{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T19:41:55Z","timestamp":1790797315192,"version":"4.1.0"},"reference-count":32,"publisher":"European Respiratory Society (ERS)","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur Respir J"],"accepted":{"date-parts":[[2012,7,22]]},"published-print":{"date-parts":[[2013,5]]},"abstract":"<jats:p>\n                    We hypothesised that primary bronchial epithelial cells (PBECs) from subjects with chronic obstructive pulmonary disease (COPD) respond differently to\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    lipopolysaccharide (LPS) after cigarette smoke extract (CSE) exposure than PBECs obtained from smokers without airflow obstruction and nonsmokers.\n                  <\/jats:p>\n                  <jats:p>\n                    PBECs from 16 COPD subjects, 10 smokers without airflow obstruction and nine nonsmokers were cultured at air\u2013liquid interface. Cultures were incubated with CSE prior to stimulation with\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    LPS. Interleukin (IL)-6 and IL-8 were measured by ELISA and Toll-like receptor (TLR)-4 expression by fluorescence-activated cell sorter. Activation of nuclear factor (NF)-\u03baB was determined by Western blotting and ELISA, and MAPK and caspase-3 activity by Western blotting. Apoptosis was evaluated using Annexin-V staining and the terminal transferase-mediated dUTP nick end-labelling methods.\n                  <\/jats:p>\n                  <jats:p>\n                    Constitutive release of IL-8 and IL-6 was greatest from the COPD cultures. However, CSE pretreatment followed by\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    LPS stimulation reduced IL-8 release from COPD PBECs, but increased it from cells of smokers without airflow obstruction and nonsmokers. TLR-4 expression, MAPK and NF-\u03baB activation in COPD cultures were reduced after CSE treatment, but not in the smokers without airflow obstruction or nonsmoker groups, which was associated with increased apoptosis.\n                  <\/jats:p>\n                  <jats:p>CSE attenuates inflammatory responses to LPS in cells from people with COPD but not those from nonsmoking individuals and those who smoke without airflow obstruction.<\/jats:p>","DOI":"10.1183\/09031936.00063112","type":"journal-article","created":{"date-parts":[[2012,8,11]],"date-time":"2012-08-11T20:25:52Z","timestamp":1344716752000},"page":"1058-1067","source":"Crossref","is-referenced-by-count":92,"title":["Airway epithelial cell apoptosis and inflammation in COPD, smokers and nonsmokers"],"prefix":"10.1183","volume":"41","author":[{"given":"David M.","family":"Comer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph C.","family":"Kidney","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madeleine","family":"Ennis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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