doi-asserted-by":"publisher","DOI":"10.1002\/ppul.20670"},{"key":"2024102020325488000_37.3.572.7","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1096\/fasebj.12.10.773","article-title":"Expressional control of the \u201cconstitutive\u201d isoforms of nitric oxide synthase (NOS I and NOS III)","volume":"12","author":"Forstermann","year":"1998","journal-title":"FASEB J"},{"key":"2024102020325488000_37.3.572.8","doi-asserted-by":"crossref","first-page":"17478","DOI":"10.1016\/S0021-9258(19)85359-0","article-title":"Structure and chromosomal localization of the human constitutive endothelial nitric oxide synthase gene","volume":"268","author":"Marsden","year":"1993","journal-title":"J Biol Chem"},{"key":"2024102020325488000_37.3.572.9","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.6.2832"},{"key":"2024102020325488000_37.3.572.10","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200212-1491BC"},{"key":"2024102020325488000_37.3.572.11","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200202-155OC"},{"key":"2024102020325488000_37.3.572.12","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.02.00293102"},{"key":"2024102020325488000_37.3.572.13","doi-asserted-by":"publisher","DOI":"10.1016\/j.rmed.2007.11.013"},{"key":"2024102020325488000_37.3.572.14","doi-asserted-by":"publisher","DOI":"10.1046\/j.0905-6157.2001.00000.x"},{"key":"2024102020325488000_37.3.572.15","doi-asserted-by":"crossref","first-page":"653","DOI":"10.3109\/01913128709048451","article-title":"Ultrastructural diagnosis in the immotile cilia syndrome","volume":"11","author":"Carl\u00e9n","year":"1987","journal-title":"Ultrastruct Pathol"},{"key":"2024102020325488000_37.3.572.16","doi-asserted-by":"publisher","DOI":"10.1136\/thx.2008.110940"},{"key":"2024102020325488000_37.3.572.17","unstructured":"RANDOM.ORG. www.random.org Date last updated: April 2007. 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