{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T03:03:53Z","timestamp":1789959833289,"version":"4.0.1"},"reference-count":33,"publisher":"European Respiratory Society (ERS)","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur Respir J"],"accepted":{"date-parts":[[2009,9,1]]},"published-print":{"date-parts":[[2010,4]]},"abstract":"<jats:p>Basophils are the accessory cell type for T-helper (Th)2 induction and initiators in immunoglobulin E-mediated chronic allergic inflammation. Basophils and Th17 cells accumulate at the inflammatory sites, such as the airways of allergic asthmatic patients.<\/jats:p><jats:p>We investigated the activation of interleukin (IL)-17A on the primary human basophils\/KU812 basophilic cells and primary human bronchial epithelial cells\/BEAS-2B bronchial epithelial cells. Cytokines, chemokines, adhesion molecules and intracellular signalling molecules were assayed by ELISA or flow cytometry.<\/jats:p><jats:p>Co-culture of bronchial epithelial cells and basophils could significantly induce the release of IL-6, an epithelial inflammatory cytokine, and CCL2, a chemokine for basophils, esosinophils and monocytes. Such induction was synergistically enhanced by IL-17A, and direct interaction between these two cells was necessary for IL-17A-induced IL-6 and CCL2 release. Surface expression of intercellular adhesion molecule-1 on bronchial epithelial cells was also upregulated upon their interaction. The interaction of basophils and bronchial epithelial cells under IL-17A stimulation was differentially regulated by extracellular signal-regulated kinase, c-Jun N-terminal protein kinase, p38 mitogen-activated protein kinase and nuclear factor-\u03baB pathways.<\/jats:p><jats:p>These findings suggest a novel immunopathological role of Th17 cells and basophils in allergic asthma through the activation of granulocyte-mediated inflammation initiated by the direct interaction between basophils and bronchial epithelial cells.<\/jats:p>","DOI":"10.1183\/09031936.00088309","type":"journal-article","created":{"date-parts":[[2009,9,10]],"date-time":"2009-09-10T00:56:09Z","timestamp":1252544169000},"page":"883-893","source":"Crossref","is-referenced-by-count":40,"title":["Interleukin-17A activation on bronchial epithelium and basophils: a novel inflammatory mechanism"],"prefix":"10.1183","volume":"35","author":[{"given":"C. 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