{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,25]],"date-time":"2025-02-25T05:15:28Z","timestamp":1740460528274,"version":"3.37.3"},"reference-count":37,"publisher":"European Respiratory Society (ERS)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur Respir J"],"accepted":{"date-parts":[[2009,11,22]]},"published-print":{"date-parts":[[2010,8]]},"abstract":"<jats:p>The tuberculin skin test (TST) using purified protein derivative (PPD) of<jats:italic>Mycobacterium tuberculosis<\/jats:italic>is traditionally used to diagnose latent tuberculosis (TB) infection (LTBI). However, LTBI diagnosis by peripheral blood mononuclear cell (PBMC) interferon (IFN)-\u03b3 responses to<jats:italic>M. tuberculosis-<\/jats:italic>specific antigens, early secreted antigenic target 6 kDa (ESAT-6) and culture filtrate protein (CFP)-10 has greater specificity. We investigated the difference in antimycobacterium cellular immunity in TB contacts who were strong TST reactors but nonresponsive to the ESAT-6\/CFP-10 assay compared with those with concordant results.<\/jats:p><jats:p>Healthy TB contacts were tested using the above two assays and mycobacterium survival was measured after co-culture of infected macrophages with their PBMCs.<\/jats:p><jats:p>Whether PPD reactivity was tested by TST or by PBMC-specific IFN-\u03b3 responses, strongly PPD-reactive TB contacts without ESAT-6\/CFP-10 responsiveness showed significantly better mycobacterium inhibition activity than ESAT-6\/CFP-10-responsive TB contacts with the same PPD reactivity. In the former group, stronger PPD reactivity was associated with improved mycobacterium killing, whereas ESAT-6\/CFP-10 responders showed the opposite result.<\/jats:p><jats:p>PPD-reactive ESAT-6\/CFP-10-nonresponsive TB contacts in our population may have had protective immunity related to prior mycobacterium exposure. ESAT-6\/CFP10-responsive TB contacts are more likely to have LTBI and, in this group, strong PPD reactivity may paradoxically be associated with poor mycobactericidal activity.<\/jats:p>","DOI":"10.1183\/09031936.00063209","type":"journal-article","created":{"date-parts":[[2009,12,9]],"date-time":"2009-12-09T03:58:39Z","timestamp":1260331119000},"page":"348-354","source":"Crossref","is-referenced-by-count":1,"title":["Strong purified protein derivative responses are associated with poor mycobacterium inhibition in latent 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