{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T16:58:16Z","timestamp":1790528296261,"version":"4.1.0"},"reference-count":31,"publisher":"European Respiratory Society (ERS)","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur Respir J"],"accepted":{"date-parts":[[2008,6,26]]},"published-print":{"date-parts":[[2008,12]]},"abstract":"<jats:p>Passive depletion of internal Ca<jats:sup>2+<\/jats:sup>stores in airway smooth muscle (ASM) activates nonselective cation channels (NSCCs) that mediate capacitative Ca<jats:sup>2+<\/jats:sup>entry. However, the single channel properties of these cation channels have yet to be resolved and their regulation by cytosolic Ca<jats:sup>2+<\/jats:sup>levels ([Ca<jats:sup>2+<\/jats:sup>]<jats:italic><jats:sub>i<\/jats:sub><\/jats:italic>) still remains unclear.<\/jats:p><jats:p>NSCC currents and changes in [Ca<jats:sup>2+<\/jats:sup>]<jats:italic><jats:sub>i<\/jats:sub><\/jats:italic>during passive depletion of internal Ca<jats:sup>2+<\/jats:sup>stores were monitored in isolated bovine tracheal myocytes.<\/jats:p><jats:p>Loading cells with 1,2-bis(<jats:italic>o<\/jats:italic>-aminophenoxy)ethane-<jats:italic>N,N,N\u2032,N\u2032<\/jats:italic>-tetracetic acid acetyl methyl ester (BAPTA-AM) to reduce [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>and thereby deplete the store augmented a basal Gd<jats:sup>3+<\/jats:sup>- and La<jats:sup>3+<\/jats:sup>-sensitive, Ca<jats:sup>2+<\/jats:sup>-permeable NSCC current. This current mimics that which is evoked by store depletion using the sarcoplasmic reticulum Ca<jats:sup>2+<\/jats:sup>pump inhibitor cyclopiazonic acid (which concurrently and transiently elevates [Ca<jats:sup>2+<\/jats:sup>]<jats:italic><jats:sub>i<\/jats:sub><\/jats:italic>). Both interventions activated an \u223c25-pS NSCC with properties identical to both spontaneous (basal) and BAPTA-AM-evoked single channel currents.<\/jats:p><jats:p>In summary, the present study provides novel evidence that a lanthanide-sensitive, 25-pS nonselective cation channel underlies both basal and store depletion-evoked membrane currents in airway smooth muscle and that this conductance likely contributes to the regulation of resting [Ca<jats:sup>2+<\/jats:sup>]<jats:italic><jats:sub>i<\/jats:sub><\/jats:italic>and capacitative Ca<jats:sup>2+<\/jats:sup>entry.<\/jats:p>","DOI":"10.1183\/09031936.00054608","type":"journal-article","created":{"date-parts":[[2008,7,10]],"date-time":"2008-07-10T00:24:54Z","timestamp":1215649494000},"page":"1529-1538","source":"Crossref","is-referenced-by-count":23,"title":["Properties of a store-operated nonselective cation channel in airway smooth muscle"],"prefix":"10.1183","volume":"32","author":[{"given":"P. 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